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Article

CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts

  • Authors:
    • Kang Li
    • Zeng Dan
    • Yuqiang Nie
    • Xuejun Hu
    • Luobu Gesang
    • Zhaxi Bianba
    • Yongge Ze
    • Cuomu Ciren
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, People's Hospital of Tibet Autonomous Region, Lhasa, Tibet Autonomous Region 850000, P.R. China, Department of Gastroenterology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China, Department of Oncology, People's Hospital of Tibet Autonomous Region, Lhasa, Tibet Autonomous Region 850000, P.R. China
  • Pages: 4332-4339
    |
    Published online on: June 12, 2015
       https://doi.org/10.3892/mmr.2015.3924
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Abstract

The present study examined the role of CD14 in the regulation of lipopolysaccharide (LPS)-induced effects on gastric cancer cells. MGC‑803 cells were stably transfected with CD14 short hairpin (sh)RNA and treated with LPS, followed by assessment of cell proliferation, apoptosis and gene expression using a cell counting kit‑8 assay, flow cytometry, reverse transcription‑polymerase chain reaction and western blot analysis, respectively. The cells subjected to CD14 knockdown were treated with 10 g/ml LPS and injected into nude mice to form tumor xenografts. CD14 shRNA‑transfected MGC‑803 cells did not exhibit any significant changes in cell viability compared with the control cells (P>0.05), but cell viability was markedly increased in the wild‑type (WT) + LPS group (P<0.05). In contrast to the WT + LPS group, the cell viability of the sh‑CD14 + LPS group was markedly decreased (P<0.05). In addition, compared with those in the controls, the level of sh‑CD14 cell apoptosis did not change significantly; however, it was markedly reduced in the LPS group. Compared with that in the WT + LPS group, the rate of apoptosis in the sh‑CD14 + LPS group increased to a certain extent, while it remained lower in the control group. In addition, compared with that in the control, the expression of tumor necrosis factor‑α, interleukin (IL)‑1, IL‑6 and IL‑12, and human β‑defensin 2 was significantly increased in the WT + LPS group, while, compared with that in the WT + LPS group, the expression of these genes was markedly reduced in the sh‑CD14 + LPS group (P<0.05). The nude mouse experiments further confirmed the in vitro data, including the finding that LPS promoted the growth of xenografts, but knockdown of CD14 expression reduced the response of tumor cells to LPS treatment. In conclusion, LPS induced cell viability and the release of inflammatory cytokines, but inhibited gastric cancer cell apoptosis. Knockdown of CD14 expression had no significant effect on gastric cancer malignancy, but mediated LPS signal transduction.
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1 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Parkin DM: The global health burden of infection-associated cancers in the year 2002. Int J Cancer. 118:3030–3044. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Herrera V and Parsonnet J: Helicobacter pylori and gastric adenocarcinoma. Clin Microbiol Infect. 15:971–976. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Pugin J, Heumann ID, Tomasz A, et al: CD14 is a pattern recognition receptor. Immunity. 1:509–516. 1994. View Article : Google Scholar : PubMed/NCBI

5 

Wright SD, Ramos RA, Tobias PS, Ulevitch RJ and Mathison JC: CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249:1431–1433. 1990. View Article : Google Scholar : PubMed/NCBI

6 

Li K, Dan Z, Hu X, et al: CD14 overexpression upregulates TNF-alpha-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells. Mol Cell Biochem. 376:137–143. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Gadducci A, Ferdeghini M, Castellani C, et al: Serum levels of tumor necrosis factor (TNF), soluble receptors for TNF (55- and 75-kDa sTNFr) and soluble CD14 (sCD14) in epithelial ovarian cancer. Gynecol Oncol. 58:184–188. 1995. View Article : Google Scholar : PubMed/NCBI

8 

Kanczkowski W, Tymoszuk P, Ehrhart-Bornstein M, Wirth MP, Zacharowski K and Bornstein SR: Abrogation of TLR4 and CD14 expression and signaling in human adrenocortical tumors. J Clin Endocrinol Metab. 95:E421–E429. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Li K, Dan Z, Hu X, Gesang L, Ze Y and Bianba Z: CD14 regulates gastric cancer cell epithelialmesenchymal transition and invasion in vitro. Oncol Rep. 30:2725–2732. 2013.PubMed/NCBI

10 

Peek RM Jr, Fiske C and Wilson KT: Role of innate immunity in Helicobacter pylori-induced gastric malignancy. Physiol Rev. 90:831–858. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Polk DB and Peek RM Jr: Helicobacter pylori: gastric cancer and beyond. Nat Rev Cancer. 10:403–414. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Correa P and Piazuelo MB: Helicobacter pylori infection and gastric adenocarcinoma. US Gastroenterol Hepatol Rev. 7:59–64. 2011.PubMed/NCBI

13 

Blaser MJ and Kirschner D: The equilibria that allow bacterial persistence in human hosts. Nature. 449:843–849. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Schmausser B, Andrulis M, Endrich S, et al: Expression and subcellular distribution of toll-like receptors TLR4, TLR5 and TLR9 on the gastric epithelium in Helicobacter pylori infection. Clin Exp Immunol. 136:521–526. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Schmausser B, Andrulis M, Endrich S, Muller-Hermelink HK and Eck M: Toll-like receptors TLR4, TLR5 and TLR9 on gastric carcinoma cells: an implication for interaction with Helicobacter pylori. Int J Med Microbiol. 295:179–185. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Zhao D, Sun T, Zhang X, et al: Role of CD14 promoter polymorphisms in Helicobacter pylori infection-related gastric carcinoma. Clin Cancer Res. 13:2362–2368. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Grandel U, Banat A and Savai R: Effect of endotoxin on COX-2 dependent proliferation of NSCLC cells: Role of CD14, TLRs and EGFR signaling. J Clin Oncol (Meeting Abstracts). e221902009.

18 

Hattar K, Savai R, Subtil FS, et al: Endotoxin induces proliferation of NSCLC in vitro and in vivo: role of COX-2 and EGFR activation. Cancer Immunol Immunother. 62:309–320. 2013. View Article : Google Scholar :

19 

He W, Liu Q, Wang L, Chen W, Li N and Cao X: TLR4 signaling promotes immune escape of human lung cancer cells by inducing immunosuppressive cytokines and apoptosis resistance. Mol Immunol. 44:2850–2859. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Chan KL, Wong KF and Luk JM: Role of LPS/CD14/TLR4-mediated inflammation in necrotizing enterocolitis: pathogenesis and therapeutic implications. World J Gastroenterol. 15:4745–4752. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Baumann CL, Aspalter IM, Sharif O, et al: CD14 is a coreceptor of Toll-like receptors 7 and 9. J Exp Med. 207:2689–2701. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Wang JH, Manning BJ, Wu QD, Blankson S, Bouchier-Hayes D and Redmond HP: Endotoxin/lipopolysaccharide activates NF-kappaB and enhances tumor cell adhesion and invasion through a beta 1 integrin-dependent mechanism. J Immunol. 170:795–804. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Molteni M, Marabella D, Orlandi C and Rossetti C: Melanoma cell lines are responsive in vitro to lipopolysaccharide and express TLR-4. Cancer Lett. 235:75–83. 2006. View Article : Google Scholar

24 

Kelly MG, Alvero AB, Chen R, et al: TLR-4 signaling promotes tumor growth and paclitaxel chemoresistance in ovarian cancer. Cancer Res. 66:3859–3868. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Bauer B, Wex T, Kuester D, Meyer T and Malfertheiner P: Differential expression of human beta defensin 2 and 3 in gastric mucosa of Helicobacter pylori-infected individuals. Helicobacter. 18:6–12. 2013. View Article : Google Scholar

26 

Moran AP: The role of endotoxin in infection: Helicobacter pylori and Campylobacter jejuni. Subcell Biochem. 53:209–240. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Xie C, Kang J, Li Z, et al: The açaí flavonoid velutin is a potent anti-inflammatory agent: blockade of LPS-mediated TNF-alpha and IL-6 production through inhibiting NF-kappaB activation and MAPK pathway. J Nutr Biochem. 23:1184–1191. 2012. View Article : Google Scholar

28 

Dolcet X, Llobet D, Pallares J and Matias-Guiu X: NF-kB in development and progression of human cancer. Virchows Arch. 446:475–482. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Akira S and Takeda K: Toll-like receptor signalling. Nat Rev Immunol. 4:499–511. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Underhill DM and Ozinsky A: Toll-like receptors: key mediators of microbe detection. Curr Opin Immunol. 14:103–110. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li K, Dan Z, Nie Y, Hu X, Gesang L, Bianba Z, Ze Y and Ciren C: CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts. Mol Med Rep 12: 4332-4339, 2015.
APA
Li, K., Dan, Z., Nie, Y., Hu, X., Gesang, L., Bianba, Z. ... Ciren, C. (2015). CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts. Molecular Medicine Reports, 12, 4332-4339. https://doi.org/10.3892/mmr.2015.3924
MLA
Li, K., Dan, Z., Nie, Y., Hu, X., Gesang, L., Bianba, Z., Ze, Y., Ciren, C."CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts". Molecular Medicine Reports 12.3 (2015): 4332-4339.
Chicago
Li, K., Dan, Z., Nie, Y., Hu, X., Gesang, L., Bianba, Z., Ze, Y., Ciren, C."CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts". Molecular Medicine Reports 12, no. 3 (2015): 4332-4339. https://doi.org/10.3892/mmr.2015.3924
Copy and paste a formatted citation
x
Spandidos Publications style
Li K, Dan Z, Nie Y, Hu X, Gesang L, Bianba Z, Ze Y and Ciren C: CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts. Mol Med Rep 12: 4332-4339, 2015.
APA
Li, K., Dan, Z., Nie, Y., Hu, X., Gesang, L., Bianba, Z. ... Ciren, C. (2015). CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts. Molecular Medicine Reports, 12, 4332-4339. https://doi.org/10.3892/mmr.2015.3924
MLA
Li, K., Dan, Z., Nie, Y., Hu, X., Gesang, L., Bianba, Z., Ze, Y., Ciren, C."CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts". Molecular Medicine Reports 12.3 (2015): 4332-4339.
Chicago
Li, K., Dan, Z., Nie, Y., Hu, X., Gesang, L., Bianba, Z., Ze, Y., Ciren, C."CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts". Molecular Medicine Reports 12, no. 3 (2015): 4332-4339. https://doi.org/10.3892/mmr.2015.3924
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