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Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis

  • Authors:
    • Zhizhou Yang
    • Zhaorui Sun
    • Hongmei Liu
    • Yi Ren
    • Danbing Shao
    • Wei Zhang
    • Jinfeng Lin
    • Joy Wolfram
    • Feng Wang
    • Shinan Nie
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China, CAS Key Laboratory for Biomedial Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, University of Chinese Academy of Sciences, Beijing 100190, P.R. China, Department of Gastroenterology, The Tenth People's Hospital of Shanghai, Tongji University, Shanghai 200072, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1091-1097
    |
    Published online on: March 24, 2015
       https://doi.org/10.3892/mmr.2015.3537
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Abstract

It is well established that paraquat (PQ) poisoning can cause severe lung injury during the early stages of exposure, finally leading to irreversible pulmonary fibrosis. Connective tissue growth factor (CTGF) is an essential growth factor that is involved in tissue repair and pulmonary fibrogenesis. In the present study, the role of CTGF was examined in a rat model of pulmonary fibrosis induced by PQ poisoning. Histological examination revealed interstitial edema and extensive cellular thickening of interalveolar septa at the early stages of poisoning. At 2 weeks after PQ administration, lung tissue sections exhibited a marked thickening of the alveolar walls with an accumulation of interstitial cells with a fibroblastic appearance. Masson's trichrome staining revealed a patchy distribution of collagen deposition, indicating pulmonary fibrogenesis. Western blot analysis and immunohistochemical staining of tissue samples demonstrated that CTGF expression was significantly upregulated in the PQ‑treated group. Similarly, PQ treatment of MRC‑5 human lung fibroblast cells caused an increase in CTGF in a dose‑dependent manner. Furthermore, the addition of CTGF to MRC‑5 cells triggered cellular proliferation and migration. In addition, CTGF induced the differentiation of fibroblasts to myofibroblasts, as was evident from increased expression of α‑smooth muscle actin (α‑SMA) and collagen. These findings demonstrate that PQ causes increased CTGF expression, which triggers proliferation, migration and differentiation of lung fibroblasts. Therefore, CTGF may be important in PQ‑induced pulmonary fibrogenesis, rendering this growth factor a potential pharmacological target for reducing lung injury.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Z, Sun Z, Liu H, Ren Y, Shao D, Zhang W, Lin J, Wolfram J, Wang F, Nie S, Nie S, et al: Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis. Mol Med Rep 12: 1091-1097, 2015.
APA
Yang, Z., Sun, Z., Liu, H., Ren, Y., Shao, D., Zhang, W. ... Nie, S. (2015). Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis. Molecular Medicine Reports, 12, 1091-1097. https://doi.org/10.3892/mmr.2015.3537
MLA
Yang, Z., Sun, Z., Liu, H., Ren, Y., Shao, D., Zhang, W., Lin, J., Wolfram, J., Wang, F., Nie, S."Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis". Molecular Medicine Reports 12.1 (2015): 1091-1097.
Chicago
Yang, Z., Sun, Z., Liu, H., Ren, Y., Shao, D., Zhang, W., Lin, J., Wolfram, J., Wang, F., Nie, S."Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis". Molecular Medicine Reports 12, no. 1 (2015): 1091-1097. https://doi.org/10.3892/mmr.2015.3537
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Z, Sun Z, Liu H, Ren Y, Shao D, Zhang W, Lin J, Wolfram J, Wang F, Nie S, Nie S, et al: Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis. Mol Med Rep 12: 1091-1097, 2015.
APA
Yang, Z., Sun, Z., Liu, H., Ren, Y., Shao, D., Zhang, W. ... Nie, S. (2015). Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis. Molecular Medicine Reports, 12, 1091-1097. https://doi.org/10.3892/mmr.2015.3537
MLA
Yang, Z., Sun, Z., Liu, H., Ren, Y., Shao, D., Zhang, W., Lin, J., Wolfram, J., Wang, F., Nie, S."Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis". Molecular Medicine Reports 12.1 (2015): 1091-1097.
Chicago
Yang, Z., Sun, Z., Liu, H., Ren, Y., Shao, D., Zhang, W., Lin, J., Wolfram, J., Wang, F., Nie, S."Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat‑induced pulmonary fibrosis". Molecular Medicine Reports 12, no. 1 (2015): 1091-1097. https://doi.org/10.3892/mmr.2015.3537
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