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September-2015 Volume 12 Issue 3

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Article

Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ

  • Authors:
    • Jifeng Fan
    • Jie Li
    • Qinbo Fan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 4759-4765
    |
    Published online on: June 24, 2015
       https://doi.org/10.3892/mmr.2015.3996
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Abstract

Naringin is a dihydrotestosterone flavonoid compound that significantly inhibits bone loss, improves bone density, and enhances biomechanical anti‑compression performance. Previous studies have demonstrated that naringin improves the activity levels of osteocalcin (OC) and alkaline phosphatase (ALP) in MC3T3‑E1 osteoblast precursor cells. The present study investigated the effects of naringin on osteoblastic differentiation and inhibition of adipocyte formation in bone marrow stem cells (BMSCs). The levels of osteogenesis were modulated via upregulation of the expression levels of microRNA (miR)‑20a, and downregulation of the expression levels of peroxisome proliferator‑activated receptor γ (PPARγ). The results indicated that naringin significantly enhanced BMSC proliferation in a dose‑dependent manner. In addition, naringin significantly increased the mRNA expression levels of OC, ALP, and collagen type I. Furthermore, naringin decreased the protein expression levels of PPARγ, and increased the expression levels of miR‑20a in the BMSCs. These results suggested that miR‑20a may regulate the expression of PPARγ in BMSCs. To our knowledge, this is the first study to report naringin‑induced osteogenesis via upregulation of the expression levels of miR‑20a, and downregulation of the expression levels of PPARγ. These results indicated the important role of naringin in BMSC differentiation.
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1 

Wu G, Cui Y, Ma L, Pan X, Wang X and Zhang B: Repairing cartilage defects with bone marrow mesenchymal stem cells induced by CDMP and TGF-β1. Cell Tissue Bank. 15:51–57. 2014. View Article : Google Scholar

2 

Gong P, Wang Y, Zhang J and Wang Z: Bone marrow mesenchymal stem cells in hepatocellular carcinoma. Front Biosci (Landmark Ed). 18:811–819. 2013. View Article : Google Scholar

3 

Sotiropoulou PA, Perez SA, Salagianni M, Baxevanis CN and Papamichail M: Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells. Stem Cells. 24:462–471. 2006. View Article : Google Scholar

4 

Hu X, Wei L, Taylor TM, et al: Hypoxic preconditioning enhances bone marrow mesenchymal stem cell migration via Kv2.1 channel and FAK activation. Am J Physiol Cell Physiol. 301:C362–C372. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Pelled G, G T, Aslan H, Gazit Z and Gazit D: Mesenchymal stem cells for bone gene therapy and tissue engineering. Curr Pharm Des. 8:1917–1928. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Damoulis PD, Drakos DE, Gagari E and Kaplan DL: Osteogenic differentiation of human mesenchymal bone marrow cells in silk scaffolds is regulated by nitric oxide. Ann NY Acad Sci. 1117:367–376. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Floyd ZE and Stephens JM: Controlling a master switch of adipocyte development and insulin sensitivity: Covalent modifications of PPARγ. Biochim Biophys Acta. 1822:1090–1095. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Krishnan A, Nair SA and Pillai MR: Biology of PPAR gamma in cancer: A critical review on existing lacunae. Curr Mol Med. 7:532–540. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Takada I, Suzawa M, Matsumoto K and Kato S: Suppression of PPAR transactivation switches cell fate of bone marrow stem cells from adipocytes into osteoblasts. Ann NY Acad Sci. 1116:182–195. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Takada I and Kato S: Molecular mechanism of switching adipocyte / osteoblast differentiation through regulation of PPAR-gamma function. Clin Calcium. 18:656–661. 2008.In Japanese. PubMed/NCBI

11 

Salmanidis M, Pillman K, Goodall G and Bracken C: Direct transcriptional regulation by nuclear microRNAs. Int J Biochem Cell Biol. 54:304–311. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Yu F, Cui Y, Zhou X, Zhang X and Han J: Osteogenic differentiation of human ligament fibroblasts induced by conditioned medium of osteoclast-like cells. Biosci Trends. 5:46–51. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Zhang JF, Fu WM, He ML, Xie WD, Lv Q, Wan G, Li G, Wang H, Lu G, Hu X, et al: MiRNA-20a promotes osteogenic differentiation of human mesenchymal stem cells by co-regulating BMP signaling. RNA Biol. 8:829–838. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Li N, Jiang Y, Wooley PH, Xu Z and Yang SY: Naringin promotes osteoblast differentiation and effectively reverses ovariectomy-associated osteoporosis. J Orthop Sci. 18:478–485. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Chen LL, Lei LH, Ding PH, Tang Q and Wu YM: Osteogenic effect of Drynariae rhizoma extracts and Naringin on MC3T3-E1 cells and an induced rat alveolar bone resorption model. Arch Oral Biol. 56:1655–1662. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Surampalli G, K Nanjwade B and Patil PA: Corroboration of naringin effects on the intestinal absorption and pharmacokinetic behavior of candesartan cilexetil solid dispersions using in-situ rat models. Drug Dev Ind Pharm. June 11–2014.Epub ahead of print. PubMed/NCBI

17 

Kim HJ, Song JY, Park HJ, Park HK, Yun DH and Chung JH: Naringin protects against rotenone-induced apoptosis in human neuroblastoma SH-SY5Y Cells. Korean J Physiol Pharmacol. 13:281–285. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Heo JS, Choi SM, Kim HO, Kim EH, You J, Park T, Kim E and Kim HS: Neural transdifferentiation of human bone marrow mesenchymal stem cells on hydrophobic polymer-modified surface and therapeutic effects in an animal model of ischemic stroke. Neuroscience. 238:305–318. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Ge Z, Goh JC and Lee EH: The effects of bone marrow-derived mesenchymal stem cells and fascia wrap application to anterior cruciate ligament tissue engineering. Cell Transplant. 14:763–773. 2005. View Article : Google Scholar

20 

Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL and Chen TH: Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood. 103:1669–1675. 2004. View Article : Google Scholar

21 

Mukhopadhyay A: Perspective on liver regeneration by bone marrow-derived stem cells-a scientific realization or a paradox. Cytotherapy. 15:881–892. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Shao J, Zhang Y, Yang T, Qi J, Zhang L and Deng L: HIF-1alpha disturbs osteoblasts and osteoclasts coupling in bone remodeling by up-regulating OPG expression. In Vitro Cell Dev Biol Anim. April 10–2015.Epub ahead of print. View Article : Google Scholar

23 

Aubin JE: Advances in the osteoblast lineage. Biochem Cell Biol. 76:899–910. 1998. View Article : Google Scholar

24 

Suzuki E, Ochiai-Shino H, Aoki H, et al: Akt activation is required for TGF-beta1-induced osteoblast differentiation of MC3T3-E1 pre-osteoblasts. PLoS One. 9:e1125662014. View Article : Google Scholar

25 

Kumar VS, Rajmane AR, Adil M, Kandhare AD, Ghosh P and Bodhankar SL: Naringin ameliorates acetic acid induced colitis through modulation of endogenous oxido-nitrosative balance and DNA damage in rats. J Biomed Res. 28:132–145. 2014.PubMed/NCBI

26 

Li L, Zeng Z and Cai G: Comparison of neoeriocitrin and naringin on proliferation and osteogenic differentiation in MC3T3-E1. Phytomedicine. 18:985–989. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Wu JB, Fong YC, Tsai HY, Chen YF, Tsuzuki M and Tang CH: Naringin-induced bone morphogenetic protein-2 expression via PI3K, Akt, c-Fos/c-Jun and AP-1 pathway in osteoblasts. Eur J Pharmacol. 588:333–341. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Sun J, Wang Y, Li Y and Zhao G: Downregulation of PPARγ by miR-548d-5p suppresses the adipogenic differentiation of human bone marrow mesenchymal stem cells and enhances their osteogenic potential. J Transl Med. 12:1682014. View Article : Google Scholar

29 

Harsløf T, Tofteng CL, Husted LB, Nyegaard M, Børglum A, Carstens M, Stenkjær L, Brixen K, Eiken P, Jensen JE, et al: Polymorphisms of the peroxisome proliferator-activated receptor gamma (PPARγ) gene are associated with osteoporosis. Osteoporos Int. 22:2655–2666. 2011. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Fan J, Li J and Fan Q: Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ. Mol Med Rep 12: 4759-4765, 2015.
APA
Fan, J., Li, J., & Fan, Q. (2015). Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ. Molecular Medicine Reports, 12, 4759-4765. https://doi.org/10.3892/mmr.2015.3996
MLA
Fan, J., Li, J., Fan, Q."Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ". Molecular Medicine Reports 12.3 (2015): 4759-4765.
Chicago
Fan, J., Li, J., Fan, Q."Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ". Molecular Medicine Reports 12, no. 3 (2015): 4759-4765. https://doi.org/10.3892/mmr.2015.3996
Copy and paste a formatted citation
x
Spandidos Publications style
Fan J, Li J and Fan Q: Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ. Mol Med Rep 12: 4759-4765, 2015.
APA
Fan, J., Li, J., & Fan, Q. (2015). Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ. Molecular Medicine Reports, 12, 4759-4765. https://doi.org/10.3892/mmr.2015.3996
MLA
Fan, J., Li, J., Fan, Q."Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ". Molecular Medicine Reports 12.3 (2015): 4759-4765.
Chicago
Fan, J., Li, J., Fan, Q."Naringin promotes differentiation of bone marrow stem cells into osteoblasts by upregulating the expression levels of microRNA‑20a and downregulating the expression levels of PPARγ". Molecular Medicine Reports 12, no. 3 (2015): 4759-4765. https://doi.org/10.3892/mmr.2015.3996
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