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Article

Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line

  • Authors:
    • Shaorong Han
    • Xiaoyan Lv
    • Yanming Wang
    • Hai Gong
    • Cong Zhang
    • Anna Tong
    • Baoyi Zhang
    • Hui Yao
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Jinan Military General Hospital, Jinan, Shandong 250031, P.R. China
  • Pages: 2239-2246
    |
    Published online on: April 16, 2015
       https://doi.org/10.3892/mmr.2015.3625
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Abstract

Peroxisome proliferator-activated receptor-γ (PPAR-γ) is important in tumor differentiation, proliferation and apoptosis. However, the effect and mechanism of PPAR‑γ on the promotion of cisplatin sensitivity in glioma cells remain to be elucidated. The present study established cisplatin‑resistant U‑87 MG/CDDP cell lines and U‑87 MG/CDDP cell lines overexpressing PPAR‑γ. With upregulated expression of PPAR‑γ, the sensitivity of cancer cells to cisplatin was increased. Flow cytometry revealed that the intracellular content of rhodamine‑123 was increased, expression of P‑glycoprotein was downregulated, cell cycle was arrested in G0/G1 phase, apoptosis and oxidative stress was increased, levels of intracellular thymidylate synthase, glutathione and transforming growth factor‑β1 were decreased, expression levels of multidrug resistance related gene (MDR), multidrug resistance‑associated protein and glutothionine S‑transferase‑π were downregulated, expression levels of cell proliferation and apoptosis associated genes, including survivin and B‑cell lymphoma‑2, were downregulated, p53, p21 and caspase‑3/8 were significantly upregulated, phosphorylation of extracellular signal‑regulated kinase and small mothers against decapentaplegic 2 were downregulated, and the transcriptional activities of Twist and nuclear factor (erythroid‑derived 2)‑like 2 were significantly reduced. The results suggested that upregulation of PPAR‑γ in the U‑87 MG/DDP cells increased cisplatin sensitivity, and the underlying mechanisms included the regulation of MDR and apoptosis associated genes, which increased the intracellular accumulation of the drug, inhibited cell proliferation and promoted cell apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Han S, Lv X, Wang Y, Gong H, Zhang C, Tong A, Zhang B and Yao H: Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line. Mol Med Rep 12: 2239-2246, 2015.
APA
Han, S., Lv, X., Wang, Y., Gong, H., Zhang, C., Tong, A. ... Yao, H. (2015). Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line. Molecular Medicine Reports, 12, 2239-2246. https://doi.org/10.3892/mmr.2015.3625
MLA
Han, S., Lv, X., Wang, Y., Gong, H., Zhang, C., Tong, A., Zhang, B., Yao, H."Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line". Molecular Medicine Reports 12.2 (2015): 2239-2246.
Chicago
Han, S., Lv, X., Wang, Y., Gong, H., Zhang, C., Tong, A., Zhang, B., Yao, H."Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line". Molecular Medicine Reports 12, no. 2 (2015): 2239-2246. https://doi.org/10.3892/mmr.2015.3625
Copy and paste a formatted citation
x
Spandidos Publications style
Han S, Lv X, Wang Y, Gong H, Zhang C, Tong A, Zhang B and Yao H: Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line. Mol Med Rep 12: 2239-2246, 2015.
APA
Han, S., Lv, X., Wang, Y., Gong, H., Zhang, C., Tong, A. ... Yao, H. (2015). Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line. Molecular Medicine Reports, 12, 2239-2246. https://doi.org/10.3892/mmr.2015.3625
MLA
Han, S., Lv, X., Wang, Y., Gong, H., Zhang, C., Tong, A., Zhang, B., Yao, H."Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line". Molecular Medicine Reports 12.2 (2015): 2239-2246.
Chicago
Han, S., Lv, X., Wang, Y., Gong, H., Zhang, C., Tong, A., Zhang, B., Yao, H."Effect and mechanism of peroxisome proliferator-activated receptor-γ on the drug resistance of the U-87 MG/CDDP human malignant glioma cell line". Molecular Medicine Reports 12, no. 2 (2015): 2239-2246. https://doi.org/10.3892/mmr.2015.3625
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