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Article

Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells

  • Authors:
    • Takako Hayakawa
    • Yoshitaka Yoshimura
    • Takashi Kikuiri
    • Mino Matsuno
    • Tomokazu Hasegawa
    • Kumu Fukushima
    • Kenjiro Shibata
    • Yoshiaki Deyama
    • Kuniaki Suzuki
    • Junichiro Iida
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, Hokkaido University Graduate School of Dental Medicine, Sapporo, Hokkaido 060‑8586, Japan, Department of Molecular Cell Pharmacology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Hokkaido 060‑8586, Japan, Department of Pediatric Dentistry, Hokkaido University Graduate School of Dental Medicine, Sapporo, Hokkaido 060‑8586, Japan, Department of Pediatric Dentistry, Faculty of Dentistry, Tokushima University, Tokushima 770‑8504, Japan
  • Pages: 5879-5885
    |
    Published online on: July 29, 2015
       https://doi.org/10.3892/mmr.2015.4141
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Abstract

Mechanical stress produced by orthodontic forces is a factor in the remodeling of periodontal ligaments (PDLs) and alveolar bone. It has been reported that the expression of a number of cytokines associated with osteoclastogenesis is upregulated when compressive forces act on osteoblasts and PDL cells. The present study investigated the effects of compressive forces on the formation of osteoclasts from the macrophage cell line RAW264.7. Compressive forces on osteoclasts were exerted using layers of 3, 5, 7, 9 or 14 glass cover slips on the 4th day of culture for 24 h. The number of osteoclasts was determined by counting the number of cells positive for tartrate-resistant acid phosphatase staining. Osteoclastogenesis advanced rapidly on days four and five. The number of osteoclasts with >8 nuclei peaked when the force of 7 slips was applied, which was therefore regarded as the optimal compressive force. Alterations in the expression of osteoclast-associated genes are associated with changes in the differentiation and fusion of macrophages in response to compressive forces; therefore, osteoclast-associated genes were assessed by reverse transcription quantitative polymerase chain reaction in the present study. The mRNA expression of osteoclast‑associated genes increased significantly after 3 h of optimal compression, whereas mRNA expression increased after 24 h in the control group. These findings suggested that osteoclastogenesis of macrophages was accelerated when an optimal compressive force was applied.
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Copy and paste a formatted citation
Spandidos Publications style
Hayakawa T, Yoshimura Y, Kikuiri T, Matsuno M, Hasegawa T, Fukushima K, Shibata K, Deyama Y, Suzuki K, Iida J, Iida J, et al: Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells. Mol Med Rep 12: 5879-5885, 2015.
APA
Hayakawa, T., Yoshimura, Y., Kikuiri, T., Matsuno, M., Hasegawa, T., Fukushima, K. ... Iida, J. (2015). Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells. Molecular Medicine Reports, 12, 5879-5885. https://doi.org/10.3892/mmr.2015.4141
MLA
Hayakawa, T., Yoshimura, Y., Kikuiri, T., Matsuno, M., Hasegawa, T., Fukushima, K., Shibata, K., Deyama, Y., Suzuki, K., Iida, J."Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells". Molecular Medicine Reports 12.4 (2015): 5879-5885.
Chicago
Hayakawa, T., Yoshimura, Y., Kikuiri, T., Matsuno, M., Hasegawa, T., Fukushima, K., Shibata, K., Deyama, Y., Suzuki, K., Iida, J."Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells". Molecular Medicine Reports 12, no. 4 (2015): 5879-5885. https://doi.org/10.3892/mmr.2015.4141
Copy and paste a formatted citation
x
Spandidos Publications style
Hayakawa T, Yoshimura Y, Kikuiri T, Matsuno M, Hasegawa T, Fukushima K, Shibata K, Deyama Y, Suzuki K, Iida J, Iida J, et al: Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells. Mol Med Rep 12: 5879-5885, 2015.
APA
Hayakawa, T., Yoshimura, Y., Kikuiri, T., Matsuno, M., Hasegawa, T., Fukushima, K. ... Iida, J. (2015). Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells. Molecular Medicine Reports, 12, 5879-5885. https://doi.org/10.3892/mmr.2015.4141
MLA
Hayakawa, T., Yoshimura, Y., Kikuiri, T., Matsuno, M., Hasegawa, T., Fukushima, K., Shibata, K., Deyama, Y., Suzuki, K., Iida, J."Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells". Molecular Medicine Reports 12.4 (2015): 5879-5885.
Chicago
Hayakawa, T., Yoshimura, Y., Kikuiri, T., Matsuno, M., Hasegawa, T., Fukushima, K., Shibata, K., Deyama, Y., Suzuki, K., Iida, J."Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells". Molecular Medicine Reports 12, no. 4 (2015): 5879-5885. https://doi.org/10.3892/mmr.2015.4141
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