Open Access

Ischemic preconditioning maintains the immunoreactivities of glucokinase and glucokinase regulatory protein in neurons of the gerbil hippocampal CA1 region following transient cerebral ischemia

  • Authors:
    • Young Shin Cho
    • Jun Hwi Cho
    • Bich‑Na Shin
    • Geum‑Sil Cho
    • In Hye Kim
    • Joon Ha Park
    • Ji Hyeon Ahn
    • Taek Geun Ohk
    • Byung‑Ryul Cho
    • Young‑Myeong Kim
    • Seongkweon Hong
    • Moo‑Ho Won
    • Jae‑Chul Lee
  • View Affiliations

  • Published online on: July 2, 2015     https://doi.org/10.3892/mmr.2015.4021
  • Pages: 4939-4946
  • Copyright: © Cho et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glucokinase (GK) is involved in the control of blood glucose homeostasis. In the present study, the effect of ischemic preconditioning (IPC) on the immunoreactivities of GK and its regulatory protein (GKRP) following 5 min of transient cerebral ischemia was investigated in gerbils. The gerbils were randomly assigned to four groups (sham‑operated group, ischemia‑operated group, IPC + sham‑operated group and IPC + ischemia‑operated group). IPC was induced by subjecting the gerbils to 2 min of ischemia, followed by 1 day of recovery. In the ischemia‑operated group, a significant loss of neurons was observed in the stratum pyramidale (SP) of the hippocampal CA1 region (CA1) at 5 days post‑ischemia; however, in the IPC+ischemia‑operated group, the neurons in the SP were well protected. Following immunohistochemical investigation, the immunoreactivities of GK and GKRP in the neurons of the SP were markedly decreased in the CA1, but not the CA2/3, from 2 days post‑ischemia, and were almost undetectable in the SP 5 days post‑ischemia. In the IPC + ischemia‑operated group, the immunoreactivities of GK and GKRP in the SP of the CA1 were similar to those in the sham‑group. In brief, the findings of the present study demonstrated that IPC notably maintained the immunoreactivities of GK and GKRP in the neurons of the SP of CA1 following ischemia‑reperfusion. This indicated that GK and GKRP may be necessary for neuron survival against transient cerebral ischemia.
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October-2015
Volume 12 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Cho YS, Cho JH, Shin BN, Cho GS, Kim IH, Park JH, Ahn JH, Ohk TG, Cho BR, Kim YM, Kim YM, et al: Ischemic preconditioning maintains the immunoreactivities of glucokinase and glucokinase regulatory protein in neurons of the gerbil hippocampal CA1 region following transient cerebral ischemia. Mol Med Rep 12: 4939-4946, 2015
APA
Cho, Y.S., Cho, J.H., Shin, B., Cho, G., Kim, I.H., Park, J.H. ... Lee, J. (2015). Ischemic preconditioning maintains the immunoreactivities of glucokinase and glucokinase regulatory protein in neurons of the gerbil hippocampal CA1 region following transient cerebral ischemia. Molecular Medicine Reports, 12, 4939-4946. https://doi.org/10.3892/mmr.2015.4021
MLA
Cho, Y. S., Cho, J. H., Shin, B., Cho, G., Kim, I. H., Park, J. H., Ahn, J. H., Ohk, T. G., Cho, B., Kim, Y., Hong, S., Won, M., Lee, J."Ischemic preconditioning maintains the immunoreactivities of glucokinase and glucokinase regulatory protein in neurons of the gerbil hippocampal CA1 region following transient cerebral ischemia". Molecular Medicine Reports 12.4 (2015): 4939-4946.
Chicago
Cho, Y. S., Cho, J. H., Shin, B., Cho, G., Kim, I. H., Park, J. H., Ahn, J. H., Ohk, T. G., Cho, B., Kim, Y., Hong, S., Won, M., Lee, J."Ischemic preconditioning maintains the immunoreactivities of glucokinase and glucokinase regulatory protein in neurons of the gerbil hippocampal CA1 region following transient cerebral ischemia". Molecular Medicine Reports 12, no. 4 (2015): 4939-4946. https://doi.org/10.3892/mmr.2015.4021