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Article

Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro

  • Authors:
    • Xiaoling Zhu
    • Limin Cai
    • Qinggang Meng
    • Xiaoming Jin
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Hospital of Harbin, Harbin, Heilongjiang 150010, P.R. China, Department of Dermatology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150089, P.R. China, Department of Orthopaedic Surgery, The First Hospital of Harbin, Harbin, Heilongjiang 150010, P.R. China, Department of Pathology, Basic Medical Science College, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
  • Pages: 6887-6894
    |
    Published online on: September 10, 2015
       https://doi.org/10.3892/mmr.2015.4313
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Abstract

The aim of the present study was to investigate the effect of gelsolin (GSN) on the proliferation and invasion of the 786-0 clear cell renal cell carcinoma (ccRCC) cell line in vitro. A GSN overexpression lentiviral vector was constructed and transfected into 786‑0 ccRCC cells in vitro. A 3-(4,5-dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay was conducted to detect the effect of GSN on the proliferation and adhesion ability of the 786‑0 ccRCC cells, and a Transwell invasion assay was used to determine the effect of GSN on the invasion of 786‑0 ccRCC cells. In addition, the expression levels of invasion‑associated proteins, matrix metalloproteinase (MMP)2, MMP9 and E‑cadherin were analyzed by ELISA and western blotting. The MTT assay demonstrated a significantly lower optical density value for the 786‑0/GSN cells compared with that of the 786‑0/green fluorescent protein (GFP) and 786‑0 cells following 24‑ and 48‑h culture (P<0.05). The mean penetration rate of the 786‑0/GSN cells was significantly lower than that of the 786‑0/GFP and 786‑0 cells (P<0.05) according to the Transwell invasion assay. The expression levels of MMP2 and MMP9 were significantly decreased in the 786‑0/GSN cells, when compared with the 786‑0/GFP and 786‑0 cells following a 48‑h transfection, according to ELISA (P<0.001). Furthermore, in the 786‑0/GSN cells, the expression levels of MMP2 and MMP9 were markedly decreased, while the expression of E‑cadherin was markedly increased. Thus, the overexpression of GSN may inhibit the proliferation, adhesion ability and invasion of 786‑0 ccRCC cells. Additionally, GSN downregulated the expression of MMP2 and MMP9, and upregulated the expression of E‑cadherin in the 786‑0 ccRCC cells, which may have suppressed the invasion ability of the 786-0 ccRCC cells.
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1 

Bastola P, Stratton Y, Kellner E, Mikhaylova O, Yi Y, Sartor MA, Medvedovic M, Biesiada J, Meller J and Czyzyk-Krzeska MF: Folliculin contributes to VHL tumor suppressing activity in renal cancer through regulation of autophagy. PLoS One. 8:e700302013. View Article : Google Scholar : PubMed/NCBI

2 

Chow WH, Dong LM and Devesa SS: Epidemiology and risk factors for kidney cancer. Nat Rev Urol. 7:245–257. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar

4 

Kovacs G, Akhtar M, Beckwith BJ, Bugert P, Cooper CS, Delahunt B, Eble JN, Fleming S, Ljungberg B, Medeiros LJ, et al: The Heidelberg classification of renal cell tumours. J Pathol. 183:131–133. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Ramakrishnan S, Ellis L and Pili R: Histone modifications: Implications in renal cell carcinoma. Epigenomics. 5:453–462. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Kim HL, Seligson D, Liu X, Janzen N, Bui MH, Yu H, Shi T, Figlin RA, Horvath S and Belldegrun AS: Using protein expressions to predict survival in clear cell renal carcinoma. Clin Cancer Res. 10:5464–5471. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Chuang MJ, Sun KH, Tang SJ, Deng MW, Wu YH, Sung JS, Cha TL and Sun GH: Tumor-derived tumor necrosis factor-alpha promotes progression and epithelial-mesenchymal transition in renal cell carcinoma cells. Cancer Sci. 99:905–913. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Solomon JP, Page LJ, Balch WE and Kelly JW: Gelsolin amyloidosis: Genetics biochemistry pathology and possible strategies for therapeutic intervention. Crit Rev Biochem Mol Biol. 47:282–296. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Li GH, Arora PD, Chen Y, McCulloch CA and Liu P: Multifunctional roles of gelsolin in health and diseases. Med Res Rev. 32:999–1025. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Baig RM, Mahjabeen I, Sabir M, Masood N, Ali K, Malik FA and Kayani MA: Mutational spectrum of gelsolin and its down regulation is associated with breast cancer. Dis Markers. 34:71–80. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Visapää H, Bui M, Huang Y, Seligson D, Tsai H, Pantuck A, Figlin R, Rao JY, Belldegrun A, Horvath S and Palotie A: Correlation of Ki-67 and gelsolin expression to clinical outcome in renal clear cell carcinoma. Urology. 61:845–850. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Siegel R, Naishadham D and Jemal A: Cancer statistics 2012. CA Cancer J Clin. 62:10–29. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Finley DS, Pantuck AJ and Belldegrun AS: Tumor biology and prognostic factors in renal cell carcinoma. Oncologist. 16(Suppl 2): S4–S13. 2011. View Article : Google Scholar

14 

Nag S, Larsson M, Robinson RC and Burtnick LD: Gelsolin: The tail of a molecular gymnast. Cytoskeleton (Hoboken). 70:360–384. 2013. View Article : Google Scholar

15 

Arora PD, Wang Y, Bresnick A, Dawson J, Janmey PA and McCulloch CA: Collagen remodeling by phagocytosis is determined by collagen substrate topology and calcium-dependent interactions of gelsolin with nonmuscle myosin IIA in cell adhesions. Mol Biol Cell. 24:734–747. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Ke H, Zhang JY, Akiyama SK and French JE: BCL2 interaction with actin in vitro may inhibit cell motility by enhancing actin polymerization. Cell Adh Migr. 5:6–10. 2011. View Article : Google Scholar :

17 

Zhuo J, Tan EH, Yan B, Tochhawng L, Jayapal M, Koh S, Tay HK, Maciver SK, Hooi SC, Salto-Tellez M, et al: Gelsolin induces colorectal tumor cell invasion via modulation of the urokinase-type plasminogen activator cascade. PloS One. 7:e435942012. View Article : Google Scholar : PubMed/NCBI

18 

Zhu WY, Hunag YY, Liu XG, He JY, Chen DD, Zeng F, Zhou JH and Zhang YK: Prognostic evaluation of CapG gelsolin P-gp GSTP1 and Topo-II proteins in non-small cell lung cancer. Anat Rec (Hoboken). 295:208–214. 2012. View Article : Google Scholar

19 

De Corte V, Bruyneel E, Boucherie C, Mareel M, Vandekerckhove J and Gettemans J: Gelsolin-induced epithelial cell invasion is dependent on ras-rac signaling. EMBO J. 21:6781–6790. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Liao CJ, Wu TI, Huang YH, Chang TC, Wang CS, Tsai MM, Hsu CY, Tsai MH, Lai CH and Lin KH: Overexpression of gelsolin in human cervical carcinoma and its clinicopathological significance. Gynecol Oncol. 120:135–144. 2011. View Article : Google Scholar

21 

Zhan L, Zhang H, Zhang Q, Woods CG, Chen Y, Xue P, Dong J, Tokar EJ, Xu Y, Hou Y, et al: Regulatory role of KEAP1 and NRF2 in PPARγ expression and chemoresistance in human non-small-cell lung carcinoma cells. Free Radic Biol Med. 53:758–768. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Park SM, Hwang IK, Kim SY, Lee SJ, Park KS and Lee ST: Characterization of plasma gelsolin as a substrate for matrix metalloproteinases. Proteomics. 6:1192–1199. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Anbiaee R, Mojir Sheibani K, Torbati P and Jaam H: Abnormal expression of e-cadherin in gastric adenocarcinoma and its correlation with tumor histopathology and helicobacter pylori infection. Iran Red Crescent Med J. 15:218–222. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Rao J, Seligson D, Visapaa H, Horvath S, Eeva M, Michel K, Pantuck A, Belldegrun A and Palotie A: Tissue microarray analysis of cytoskeletal actin-associated biomarkers gelsolin and E-cadherin in urothelial carcinoma. Cancer. 95:1247–1257. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhu X, Cai L, Meng Q and Jin X: Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro. Mol Med Rep 12: 6887-6894, 2015.
APA
Zhu, X., Cai, L., Meng, Q., & Jin, X. (2015). Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro. Molecular Medicine Reports, 12, 6887-6894. https://doi.org/10.3892/mmr.2015.4313
MLA
Zhu, X., Cai, L., Meng, Q., Jin, X."Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro". Molecular Medicine Reports 12.5 (2015): 6887-6894.
Chicago
Zhu, X., Cai, L., Meng, Q., Jin, X."Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro". Molecular Medicine Reports 12, no. 5 (2015): 6887-6894. https://doi.org/10.3892/mmr.2015.4313
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu X, Cai L, Meng Q and Jin X: Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro. Mol Med Rep 12: 6887-6894, 2015.
APA
Zhu, X., Cai, L., Meng, Q., & Jin, X. (2015). Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro. Molecular Medicine Reports, 12, 6887-6894. https://doi.org/10.3892/mmr.2015.4313
MLA
Zhu, X., Cai, L., Meng, Q., Jin, X."Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro". Molecular Medicine Reports 12.5 (2015): 6887-6894.
Chicago
Zhu, X., Cai, L., Meng, Q., Jin, X."Gelsolin inhibits the proliferation and invasion of the 786-0 clear cell renal cell carcinoma cell line in vitro". Molecular Medicine Reports 12, no. 5 (2015): 6887-6894. https://doi.org/10.3892/mmr.2015.4313
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