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Article

Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method

  • Authors:
    • Chao Zhang
    • Juanli Zhu
    • Jiangcun Yang
    • Yinsheng Wan
    • Ting Ma
    • Yali Cui
  • View Affiliations / Copyright

    Affiliations: College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China, Department of Transfusion Medicine, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of Biology, Providence College, Providence, RI 02918, USA
  • Pages: 5963-5966
    |
    Published online on: July 29, 2015
       https://doi.org/10.3892/mmr.2015.4144
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Abstract

ABO genotyping is commonly used in several situations, including blood transfusion, personal identification and disease detection. The present study developed a novel method for ABO genotyping, using loop‑mediated isothermal amplification (LAMP). This method allows the simultaneous determination of six ABO genotypes under 40 min at a constant temperature of 62˚C. The genotypes of 101 blood samples were determined to be AA (n=6), AO (n=38), BB (n=12), BO (n=29), AB (n=8) and OO (n=8) by the LAMP assay. The results were compared with the phenotypes determined by serological assay and the genotypes determined by direct sequencing, and no discrepancies were observed. This novel and rapid method, with good accuracy and reasonably cost effective, provides a supplement to routine serological ABO typing and may also be useful in other point‑of‑care testing.
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1 

Landsteiner K: Zur Kenntnis der antifermentativen, lytischen und agglutinierenden Wirkungen des Blutserums und der Lymphe. Zbl Bakt. 27:357–362. 1900.

2 

Storry JR and Olsson ML: The ABO blood group system revisited: A review and update. Immunohematology. 25:48–59. 2009.PubMed/NCBI

3 

Franchini M and Liumbruno GM; ABO blood group: Old dogma, new perspectives. Clin Chem Lab Med. 51:1545–1553. 2013.PubMed/NCBI

4 

Sano R, Takahashi Y, Nakajima T, Yoshii M, Kubo R, Takahashi K, Kominato Y, Takeshita H, Yasuda T, Tsuneyama H, et al: ABO chimerism with a minor allele detected by the peptide nucleic acid-mediated polymerase chain reaction clamping method. Blood Transfus. 12:431–434. 2014.PubMed/NCBI

5 

Dentali F, Sironi AP, Ageno W, Crestani S and Franchini M: ABO blood group and vascular disease: An update. Semin Thromb Hemost. 40:49–59. 2014.PubMed/NCBI

6 

Flegel WA: ABO genotyping: The quest for clinical applications. Blood Transfus. 11:62013.

7 

Watanabe K, Ikegaya H, Hirayama K, Motani H, Iwase H, Kaneko H, Fukushima H, Akutsu T and Sakurada K: A novel method for ABO genotyping using a DNA chip. J Forensic Sci. 56(Suppl 1): S183–S187. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Watanabe G, Umetsu K, Yuasa I and Suzuki T: Amplified product length polymorphism (APLP): A novel strategy for genotyping the ABO blood group. Hum Genet. 99:34–37. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Yamamoto F, McNeill PD and Hakomori S: Genomic organization of human histo-blood group ABO genes. Glycobiology. 5:51–58. 1995. View Article : Google Scholar : PubMed/NCBI

10 

O'Keefe DS and Dobrovic A: A rapid and reliable PCR method for genotyping the ABO blood group. Hum Mutat. 2:67–70. 1993. View Article : Google Scholar : PubMed/NCBI

11 

Ross MB and de Alarcón P: Hemolytic disease of the fetus and newborn. Neoreviews. 14:e83–e88. 2013. View Article : Google Scholar

12 

Daniels G: Human blood groups: Introduction. Human Blood Groups. 3rd edition. Wiley-Blackwell; Oxford, UK: pp. 1–10. 2013, View Article : Google Scholar

13 

Xie J, Qureshi AA, Li Y and Han J: ABO blood group and incidence of skin cancer. PLoS One. 5:e119722010. View Article : Google Scholar : PubMed/NCBI

14 

Iodice S, Maisonneuve P, Botteri E, Sandri MT and Lowenfels AB: ABO blood group and cancer. Eur J Cancer. 46:3345–3350. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Franchini M, Rossi C, Mengoli C, Frattini F, Crestani S, Giacomini I, Luppi M and Bonfanti C: ABO blood group and risk of coronary artery disease. J Thromb Thrombolys. 36:286–287. 2013. View Article : Google Scholar

16 

Olsson ML, Irshaid NM, Hosseini-Maaf B, Hellberg A, Moulds MK, Sareneva H and Chester MA: Genomic analysis of clinical samples with serologic ABO blood grouping discrepancies: Identification of 15 novel A and B subgroup alleles. Blood. 98:1585–1593. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Stratton F, Renton PH and Hancock JA: Red cell agglutinability affected by disease. Nature. 181:62–63. 1958. View Article : Google Scholar : PubMed/NCBI

18 

Hoogstraten B, Rosenfield RE and Wasserman LR: Change of ABO blood type in a patient with leukemia. Transfusion. 1:32–35. 1961. View Article : Google Scholar : PubMed/NCBI

19 

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N and Hase T: Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28:e632000. View Article : Google Scholar : PubMed/NCBI

20 

Aonuma H, Badolo A, Okado K and Kanuka H: Detection of mutation by allele-specific loop-mediated isothermal amplification (AS-LAMP). Methods Mol Biol. 1039:121–127. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Mitani Y, Lezhava A, Kawai Y, Kikuchi T, Oguchi-Katayama A, Kogo Y, Itoh M, Miyagi T, Takakura H, Hoshi K, et al: Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology. Nat Methods. 4:257–262. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Parida M, Sannarangaiah S, Dash PK, Rao PV and Morita K: Loop mediated isothermal amplification (LAMP): A new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev Med Virol. 18:407–421. 2008. View Article : Google Scholar : PubMed/NCBI

23 

He J, Shi X, Yu L, Zheng X, Lan W, Jia P, Wang J and Liu H: Development and evaluation of a loop-mediated isothermal amplification assay for diagnosis of Cyprinid herpesvirus 2. J Virol Methods. 194:206–210. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang C, Zhu J, Yang J, Wan Y, Ma T and Cui Y: Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method. Mol Med Rep 12: 5963-5966, 2015.
APA
Zhang, C., Zhu, J., Yang, J., Wan, Y., Ma, T., & Cui, Y. (2015). Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method. Molecular Medicine Reports, 12, 5963-5966. https://doi.org/10.3892/mmr.2015.4144
MLA
Zhang, C., Zhu, J., Yang, J., Wan, Y., Ma, T., Cui, Y."Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method". Molecular Medicine Reports 12.4 (2015): 5963-5966.
Chicago
Zhang, C., Zhu, J., Yang, J., Wan, Y., Ma, T., Cui, Y."Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method". Molecular Medicine Reports 12, no. 4 (2015): 5963-5966. https://doi.org/10.3892/mmr.2015.4144
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Zhu J, Yang J, Wan Y, Ma T and Cui Y: Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method. Mol Med Rep 12: 5963-5966, 2015.
APA
Zhang, C., Zhu, J., Yang, J., Wan, Y., Ma, T., & Cui, Y. (2015). Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method. Molecular Medicine Reports, 12, 5963-5966. https://doi.org/10.3892/mmr.2015.4144
MLA
Zhang, C., Zhu, J., Yang, J., Wan, Y., Ma, T., Cui, Y."Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method". Molecular Medicine Reports 12.4 (2015): 5963-5966.
Chicago
Zhang, C., Zhu, J., Yang, J., Wan, Y., Ma, T., Cui, Y."Determination of ABO blood group genotypes using the real‑time loop‑mediated isothermal amplification method". Molecular Medicine Reports 12, no. 4 (2015): 5963-5966. https://doi.org/10.3892/mmr.2015.4144
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