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Article

Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats

  • Authors:
    • Lixiang Wu
    • Zhenghong Zhang
    • Xiaoyan Pan
    • Zhengchao Wang
  • View Affiliations / Copyright

    Affiliations: Provincial Key Laboratory for Developmental Biology and Neurosciences, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350108, P.R. China, Department of Histology and Embryology, Jilin Medical College, Jilin, Jilin 132013, P.R. China
  • Pages: 7153-7159
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    Published online on: August 28, 2015
       https://doi.org/10.3892/mmr.2015.4268
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Abstract

Vascular endothelial growth factor (VEGF) is vital in normal and abnormal angiogenesis in the ovary, particularly during the early development of the corpus luteum in the ovary. However, the molecular regulation of the expression VEGF during luteal development in vivo remains to be fully elucidated. As the expression of VEGF is mediated by hypoxia‑inducible factor (HIF)‑1α in luteal cells cultured in vitro, determined in our previous study, the present study was performed to confirm the hypothesis that HIF‑1α is induced and then regulates the expression of VEGF and VEGF‑dependent luteal development/function in vivo. This was investigated using a pregnant rat model treated with a small‑molecule inhibitor of HIF‑1α, echinomycin (Ech). The development of the corpus luteum in the pregnant rat ovary was identified via performing assays of the serum progesterone, testosterone and estradiol concentrations by radioimmunoassay, accompanied with determination of the changes in the expression levels of HIF‑1α and VEGF by reverse transcription‑quantitative polymerase chain reaction at different days of the developmental process. On day 5, serum progesterone levels were markedly increased, whereas serum levels of testosterone and estradiol did not change significantly. On day 17, the highest level of serum progesterone was observed, however, this was not the case for testosterone and estradiol. Further analysis of the expression levels of HIF‑1α and VEGF revealed that their changes were consistent with the changes in serum levels of progesterone, which occurred in the development of the corpus luteum in the ovaries of pregnant rats. Further investigation demonstrated that Ech inhibited luteal development through inhibiting the expression of VEGF, mediated by HIF‑1α, and subsequent luteal function, which was determined by detecting changes in serum progesterone on days 8 and 14. Taken together, these results demonstrated that HIF‑1α‑mediated expression of VEGF may be one of the important mechanisms regulating ovarian luteal development in mammals in vivo, which may provide novel strategies in treatment for fertility control and for certain types of ovarian dysfunction, including polycystic ovarian syndrome, ovarian hyperstimulation syndrome and ovarian neoplasia.
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Copy and paste a formatted citation
Spandidos Publications style
Wu L, Zhang Z, Pan X and Wang Z: Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats. Mol Med Rep 12: 7153-7159, 2015.
APA
Wu, L., Zhang, Z., Pan, X., & Wang, Z. (2015). Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats. Molecular Medicine Reports, 12, 7153-7159. https://doi.org/10.3892/mmr.2015.4268
MLA
Wu, L., Zhang, Z., Pan, X., Wang, Z."Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats". Molecular Medicine Reports 12.5 (2015): 7153-7159.
Chicago
Wu, L., Zhang, Z., Pan, X., Wang, Z."Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats". Molecular Medicine Reports 12, no. 5 (2015): 7153-7159. https://doi.org/10.3892/mmr.2015.4268
Copy and paste a formatted citation
x
Spandidos Publications style
Wu L, Zhang Z, Pan X and Wang Z: Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats. Mol Med Rep 12: 7153-7159, 2015.
APA
Wu, L., Zhang, Z., Pan, X., & Wang, Z. (2015). Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats. Molecular Medicine Reports, 12, 7153-7159. https://doi.org/10.3892/mmr.2015.4268
MLA
Wu, L., Zhang, Z., Pan, X., Wang, Z."Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats". Molecular Medicine Reports 12.5 (2015): 7153-7159.
Chicago
Wu, L., Zhang, Z., Pan, X., Wang, Z."Expression and contribution of the HIF‑1α/VEGF signaling pathway to luteal development and function in pregnant rats". Molecular Medicine Reports 12, no. 5 (2015): 7153-7159. https://doi.org/10.3892/mmr.2015.4268
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