Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2015 Volume 12 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2015 Volume 12 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells

  • Authors:
    • Hong‑Peng Li
    • Hai‑Bo Qiu
    • Hai‑Qin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Critical Care Medicine, Zhongda Hospital Affiliated to Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Health Service Management, School of Public Health, Fudan University, Shanghai 200433, P.R. China
  • Pages: 8289-8293
    |
    Published online on: October 23, 2015
       https://doi.org/10.3892/mmr.2015.4481
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Lipopolysaccharides (LPSs) are an important initiation factor in acute respiratory distress syndrome. The aim of the present study was to investigate the effect of LPSs on the regulation of angiotensin II (Ang II) receptors in human pulmonary microvascular endothelial cells (HPMECs). HPMECs were treated with 0, 50, 100 or 200 ng/ml LPS and incubated for 4, 8, 12 or 16 h. The expression of Ang II type 1 receptor (AT1R) and Ang II type 2 receptor (AT2R) was determined using reverse transcription‑polymerase chain reaction and western blot analysis. The affinity to Ang II was measured using a radioligand binding assay. No AT2R expression was detected with or without LPS administration in HPMECs, and LPS treatment increased the expression level of AT1R. A time‑dependent increase of AT1R transcription was observed in the 50 ng/ml group, while in the 100 and 200 ng/ml groups, the AT1R mRNA levels reached peak values at 4 h and remained unchanged. The protein level of AT1R increased significantly in a dose‑dependent manner for each incubation time period. A time‑dependent increase in the protein level was observed in the 50 and 100 ng/ml groups. As for the 200 ng/ml group, the level of AT1R reached a peak value at 8 h. Maximal binding (Bmax) significantly increased following LPS treatment and Bmax of the 50 ng/ml group exhibited a time‑dependent increase. The Bmax of the 100 and 200 ng/ml groups reached peak values at 12 and 8 h, respectively, and decreased thereafter. The dissociation constant remained unchanged following LPS treatment. Thus, treatment with LPS increased AT1R expression and its Bmax to Ang II in HPMECs, however, did not alter the affinity of AT1R to Ang II.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Li JS, Touyz RM and Schiffrin EL: Effects of AT1 and AT2 angiotensin receptor antagonists in angiotensin II-infused rats. Hypertension. 31:487–492. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Griendling KK and Ushio-Fukai M: Reactive oxygen species as mediators of angiotensin II signaling. Regul Pept. 91:21–27. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Zhang H, Schmeisser A, Garlichs CD, Plötze K, Damme U, Mügge A and Daniel WG: Angiotensin II-induced superoxide anion generation in human vascular endothelial cells: Role of membrane-bound NADH-/NADPH-oxidases. Cardiovasc Res. 44:215–222. 1999. View Article : Google Scholar

4 

Ruiz-Ortega M, Lorenzo O, Ruperez M, König S, Wittig B and Egido J: Angiotensin II activates nuclear transcription factor kappaB through AT(1) and AT(2) in vascular smooth muscle cells: Molecular mechanisms. Circ Res. 86:1266–1272. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Marshall RP, Gohlke P, Chambers RC, Howell DC, Bottoms SE, Unger T, McAnulty RJ and Laurent GJ: Angiotensin II and the fibroproliferative response to acute lung injury. Am J Physiol Lung Cell Mol Physiol. 286:L156–L164. 2004. View Article : Google Scholar

6 

Marshall RP, McAnulty RJ and Laurent GJ: Angiotensin II is mitogenic for human lung fibroblasts via activation of the type 1 receptor. Am J Respir Crit Care Med. 161:1999–2004. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Bechara RI, Pelaez A, Palacio A, Joshi PC, Hart CM, Brown LA, Raynor R and Guidot DM: Angiotensin II mediates glutathione depletion, transforming growth factor-beta1 expression and epithelial barrier dysfunction in the alcoholic rat lung. Am J Physiol Lung Cell Mol Physiol. 289:L363–L370. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B, Yang P, Sarao R, Wada T, Leong-Poi H, et al: Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature. 436:112–116. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Idell S, Kueppers F, Lippmann M, Rosen H, Niederman M and Fein A: Angiotensin converting enzyme in bronchoalveolar lavage in ARDS. Chest. 91:52–56. 1987. View Article : Google Scholar : PubMed/NCBI

10 

Brasier AR, Recinos A III and Eledrisi MS: Vascular inflammation and the renin-angiotensin system. Arterioscler Thromb Vasc Biol. 22:1257–1266. 2002. View Article : Google Scholar : PubMed/NCBI

11 

Masaki H, Kurihara T, Yamaki A, Inomata N, Nozawa Y, Mori Y, Murasawa S, Kizima K, Maruyama K, Horiuchi M, et al: Cardiac-specific overexpression of angiotensin II AT2 receptor causes attenuated response to AT1 receptor-mediated pressor and chronotropic effects. J Clin Invest. 101:527–535. 1998. View Article : Google Scholar : PubMed/NCBI

12 

Zhang H and Sun GY: Expression and regulation of AT1 receptor in rat lung microvascular endothelial cell. J Surg Res. 134:190–197. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Garcia JG, Liu F, Verin AD, Birukova A, Dechert MA, Gerthoffer WT, Bamberg JR and English D: Sphingosine 1-phosphate promotes endothelial cell barrier integrity by Edg-dependent cytoskeletal rearrangement. J Clin Invest. 108:689–701. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Birukova AA, Wu T, Tian Y, Meliton A, Sarich N, Tian X, Leff A and Birukov KG: Iloprost improves endothelial barrier function in lipopolysaccharide-induced lung injury. Eur Respir J. 41:165–176. 2013. View Article : Google Scholar :

15 

Li H, Qiu H, Xu D, Liu L, Wang L, Dig H, Yang Y and Zhou S: Establishment of radioligand binding assay for angiotensin II type 1 receptor of human pulmonary microvascular endothelial cell after lipopolysaccharide stimulation. Int J Respir. 27:1761–1764. 2007.

16 

Dincer HE, Gangopadhyay N, Wang R and Uhal BD: Norepinephrine induces alveolar epithelial apoptosis mediated by alpha-, beta- and angiotensin receptor activation. Am J Physiol Lung Cell Mol Physiol. 281:L624–L630. 2001.PubMed/NCBI

17 

Li X, Shu R, Filippatos G and Uhal BD: Apoptosis in lung injury and remodeling. J Appl Physiol (1985). 97:1535–1542. 2004. View Article : Google Scholar

18 

Chan LY, Leung JC, Tang SC, Choy CB and Lai KN: Tubular expression of angiotensin II receptors and their regulation in IgA nephropathy. J Am Soc Nephrol. 16:2306–2317. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Li JY, Avallet O, Berthelon MC, Langlois D and Saez JM: Transcriptional and translational regulation of angiotensin II type 2 receptor by angiotensin II and growth factors. Endocrinology. 140:4988–4994. 1999.PubMed/NCBI

20 

Raiden S, Nahmod K, Nahmod V, Semeniuk G, Pereira Y, Alvarez C, Giordano M and Geffner JR: Nonpeptide antagonists of AT1 receptor for angiotensin II delay the onset of acute respiratory distress syndrome. J Pharmacol Exp Ther. 303:45–51. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Sasamura H, Nakazato Y, Hayashida T, Kitamura Y, Hayashi M and Saruta T: Regulation of vascular type 1 angiotensin receptors by cytokines. Hypertension. 30:35–41. 1997. View Article : Google Scholar : PubMed/NCBI

22 

Li D, Yang B, Philips MI and Mehta JL: Proapoptotic effects of ANG II in human coronary artery endothelial cells: Role of AT1 receptor and PKC activation. Am J Physiol. 276:H786–H792. 1999.PubMed/NCBI

23 

Trowbridge IS, Collawn JF and Hopkins CR: Signal-dependent membrane protein trafficking in the endocytic pathway. Annu Rev Cell Biol. 9:129–161. 1993. View Article : Google Scholar : PubMed/NCBI

24 

Mukherjee S, Ghosh RN and Maxfield FR: Endocytosis. Physiol Rev. 77:759–803. 1997.PubMed/NCBI

25 

Marsh M and McMahon HT: The structural era of endocytosis. Science. 285:215–220. 1999. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li HP, Qiu HB and Wang HQ: Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells. Mol Med Rep 12: 8289-8293, 2015.
APA
Li, H., Qiu, H., & Wang, H. (2015). Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells. Molecular Medicine Reports, 12, 8289-8293. https://doi.org/10.3892/mmr.2015.4481
MLA
Li, H., Qiu, H., Wang, H."Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells". Molecular Medicine Reports 12.6 (2015): 8289-8293.
Chicago
Li, H., Qiu, H., Wang, H."Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells". Molecular Medicine Reports 12, no. 6 (2015): 8289-8293. https://doi.org/10.3892/mmr.2015.4481
Copy and paste a formatted citation
x
Spandidos Publications style
Li HP, Qiu HB and Wang HQ: Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells. Mol Med Rep 12: 8289-8293, 2015.
APA
Li, H., Qiu, H., & Wang, H. (2015). Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells. Molecular Medicine Reports, 12, 8289-8293. https://doi.org/10.3892/mmr.2015.4481
MLA
Li, H., Qiu, H., Wang, H."Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells". Molecular Medicine Reports 12.6 (2015): 8289-8293.
Chicago
Li, H., Qiu, H., Wang, H."Effect of lipopolysaccharide on angiotensin II type 1 receptor expression and function in human pulmonary microvascular endothelial cells". Molecular Medicine Reports 12, no. 6 (2015): 8289-8293. https://doi.org/10.3892/mmr.2015.4481
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team