Attenuation of Porphyromonas gingivalis oral infection by α‑amylase and pentamidine

Li,Ying; Miao,Yu‑Song; Fu,Yun; Li,Xi‑Ting; Yu,Shao‑Jie

doi:10.3892/mmr.2015.3584

August-2015 Volume 12 Issue 2

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August-2015 Volume 12 Issue 2

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Article

Attenuation of Porphyromonas gingivalis oral infection by α‑amylase and pentamidine

Authors:
- Ying Li
- Yu‑Song Miao
- Yun Fu
- Xi‑Ting Li
- Shao‑Jie Yu
View Affiliations / Copyright

Affiliations: Department of Periodontology, Guanghua School of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Dental Science, Guangzhou Chest Hospital, Guangzhou, Guangdong 510055, P.R. China
Pages: 2155-2160
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Published online on: April 1, 2015

https://doi.org/10.3892/mmr.2015.3584
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Abstract

The Porphyromonas gingivalis bacterium is one of the most influential pathogens in oral infections. In the current study, the antimicrobial activity of α‑amylase and pentamidine against Porphyromonas gingivalis was evaluated. Their in vitro inhibitory activity was investigated with the agar overlay technique, and the minimal inhibitory and bactericidal concentrations were determined. Using the bactericidal concentration, the antimicrobial actions of the inhibitors were investigated. In the present study, multiple techniques were utilized, including scanning electron microscopy (SEM), general structural analysis and differential gene expression analysis. The results obtained from SEM and bactericidal analysis indicated a notable observation; the pentamidine and α‑amylase treatment destroyed the structure of the bacterial cell membranes, which led to cell death. These results were used to further explore these inhibitors and the mechanisms by which they act. Downregulated expression levels were observed for a number of genes coding for hemagglutinins and gingipains, and various genes involved in hemin uptake, chromosome replication and energy production. However, the expression levels of genes associated with iron storage and oxidative stress were upregulated by α‑amylase and pentamidine. A greater effect was noted in response to pentamidine treatment. The results of the present study demonstrate promising therapeutic potential for α‑amylases and pentamidine. These molecules have the potential to be used to develop novel drugs and broaden the availability of pharmacological tools for the attenuation of oral infections caused by Porphyromonas gingivalis.

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1	Darveau RP, Tanner A and Page RC: The microbial challenge in periodontitis. Periodontol 2000. 14:12–32. 1997. View Article : Google Scholar : PubMed/NCBI
2	Eke PI, Dye BA, Wei L, et al CDC Periodontal Disease Surveillance Workgroup: Prevalence of periodontitis in adults in the United States: 2009 and 2010. J Dent Res. 91:914–920. 2012. View Article : Google Scholar : PubMed/NCBI
3	Hutter G, Schlagenhauf U, Valenza G, et al: Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens. Microbiology. 149:67–75. 2003. View Article : Google Scholar : PubMed/NCBI
4	Hajishengallis G, Liang S, Payne MA, et al: Low-abundance biofilm species orchestrates inflammatory periodontal disease through the commensal microbiota and complement. Cell Host Microbe. 10:497–506. 2011. View Article : Google Scholar : PubMed/NCBI
5	Holt SC, Kesavalu L, Walker S and Genco CA: Virulence factors of Porphyromonas gingivalis. Periodontol 2000. 20:168–238. 1999. View Article : Google Scholar : PubMed/NCBI
6	Lamont RJ and Jenkinson HF: Subgingival colonization by Porphyromonas gingivalis. Oral Microbiol Immunol. 15:341–349. 2000. View Article : Google Scholar
7	Gorr SU: Antimicrobial peptides in periodontal innate defense. Front Oral Biol. 15:84–98. 2012.
8	Gupta R, Gigras P, Mohapatra H, et al: Microbial α-amylases: a biotechnological perspective. Process Biochem. 38:1599–1616. 2003. View Article : Google Scholar
9	Rajagopalan G and Krishnan C: Alpha-amylase production from catabolite derepressed Bacillus subtilis KCC103 utilizing sugarcane bagasse hydrolysate. Bioresour Technol. 99:3044–3050. 2008. View Article : Google Scholar
10	Pandey A, Nigam P, Soccol CR, et al: Advances in microbial amylases. Biotechnol Appl Biochem. 31(Pt 2): 135–152. 2000. View Article : Google Scholar : PubMed/NCBI
11	Burchmore RJ, Ogbunude PO, Enanga B and Barrett MP: Chemotherapy of human African trypanosomiasis. Curr Pharm Des. 8:256–267. 2002. View Article : Google Scholar : PubMed/NCBI
12	Wilson WD, Tanious F, Mathis A, et al: Antiparasitic compounds that target DNA. Biochimie. 90:999–1014. 2008. View Article : Google Scholar : PubMed/NCBI
13	Pearson RD and Hewlett EL: Pentamidine for the treatment of Pneumocystis carinii pneumonia and other protozoal diseases. Ann Intern Med. 103:782–786. 1985. View Article : Google Scholar : PubMed/NCBI
14	Charpentier TH, Wilder PT, Liriano MA, et al: Divalent metal ion complexes of S100B in the absence and presence of pent-amidine. J Mol Biol. 382:56–73. 2008. View Article : Google Scholar : PubMed/NCBI
15	Cole AM, Weis P and Diamond G: Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. J Biol Chem. 272:12008–12013. 1997. View Article : Google Scholar : PubMed/NCBI
16	Fischer CL, Walters KS, Drake DR, et al: Sphingoid bases are taken up by Escherichia coli and Staphylococcus aureus and induce ultrastructural damage. Skin Pharmacol Physiol. 26:36–44. 2013. View Article : Google Scholar :
17	Bibel DJ, Aly R, Shah S and Shinefield HR: Sphingosines: antimicrobial barriers of the skin. Acta Derm Venereol. 73:407–411. 1993.PubMed/NCBI
18	Khulusi S, Ahmed HA, Patel P, Mendall MA and Northfield TC: The effects of unsaturated fatty acids on Helicobacter pylori in vitro. J Med Microbiol. 42:276–282. 1995. View Article : Google Scholar : PubMed/NCBI
19	Fischer CL, Drake DR, Dawson DV, et al: Antibacterial activity of sphingoid bases and fatty acids against Gram-positive and Gram-negative bacteria. Antimicrob Agents Chemother. 56:1157–1161. 2012. View Article : Google Scholar :
20	Smalley JW, Birss AJ, Szmigielski B and Potempa J: The HA2 haemagglutinin domain of the lysine-specific gingipain (Kgp) of Porphyromonas gingivalis promotes micro-oxo bishaem formation from monomeric iron(III) protoporphyrin IX. Microbiology. 152:1839–1845. 2006. View Article : Google Scholar : PubMed/NCBI
21	Lewis JP, Dawson JA, Hannis JC, Muddiman D and Macrina FL: Hemoglobinase activity of the lysine gingipain protease (Kgp) of Porphyromonas gingivalis W83. J Bacteriol. 181:4905–4913. 1999.PubMed/NCBI
22	Wright GD: On the road to bacterial cell death. Cell. 130:781–783. 2007. View Article : Google Scholar : PubMed/NCBI
23	Asakura Y and Kobayashi I: From damaged genome to cell surface: transcriptome changes during bacterial cell death triggered by loss of a restriction-modification gene complex. Nucleic Acids Res. 37:3021–3031. 2009. View Article : Google Scholar : PubMed/NCBI