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Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo

  • Authors:
    • Chong‑Zhi Bai
    • Ma‑Li Feng
    • Xu‑Liang Hao
    • Zhi‑Juan Zhao
    • Yu‑Ying Li
    • Zhuan‑Hua Wang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Chemical Biology and Molecular Engineering of the Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, Shanxi 030006, P.R. China, Chinese Medicine Hospital of Shanxi Province, Taiyuan, Shanxi 030012, P.R. China
    Copyright: © Bai et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1777-1782
    |
    Published online on: April 20, 2015
       https://doi.org/10.3892/mmr.2015.3649
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Abstract

Native buckwheat, a common component of food products and medicine, has been observed to inhibit cancer cell proliferation in vitro. The aim of the present study was to evaluate the in vitro and in vivo anti-tumoral effects of recombinant buckwheat trypsin inhibitor (rBTI) on hepatic cancer cells and the mechanism of apoptosis involved. Apoptosis in the H22 cell line induced by rBTI was identified using MTT assays, DNA electrophoresis, flow cytometry, morphological observation of the nuclei, measurement of cytochrome C and assessment of caspase activation. It was identified that rBTI decreases cell viability by inducing apoptosis, as evidenced by the formation of apoptotic bodies and DNA fragmentation. rBTI‑induced apoptosis occurred in association with mitochondrial dysfunction, leading to the release of cytochrome C from the mitochondria to the cytosol, as well as the activation of caspase‑3, ‑8 and ‑9. In conclusion, the results of the present study suggested that rBTI specifically inhibited the growth of the H22 hepatic carcinoma cell line in vitro and in vivo in a concentration‑dependent and time‑dependent manner, while there were minimal effects on the 7702 normal liver cell line. In addition, rBTI‑induced apoptosis in H22 cells was, at least in part, mediated by a mitochondrial pathway via caspase‑9.
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1 

Li CH, Kobayasshi K, Yoshida Y and Ohsawa R: Genetic analyses of agronomic traits in Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.). Breed Sci. 62:303–309. 2012. View Article : Google Scholar

2 

Pomeranz Y and Robbins GS: Amino acid composition of buckwheat protein. J Agric Food Chem. 20:270–274. 1972. View Article : Google Scholar

3 

Ikeda K and Kishida M: Digestibility of proteins in buckwheat seed. Fagopyrum. 13:21–24. 1993.

4 

Zhang Z, Li YY, Shi XR, et al: Gene cloning and sequencing of common buckwheat trypsin inhibitor. Acta Botanica Boreali-Occidentalia Sinica. 25:46–51. 2005.

5 

Htay HH, Tsubouchi R, Haneda M, Murakami K and Yoshino M: Induction of apoptosis of HL60 cells by gallic acid derivatives. Biomed Res. 23:127–134. 2002. View Article : Google Scholar

6 

Gardner PR: Aconitase: Sensitive target and measure of superoxide. Methods Enzymol. 349:9–23. 2002.PubMed/NCBI

7 

Gladysheva IP, Moroz NA, Karmakova TA, Nemtsova ER, Yakubovskaya RI and Larionova NI: Immunoconjugates of soybean Bowman-Birk protease inhibitor as targeted antitumor polymeric agents. J Drug Target. 9:303–316. 2001. View Article : Google Scholar

8 

Marschütz MK and Bernkop-Schnürch A: Oral peptide drug delivery: Polymer-inhibitor conjugates protecting insulin from enzymatic degradation in vitro. Biomaterials. 21:1499–1507. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Malkowicz SB, Liu SP, Broderick GA, Wein AJ, Kennedy AR and Levin RM: Effect of the Bowman-Birk inhibitor (a soy protein) on in vitro bladder neck/urethral and penile corporal smooth muscle activity. Neurourol Urodyn. 22:54–57. 2003. View Article : Google Scholar

10 

Supuran CT, Casini A and Scozzafava A: Protease inhibitors of the sulfonamide type: Anticancer, antiinflammatory, and antiviral agents. Med Res Rev. 23:535–558. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Birk Y: The Bowman-Birk inhibitor. Trypsin- and chymotrypsin-inhibitor from soybeans. Int J Pept Protein Res. 25:113–131. 1985. View Article : Google Scholar : PubMed/NCBI

12 

Yavelow J, Finlay TH, Kennedy AR and Troll W: Bowman-Birk soybean protease inhibitor as an anticarcinogen. Cancer Res. 43(Suppl): 2454s–2459s. 1983.PubMed/NCBI

13 

Cryns V and Yuan J: Proteases to die for. Genes Dev. 12:1551–1570. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Hu W and Kavanagh JJ: Anticancer therapy targeting the apoptotic pathway. Lancet Oncol. 4:721–729. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Dias N and Bailly C: Drugs targeting mitochondrial functions to control tumor cell growth. Biochem Pharmacol. 70:1–12. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Schulze-Osthoff K, Ferrari D, Los M, Wesselborg S and Peter ME: Apoptosis signaling by death receptors. Eur J Biochem. 254:439–459. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Rao RV, Ellerby HM and Bredesen DE: Coupling endoplasmic reticulum stress to the cell death program. Cell Death Differ. 11:372–380. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Budihardjo I, Oliver H, Lutter M, Luo X and Wang X: Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol. 15:269–290. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Liu X, Kim CN, Yang J, Jemmerson R and Wang X: Induction of apoptotic program in cell-free extracts: Requirement for dATP and cytochrome c. Cell. 86:147–157. 1996. View Article : Google Scholar : PubMed/NCBI

20 

Thorburn A: Death receptor-induced cell killing. Cell Signal. 16:139–144. 2004. View Article : Google Scholar

21 

Lacour S, Micheau O, Hammann A, Drouineaud V, Tschopp J, Solary E and Dimanche-Boitrel MT: Chemotherapy enhances TNF-related apoptosis-inducing ligand DISC assembly in HT29 human colon cancer cells. Oncogene. 22:1807–1816. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Zhang Z, Li Y, Li C, Yuan J and Wang Z: Expression of a buckwheat trypsin inhibitor gene in Escherichia coli and its effect on multiple myeloma IM-9 cell proliferation. Acta Biochim Biophys Sin (Shanghai). 39:701–707. 2007. View Article : Google Scholar

23 

Wang ZH, Gao L, Li YY, Zhang Z, Yuan JM, Wang HW, Zhang L and Zhu L: Induction of apoptosis by buckwheat trypsin inhibitor in chronic myeloid leukemia K562 cells. Biol Pharm Bull. 30:783–786. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Park SS and Ohba H: Suppressive activity of protease inhibitors from buckwheat seeds against human T-acute lymphoblastic leukemia cell lines. Appl Biochem Biotechnol. 117:65–74. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Stennicke HR and Salvesen GS: Properties of the caspases. Biochim Biophys Acta. 1387:17–31. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Hengartner MO: The biochemistry of apoptosis. Nature. 407:770–776. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Park JW, Choi YJ, Suh SI, Baek WK, Suh MH, Jin IN, Min DS, Woo JH, Chang JS, Passaniti A, et al: Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity. Carcinogenesis. 22:1633–1639. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Kaufmann SH and Hengartner MO: Programmed cell death: Alive and well in the new millennium. Trends Cell Biol. 11:526–534. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES and Wang X: Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell. 91:479–489. 1997. View Article : Google Scholar : PubMed/NCBI

30 

Narayanan S, Surolia A and Karande AA: Ribosome-inactivating protein and apoptosis: Abrin causes cell death via mitochondrial pathway in Jurkat cells. Biochem J. 377:233–240. 2004. View Article : Google Scholar

31 

Smith EM and Jayson GC: The current and future management of malignant ascites. Clin Oncol (R Coll Radiol). 15:59–72. 2003. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Bai CZ, Feng ML, Hao XL, Zhao ZJ, Li YY and Wang ZH: Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo. Mol Med Rep 12: 1777-1782, 2015.
APA
Bai, C., Feng, M., Hao, X., Zhao, Z., Li, Y., & Wang, Z. (2015). Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo. Molecular Medicine Reports, 12, 1777-1782. https://doi.org/10.3892/mmr.2015.3649
MLA
Bai, C., Feng, M., Hao, X., Zhao, Z., Li, Y., Wang, Z."Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo". Molecular Medicine Reports 12.2 (2015): 1777-1782.
Chicago
Bai, C., Feng, M., Hao, X., Zhao, Z., Li, Y., Wang, Z."Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo". Molecular Medicine Reports 12, no. 2 (2015): 1777-1782. https://doi.org/10.3892/mmr.2015.3649
Copy and paste a formatted citation
x
Spandidos Publications style
Bai CZ, Feng ML, Hao XL, Zhao ZJ, Li YY and Wang ZH: Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo. Mol Med Rep 12: 1777-1782, 2015.
APA
Bai, C., Feng, M., Hao, X., Zhao, Z., Li, Y., & Wang, Z. (2015). Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo. Molecular Medicine Reports, 12, 1777-1782. https://doi.org/10.3892/mmr.2015.3649
MLA
Bai, C., Feng, M., Hao, X., Zhao, Z., Li, Y., Wang, Z."Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo". Molecular Medicine Reports 12.2 (2015): 1777-1782.
Chicago
Bai, C., Feng, M., Hao, X., Zhao, Z., Li, Y., Wang, Z."Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo". Molecular Medicine Reports 12, no. 2 (2015): 1777-1782. https://doi.org/10.3892/mmr.2015.3649
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