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Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells

  • Authors:
    • Song Zhao
    • Feng Niu
    • Chang‑Yan Xu
    • Long Ye
    • Gui‑Bin Bi
    • Lin Chen
    • Ping Gong
    • Gang Tian
    • Tian‑Hong Nie
  • View Affiliations / Copyright

    Affiliations: Department of Spinal Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Medical Records, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 4284-4290
    |
    Published online on: June 15, 2015
       https://doi.org/10.3892/mmr.2015.3933
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Abstract

Integrative analysis of chromatin immunoprecipitation-sequencing (ChIP-seq) data and microarray data was performed to illustrate the effect of Nutlin‑3 on promoter selectivity and transcriptional regulation by the tumor suppressor p53 in U2OS human osteosarcoma cells. Raw data (accession number, GSE46642) were downloaded from Gene Expression Omnibus. Differential analyses were performed using package limma of R software. Gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed for the differentially expressed genes (DEGs) using the Database for Annotation, Visualization and Integration Discovery. Integrative analysis of ChIP‑seq data and microarray data were confirmed with ChIP‑Array. A total of 565 DEGs were identified, including 373 upregulated genes and 192 downregulated genes. Genes involved in the p53 signaling pathway, cell cycle, DNA replication, cytokine‑cytokine receptor interaction and melanoma were markedly over‑represented in the DEGs. A total of 39 DEGs were directly regulated by p53 and two were the transcription factors (TFs), E2F2 and HOXA1. E2F2 regulated 25 DEGs, while HOXA1 regulated one DEG. The cell cycle, p53 signaling pathway, melanoma and pathways involved in cancer were enriched in the direct and indirect target genes. Changes in the p53‑binding pattern induced by Nutlin‑3 were described in the present study, which may advance the understanding of the regulatory network of p53 in osteosarcoma and aid in the development of novel therapies.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao S, Niu F, Xu CY, Ye L, Bi GB, Chen L, Gong P, Tian G and Nie TH: Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells. Mol Med Rep 12: 4284-4290, 2015.
APA
Zhao, S., Niu, F., Xu, C., Ye, L., Bi, G., Chen, L. ... Nie, T. (2015). Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells. Molecular Medicine Reports, 12, 4284-4290. https://doi.org/10.3892/mmr.2015.3933
MLA
Zhao, S., Niu, F., Xu, C., Ye, L., Bi, G., Chen, L., Gong, P., Tian, G., Nie, T."Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells". Molecular Medicine Reports 12.3 (2015): 4284-4290.
Chicago
Zhao, S., Niu, F., Xu, C., Ye, L., Bi, G., Chen, L., Gong, P., Tian, G., Nie, T."Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells". Molecular Medicine Reports 12, no. 3 (2015): 4284-4290. https://doi.org/10.3892/mmr.2015.3933
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao S, Niu F, Xu CY, Ye L, Bi GB, Chen L, Gong P, Tian G and Nie TH: Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells. Mol Med Rep 12: 4284-4290, 2015.
APA
Zhao, S., Niu, F., Xu, C., Ye, L., Bi, G., Chen, L. ... Nie, T. (2015). Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells. Molecular Medicine Reports, 12, 4284-4290. https://doi.org/10.3892/mmr.2015.3933
MLA
Zhao, S., Niu, F., Xu, C., Ye, L., Bi, G., Chen, L., Gong, P., Tian, G., Nie, T."Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells". Molecular Medicine Reports 12.3 (2015): 4284-4290.
Chicago
Zhao, S., Niu, F., Xu, C., Ye, L., Bi, G., Chen, L., Gong, P., Tian, G., Nie, T."Microarray and ChIP‑seq data analysis revealed changes in p53-mediated transcriptional regulation in Nutlin-3-treated U2OS cells". Molecular Medicine Reports 12, no. 3 (2015): 4284-4290. https://doi.org/10.3892/mmr.2015.3933
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