Hypoglycemic effects of aqueous persimmon leaf extract in a murine model of diabetes

Bae,Ui‑Jin; Park,Soo‑Hyun; Jung,Su‑Young; Park,Byung‑Hyun; Chae,Soo‑Wan

doi:10.3892/mmr.2015.3766

August-2015 Volume 12 Issue 2

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August-2015 Volume 12 Issue 2

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Article Open Access

Hypoglycemic effects of aqueous persimmon leaf extract in a murine model of diabetes

Authors:
- Ui‑Jin Bae
- Soo‑Hyun Park
- Su‑Young Jung
- Byung‑Hyun Park
- Soo‑Wan Chae
View Affiliations / Copyright

Affiliations: Department of Biochemistry, Chonbuk National University Medical School, Deokjin‑gu, Jeonju, Jeonbuk 561‑756, Republic of Korea, Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Deokjin‑gu, Jeonju, Jeonbuk 561‑712, Republic of Korea

Copyright: © Bae et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 2547-2554
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Published online on: May 8, 2015

https://doi.org/10.3892/mmr.2015.3766
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Abstract

Previously, powdered persimmon leaves have been reported to have glucose‑ and lipid‑lowering effects in diabetic (db/db) mice. As persimmon leaf is commonly consumed as tea, an aqueous extract of persimmon leaves (PLE) was prepared and its anti‑diabetic efficacy was investigated. In the present study, PLE was tested for its inhibitory activity on α‑glucosidase in vitro. An oral maltose tolerance test was performed in diabetic mice. Next, the acute effect of PLE was examined in streptozotocin‑induced diabetic mice. Last, the long‑term effect of PLE supplementation was assessed in db/db after eight weeks. An oral glucose tolerance test, biochemical parameters, as well as histological analyses of liver and pancreas were evaluated at the end of the study. PLE inhibited α‑glucosidase activity and increased antioxidant capacity. Streptozotocin‑induced diabetic mice pre‑treated with PLE displayed hypoglycemic activity. Daily oral supplementation with PLE for eight weeks reduced body weight gain without affecting food intake, enhanced the glucose tolerance during the oral glucose tolerance test (OGTT), improved blood lipid parameters, suppressed fat accumulation in the liver and maintained islet structure in db/db mice. Further mechanistic study showed that PLE protected pancreatic islets from glucotoxicity. In conclusion, the results of the present study indicated that PLE exhibits considerable anti‑diabetic effects through α‑glucosidase inhibition and through the maintenance of functional β‑cells. These results provided a rationale for the use of persimmon leaf tea for the maintenance of normal blood glucose levels in diabetic patients.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Hypoglycemic effects of aqueous persimmon leaf extract in a murine model of diabetes

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