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Expression and purification of functional HMGB1 A box by fusion with SUMO

  • Authors:
    • Wen‑Song Ge
    • Jian‑Gao Fan
    • Ying‑Wei Chen
    • Lei‑Ming Xu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Shanghai Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China
    Copyright: © Ge et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6527-6532
    |
    Published online on: September 9, 2015
       https://doi.org/10.3892/mmr.2015.4308
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Abstract

High‑mobility‑group‑box chromosomal protein 1 (HMGB1) is a ubiquitous and abundant nuclear protein in eukaryotic cells. Nuclear HMGB1 serves an important role in maintaining nuclear stability under stress. However, extracellular HMGB1 exerts actions, which are distinctly different compared with these intracellular functions. HMGB1, when released extracellularly, is a potent innate signal, which initiates host defense mechanisms or tissue regeneration. HMGB1 has two DNA‑binding domains: HMG A box and B box. The HMGB1 A box exhibits an antagonistic, anti‑inflammatory effect, and is a potential therapeutic target, however, the large‑scale expression and purification of the HMGB1 A box with high efficiency remains to be reported. In the present study, a SUMO‑fusion expression system was used to express and purify high levels of functional HMGB1 A box to meet the requirements of therapeutic protein production.
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1 

Bustin M, Lehn DA and Landsman D: Structural features of the HMG chromosomal proteins and their genes. Biochim Biophys Acta. 1049:231–243. 1990. View Article : Google Scholar : PubMed/NCBI

2 

Boonyaratanakornkit V, Melvin V, Prendergast P, Altmann M, Ronfani L, Bianchi ME, Taraseviciene L, Nordeen SK, Allegretto EA and Edwards DP: High-mobility group chromatin proteins 1 and 2 functionally interact with steroid hormone receptors to enhance their DNA binding in vitro and transcriptional activity in mammalian cells. Mol Cell Biol. 18:4471–4487. 1998. View Article : Google Scholar : PubMed/NCBI

3 

Melvin VS and Edwards DP: Coregulatory proteins in steroid hormone receptor action: The role of chromatin high mobility group proteins HMG-1 and -2. Steroids. 64:576–586. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Vaccari T, Beltrame M, Ferrari S and Bianchi ME: Hmg4, a new member of the Hmg1/2 gene family. Genomics. 49:247–252. 1998. View Article : Google Scholar : PubMed/NCBI

5 

Funayama A, Shishido T, Netsu S, Narumi T, Kadowaki S, Takahashi H, Miyamoto T, Watanabe T, Woo CH, Abe J, et al: Cardiac nuclear high mobility group box 1 prevents the development of cardiac hypertrophy and heart failure. Cardiovasc Res. 99:657–664. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Tsung A, Tohme S and Billiar TR: High mobility group box-1 in sterile inflammation. J Intern Med. 276:425–443. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Dumitriu IE, Baruah P, Manfredi AA, Bianchi ME and Rovere-Querini P: HMGB1: Guiding immunity from within. Trends Immunol. 26:381–387. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Yang D, Chen Q, Yang H, Tracey KJ, Bustin M and Oppenheim JJ: High mobility group box-1 protein induces the migration and activation of human dendritic cells and acts as an alarmin. J Leukoc Biol. 81:59–66. 2007. View Article : Google Scholar

9 

Yang H, Wang H, Czura CJ and Tracey KJ: HMGB1 as a cytokine and therapeutic target. J Endotoxin Res. 8:469–472. 2002. View Article : Google Scholar

10 

Andersson U and Tracey KJ: HMGB1 in sepsis. Scand J Infect Dis. 35:577–584. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Kokkola R, Li J, Sundberg E, Aveberger AC, Palmblad K, Yang H, Tracey KJ, Andersson U and Harris HE: Successful treatment of collagen-induced arthritis in mice and rats by targeting extracellular high mobility group box chromosomal protein 1 activity. Arthritis Rheum. 48:2052–2058. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Wang H, Bloom O, Zhang M, Vishnubhakat JM, Ombrellino M, Che J, Frazier A, Yang H, Ivanova S, Borovikova L, et al: HMG-1 as a late mediator of endotoxin lethality in mice. Science. 285:248–251. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Huang Y, Yin H, Han J, Huang B, Xu J, Zheng F, Tan Z, Fang M, Rui L, Chen D, et al: Extracellular hmgb1 functions as an innate immune-mediator implicated in murine cardiac allograft acute rejection. Am J Transplant. 7:799–808. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Andrassy M, Volz HC, Igwe JC, Funke B, Eichberger SN, Kaya Z, Buss S, Autschbach F, Pleger ST, Lukic IK, et al: High-mobility group box-1 in ischemia-reperfusion injury of the heart. Circulation. 117:3216–3226. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Andersson U and Tracey KJ: HMGB1 is a therapeutic target for sterile inflammation and infection. Annu Rev Immunol. 29:139–162. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Yang H, Ochani M, Li J, Qiang X, Tanovic M, Harris HE, Susarla SM, Ulloa L, Wang H, DiRaimo R, et al: Reversing established sepsis with antagonists of endogenous high-mobility group box 1. Proc Natl Acad Sci USA. 101:296–301. 2004. View Article : Google Scholar :

17 

Butt TR, Edavettal SC, Hall JP and Mattern MR: SUMO fusion technology for difficult-to-express proteins. Protein Expr Purif. 43:1–9. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Johnson ES: Protein modification by SUMO. Annu Rev Biochem. 73:355–382. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Hochstrasser M: Evolution and function of ubiquitin-like protein-conjugation systems. Nat Cell Biol. 2:E153–E157. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Jentsch S and Pyrowolakis G: Ubiquitin and its kin: How close are the family ties? Trends Cell Biol. 10:335–342. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Müller S, Hoege C, Pyrowolakis G and Jentsch S: SUMO, ubiquitin's mysterious cousin. Nat Rev Mol Cell Biol. 2:202–210. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Malakhov MP, Mattern MR, Malakhova OA, Drinker M, Weeks SD and Butt TR: SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. J Struct Funct Genomics. 5:75–86. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, et al: Expression and purification of SARS coronavirus proteins using SUMO-fusions. Protein Expr Purif. 42:100–110. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Zhang Z, Zhang L, Zhou C and Wu H: Ketamine inhibits LPS-induced HGMB1 release in vitro and in vivo. Int Immunopharmacol. 23:14–26. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Kong X, Yuan H, Wu X, Zhang J, Zhou H, Wang M, Liu Y and Jin X: High-mobility-group box protein 1A box reduces development of sodium laurate-induced thromboangiitis obliterans in rats. J Vasc Surg. 57:194–204. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Ge WS, Fan JG, Chen YW and Xu LM: Expression and purification of functional HMGB1 A box by fusion with SUMO. Mol Med Rep 12: 6527-6532, 2015.
APA
Ge, W., Fan, J., Chen, Y., & Xu, L. (2015). Expression and purification of functional HMGB1 A box by fusion with SUMO. Molecular Medicine Reports, 12, 6527-6532. https://doi.org/10.3892/mmr.2015.4308
MLA
Ge, W., Fan, J., Chen, Y., Xu, L."Expression and purification of functional HMGB1 A box by fusion with SUMO". Molecular Medicine Reports 12.5 (2015): 6527-6532.
Chicago
Ge, W., Fan, J., Chen, Y., Xu, L."Expression and purification of functional HMGB1 A box by fusion with SUMO". Molecular Medicine Reports 12, no. 5 (2015): 6527-6532. https://doi.org/10.3892/mmr.2015.4308
Copy and paste a formatted citation
x
Spandidos Publications style
Ge WS, Fan JG, Chen YW and Xu LM: Expression and purification of functional HMGB1 A box by fusion with SUMO. Mol Med Rep 12: 6527-6532, 2015.
APA
Ge, W., Fan, J., Chen, Y., & Xu, L. (2015). Expression and purification of functional HMGB1 A box by fusion with SUMO. Molecular Medicine Reports, 12, 6527-6532. https://doi.org/10.3892/mmr.2015.4308
MLA
Ge, W., Fan, J., Chen, Y., Xu, L."Expression and purification of functional HMGB1 A box by fusion with SUMO". Molecular Medicine Reports 12.5 (2015): 6527-6532.
Chicago
Ge, W., Fan, J., Chen, Y., Xu, L."Expression and purification of functional HMGB1 A box by fusion with SUMO". Molecular Medicine Reports 12, no. 5 (2015): 6527-6532. https://doi.org/10.3892/mmr.2015.4308
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