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Article

Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis

  • Authors:
    • Di‑Fei Wang
    • Hui‑Jing Yang
    • Jian‑Qiu Gu
    • Yan‑Li Cao
    • Xin Meng
    • Xiao‑Li Wang
    • Yi‑Chen Lin
    • Ming Gao
  • View Affiliations / Copyright

    Affiliations: Department of Geriatric Endocrinology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 2695-2700
    |
    Published online on: May 12, 2015
       https://doi.org/10.3892/mmr.2015.3771
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Abstract

In the present study, a glucosamine-induced model of insulin-resistant skeletal muscle cells was established in order to investigate the effect of inhibition of phosphatase and tensin homolog (PTEN)/5'-adenosine monophosphate-activated protein kinase (AMPK) on these cells. The glucosamine‑induced insulin‑resistant skeletal muscle cells were produced and the rate of glucose uptake was measured using the glucose oxidase‑peroxidase method. The expression levels of PTEN and phosphorylated PTEN (p‑PTEN) were assessed using western blotting. Glucose transporter 4 (GLUT4) translocation was detected by immunofluorescence. Cell apoptosis was evaluated using flow cytometry. Following insulin stimulation, the rate of glucose uptake was significantly reduced in the cells with glucosamine‑induced insulin‑resistance in comparison with those in the control group. The expression and translocation of GLUT4 were reduced in the insulin‑resistant muscle cells. By contrast, the expression of PTEN and p‑PTEN as well as apoptosis were significantly increased. Following treatment with bisperoxopicolinatooxovanadate (BPV) or metformin in the insulin‑resistant skeletal muscle cells, there was an increase in the rate of glucose uptake, an increase in GLUT4 expression and its translocation, a reduction in the expression of PTEN and p‑PTEN, and a decrease in cell apoptosis compared with untreated insulin‑resistant cells. Glucosamine may be used to produce an effective model of insulin‑resistant skeletal muscle cells. Cells with glucosamine‑induced insulin‑resistance exhibited a reduced expression of GLUT4 and dysfunction in GLUT4 translocation, as well as increased activation of PTEN and increased cell apoptosis. Inhibition of PTEN or its upstream regulator, AMPK, protects glucosamine‑induced insulin-resistant skeletal muscle cells from apoptosis.
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1 

McCarthy AM, Spisak KO, Brozinick JT and Elmendorf JS: Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport. Am J Physiol Cell Physiol. 291:C860–C868. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Habegger KM, Penque BA, Sealls W, Tackett L, Bell LN, Blue EK, Gallagher PJ, Sturek M, Alloosh MA, Steinberg HO, Considine RV and Elmendorf JS: Fat-induced membrane cholesterol accrual provokes cortical filamentous actin destabilisation and glucose transport dysfunction in skeletal muscle. Diabetologia. 55:457–467. 2012. View Article : Google Scholar

3 

Rando TA and Blau HM: Primary mouse myoblast purification, characterization and transplantation for cell-mediated gene therapy. J Cell Biol. 125:1275–1287. 1994. View Article : Google Scholar : PubMed/NCBI

4 

Dostrovsky NR, Towheed TE, Hudson RW and Anastassiades TP: The effect of glucosamine on glucose metabolism in humans: a systematic review of the literature. Osteoarthritis Cartilage. 19:375–380. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Chien CS, Cheng SC, Wu HT, Tsao CW and Cheng JT: Insulin resistance induced by glucosamine in fructose-fed rats. Horm Metab Res. 41:542–547. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Kang L, Chen CH, Cheng YC, Chang CH, Lee CT, Chang JK, Cheng JT and Chang FM: Glucosamine-induced insulin resistance in ovariectomized rats is relevant to decreasing the expression of glucose transport protein subtype 4 in the skeletal muscle and in increasing the size of pancreatic islets. Menopause. 19:496–502. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Sakai K and Clemmons DR: Glucosamine induces resistance to insulin-like growth factor I (IGF-I) and insulin in Hep G2 cell cultures: biological significance of IGF-I/insulin hybrid receptors. Endocrinology. 144:2388–2395. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Lee JO, Lee SK, Kim JH, Kim N, You GY, Moon JW, Kim SJ, Park SH and Kim HS: Metformin regulates glucose transporter 4 (GLUT4) translocation through AMP-activated protein kinase (AMPK)-Mediated Cbl/CAP Signaling in 3T3-L1 Preadipocytecells. J Biol Chem. 287:44121–44129. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Meshkani R and Adeli K: Hepatic insulin resistance, metabolic syndrome and cardiovascular disease. Clin Biochem. 42:1331–1346. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Joshi A and Ellenson LH: Adenovirus mediated homozygous endometrial epithelial Pten deletion results in aggressive endometrial carcinoma. Exp Cell Res. 317:1580–1589. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang SI, Puc J, Miliaresis C, Rodgers L, McCombie R, et al: PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast and prostate cancer. Science. 275:1943–1947. 1997. View Article : Google Scholar : PubMed/NCBI

12 

Nyåkern M, Tazzari PL, Finelli C, Bosi C, Follo MY, Grafone T, Piccaluga PP, Martinelli G, Cocco L and Martelli AM: Frequent elevation of Akt kinase phosphorylation in blood marrow and peripheral blood mononuclear cells from high-risk myelodysplastic syndrome patients. Leukemia. 20:230–238. 2006. View Article : Google Scholar

13 

Kim SA and Choi HC: Metformin inhibits inflammatory response via AMPK-PTEN pathway in vascular smooth muscle cells. Biochem Biophys Res Commun. 425:866–872. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Dimchev GA, Al-Shanti N and Stewart CE: Phospho-tyrosine phosphatase inhibitor Bpv (Hopic) enhances C2C12 myoblast migration in vitro. Requirement of PI3K/AKT and MAPK/ERK pathways. J Muscle Res Cell Motil. 34:125–136. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Li B, Takeda T, Tsuiji K, Kondo A, Kitamura M, Wong TF and Yaegashi N: The antidiabetic drug metformin inhibits uterine leiomyoma cell proliferation via an AMP-activated protein kinase signaling pathway. Gynecol Endocrinol. 29:87–90. 2013. View Article : Google Scholar

16 

Zorzano A, Fandos C and Palacin M: Role of plasma membrane transporters in muscle metabolism. Biochem J. 349:667–688. 2000.PubMed/NCBI

17 

Alkhateeb H, Chabowski C, Glatz JFC, Luiken JFA and Bonen A: Two phases of palmitate-induced insulin resistance in skeletal muscle: impaired GLUT4 translocation is followed by a reduced GLUT4 intrinsic activity. Am J Physiol Endocrinol Metab. 293:E783–E793. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Zierath JR, Krook A and Wallberg-Henriksson H: Insulin action in skeletal muscle from patients with NIDDM. Mol Cell Biochem. 182:153–160. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Qu W, Zhao L, Peng X, Yang X, Ying C, Hao L and Sun X: Biphasic effects of chronic ethanol exposure on insulin-stimulated glucose uptake in primary cultured rat skeletal muscle cells: role of the Akt pathway and GLUT4. Diabetes Metab Res Rev. 47–53. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Zhou QL, Jiang Z, Mabardy AS, Del Campo CM, Lambright DG, Holik J, Fogarty KE, Straubhaar J, Nicoloro S, Chawla A and Czech MP: A novel pleckstrin homology domain-containing protein enhances insulin-stimulated Akt phosphorylation and GLUT4 translocation in adipocytes. J Biol Chem. 285:27581–27589. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Sugimoto N, Miwa S, Ohno-Shosaku T, Tsuchiya H, Hitomi Y, Nakamura H, Tomita K, Yachie A and Koizumi S: Activation of tumor suppressor protein PTEN and induction of apoptosis are involved in cAMP-mediated inhibition of cell number in B92 glial cells. Neurosci Lett. 497:55–59. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang DF, Yang HJ, Gu JQ, Cao YL, Meng X, Wang XL, Lin YC and Gao M: Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis. Mol Med Rep 12: 2695-2700, 2015.
APA
Wang, D., Yang, H., Gu, J., Cao, Y., Meng, X., Wang, X. ... Gao, M. (2015). Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis. Molecular Medicine Reports, 12, 2695-2700. https://doi.org/10.3892/mmr.2015.3771
MLA
Wang, D., Yang, H., Gu, J., Cao, Y., Meng, X., Wang, X., Lin, Y., Gao, M."Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis". Molecular Medicine Reports 12.2 (2015): 2695-2700.
Chicago
Wang, D., Yang, H., Gu, J., Cao, Y., Meng, X., Wang, X., Lin, Y., Gao, M."Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis". Molecular Medicine Reports 12, no. 2 (2015): 2695-2700. https://doi.org/10.3892/mmr.2015.3771
Copy and paste a formatted citation
x
Spandidos Publications style
Wang DF, Yang HJ, Gu JQ, Cao YL, Meng X, Wang XL, Lin YC and Gao M: Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis. Mol Med Rep 12: 2695-2700, 2015.
APA
Wang, D., Yang, H., Gu, J., Cao, Y., Meng, X., Wang, X. ... Gao, M. (2015). Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis. Molecular Medicine Reports, 12, 2695-2700. https://doi.org/10.3892/mmr.2015.3771
MLA
Wang, D., Yang, H., Gu, J., Cao, Y., Meng, X., Wang, X., Lin, Y., Gao, M."Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis". Molecular Medicine Reports 12.2 (2015): 2695-2700.
Chicago
Wang, D., Yang, H., Gu, J., Cao, Y., Meng, X., Wang, X., Lin, Y., Gao, M."Suppression of phosphatase and tensin homolog protects insulin-resistant cells from apoptosis". Molecular Medicine Reports 12, no. 2 (2015): 2695-2700. https://doi.org/10.3892/mmr.2015.3771
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