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November-2015 Volume 12 Issue 5

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Article

MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells

  • Authors:
    • Hongyan Huang
    • Yueyong Zhu
    • Shaoyang Li
  • View Affiliations / Copyright

    Affiliations: Liver Diseases Research Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of Neurosurgery, The First People's Hospital of Taizhou, Taizhou, Zhejiang 318000, P.R. China
  • Pages: 6918-6924
    |
    Published online on: August 26, 2015
       https://doi.org/10.3892/mmr.2015.4254
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Abstract

There is currently a requirement for effective treatment strategies for human hepatocellular carcinoma (HCC), a leading cause of cancer‑associated mortality. MicroRNA-122 (miR-122), a repressor of the endogenous apoptosis regulator Bcl‑w, is frequently downregulated in HCC. Thus, it is hypothesized that the activation of miR‑122 may induce selective hepatocellular apoptosis via caspase activation in a model of HCC. In the present study, an miR‑122 mimic transfection was performed in HepG2 cells, and used to investigate the role and therapeutic potential of miR‑122 in the regulation of HCC‑derived cell lines. The apoptotic rates of HepG2 cells were significantly increased following miR‑122 mimic transfection. Reverse transcription‑polymerase chain reaction analysis revealed that Bcl‑w mRNA was significantly reduced, while the mRNA levels of caspase‑9 and caspase‑3 were markedly increased. The immunocytochemistry results supported the mRNA trends. Collectively, the present results suggest that endogenous miR‑122 contributes to HepG2 apoptosis and that transfection of mimic miR‑122 normalizes apoptotic levels in a model of HCC.
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1 

McGlynn KA and London WT: Epidemiology and natural history of hepatocellular carcinoma. Best Pract Res Clin Gastroenterol. 19:3–23. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Altekruse SF, McGlynn KA and Reichman ME: Hepatocellular carcinoma incidence, mortality, and survival trends in the United States from 1975 to 2005. J Clin Oncol. 27:1485–1491. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Azam F and Koulaouzidis A: Hepatitis B virus and hepatocarcinogenesis. Ann Hepatol. 7:125–129. 2008.PubMed/NCBI

5 

Kaihara S, Kiuchi T, Ueda M, Oike F, Fujimoto Y, Ogawa K, Kozaki K and Tanaka K: Living-donor liver transplantation for hepatocellular carcinoma. Transplantation. 75(3 Suppl): S37–S40. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Lopez PM, Villanueva A and Llovet JM: Systematic review: Evidence-based management of hepatocellular carcinoma-an updated analysis of randomized controlled trials. Aliment Pharmacol Ther. 23:1535–1547. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Nakao K, Miyaaki H and Ichikawa T: Antitumor function of microRNA-122 against hepatocellular carcinoma. J Gastroenterol. 49:589–593. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Gramantieri L, Ferracin M, Fornari F, Veronese A, Sabbioni S, Liu CG, Calin GA, Giovannini C, Ferrazzi E, Grazi GL, et al: Cyclin G1 is a target of miR-122a, a microRNA frequently down-regulated in human hepatocellular carcinoma. Cancer Res. 67:6092–6099. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Lin CJ, Gong HY, Tseng HC, Wang WL and Wu JL: miR-122 targets an anti-apoptotic gene, Bcl-w, in human hepatocellular carcinoma cell lines. Biochem Biophys Res Commun. 375:315–320. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, et al: A mammalian microRNA expression atlas based on small RNA library sequencing. Cell. 129:1401–1414. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Chang J, Nicolas E, Marks D, Sander C, Lerro A, Buendia MA, Xu C, Mason WS, Moloshok T, Bort R, et al: miR-122, a mammalian liver-specific microRNA, is processed from hcr mRNA and may downregulate the high affinity cationic amino acid transporter CAT-1. RNA Biol. 1:106–113. 2004. View Article : Google Scholar

12 

Xu T, Zhu Y, Xiong Y, Ge YY, Yun JP and Zhuang SM: MicroRNA-195 suppresses tumorigenicity and regulates G1/S transition of human hepatocellular carcinoma cells. Hepatology. 50:113–121. 2009. View Article : Google Scholar : PubMed/NCBI

13 

O'Reilly LA, Print C, Hausmann G, Moriishi K, Cory S, Huang DC and Strasser A: Tissue expression and subcellular localization of the pro-survival molecule Bcl-w. Cell Death Differ. 8:486–494. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Lee HW, Lee SS, Lee SJ and Um HD: Bcl-w is expressed in a majority of infiltrative gastric adenocarcinomas and suppresses the cancer cell death by blocking stress-activated protein kinase/c-Jun NH2-terminal kinase activation. Cancer Res. 63:1093–1100. 2003.PubMed/NCBI

15 

Bae IH, Park MJ, Yoon SH, Kang SW, Lee SS, Choi KM and Um HD: Bcl-w promotes gastric cancer cell invasion by inducing matrix metalloproteinase-2 expression via phosphoinositide 3-kinase, Akt and Sp1. Cancer Res. 66:4991–4995. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Kitamura S, Kondo S, Shinomura Y, Kanayama S, Miyazaki Y, Kiyohara T, Hiraoka S and Matsuzawa Y: Met/HGF receptor modulates bcl-w expression and inhibits apoptosis in human colorectal cancers. Br J Cancer. 83:668–673. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Gibson L, Holmgreen SP, Huang DC, Bernard O, Copeland NG, Jenkins NA, Sutherland GR, Baker E, Adams JM and Cory S: bcl-w, a novel member of the bcl-2 family, promotes cell survival. Oncogene. 13:665–675. 1996.PubMed/NCBI

18 

Ma L, Liu J, Shen J, Liu L, Wu J, Li W, Luo J, Chen Q and Qian C: Expression of miR-122 mediated by adenoviral vector induces apoptosis and cell cycle arrest of cancer cells. Cancer Biol Ther. 9:554–561. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Wu X, Wu S, Tong L, Luan T, Lin L, Lu S, Zhao W, Ma Q, Liu H and Zhong Z: miR-122 affects the viability and apoptosis of hepatocellular carcinoma cells. Scand J Gastroenterol. 44:1332–1339. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Shen L, Li J, Xu L, Ma J, Li H, Xiao X, Zhao J and Fang L: miR-497 induces apoptosis of breast cancer cells by targeting Bcl-w. Exp Ther Med. 3:475–480. 2012.PubMed/NCBI

21 

Young DD, Connelly CM, Grohmann C and Deiters A: Small molecule modifiers of microRNA miR-122 function for the treatment of hepatitis C virus infection and hepatocellular carcinoma. J Am Chem Soc. 132:7976–7981. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Martinou JC, Desagher S and Antonsson B: Cytochrome c release from mitochondria: All or nothing. Nat Cell Biol. 2:E41–E43. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Bai S, Nasser MW, Wang B, Hsu SH, Datta J, Kutay H, Yadav A, Nuovo G, Kumar P and Ghoshal K: MicroRNA-122 inhibits tumorigenic properties of hepatocellular carcinoma cells and sensitizes these cells to sorafenib. J Biol Chem. 284:32015–32027. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Huang H, Zhu Y and Li S: MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells. Mol Med Rep 12: 6918-6924, 2015.
APA
Huang, H., Zhu, Y., & Li, S. (2015). MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells. Molecular Medicine Reports, 12, 6918-6924. https://doi.org/10.3892/mmr.2015.4254
MLA
Huang, H., Zhu, Y., Li, S."MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells". Molecular Medicine Reports 12.5 (2015): 6918-6924.
Chicago
Huang, H., Zhu, Y., Li, S."MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells". Molecular Medicine Reports 12, no. 5 (2015): 6918-6924. https://doi.org/10.3892/mmr.2015.4254
Copy and paste a formatted citation
x
Spandidos Publications style
Huang H, Zhu Y and Li S: MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells. Mol Med Rep 12: 6918-6924, 2015.
APA
Huang, H., Zhu, Y., & Li, S. (2015). MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells. Molecular Medicine Reports, 12, 6918-6924. https://doi.org/10.3892/mmr.2015.4254
MLA
Huang, H., Zhu, Y., Li, S."MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells". Molecular Medicine Reports 12.5 (2015): 6918-6924.
Chicago
Huang, H., Zhu, Y., Li, S."MicroRNA-122 mimic transfection contributes to apoptosis in HepG2 cells". Molecular Medicine Reports 12, no. 5 (2015): 6918-6924. https://doi.org/10.3892/mmr.2015.4254
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