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Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia

  • Authors:
    • Guomin Shen
    • Ning Ning
    • Xingsheng Zhao
    • Xi Liu
    • Guangyu Wang
    • Tianzhen Wang
    • Ran Zhao
    • Chao Yang
    • Dongmei Wang
    • Pingyuan Gong
    • Yan Shen
    • Yongjian Sun
    • Xiao Zhao
    • Yinji Jin
    • Weiwei Yang
    • Yan He
    • Lei Zhang
    • Xiaoming Jin
    • Xiaobo Li
  • View Affiliations / Copyright

    Affiliations: Department of Medical Genetics, Medical College, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China, Department of Gastrointestinal Surgery, International Hospital of Peking University, Beijing 100871, P.R. China, Department of Cardiovascular Disease, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia 010070, P.R. China, Department of Gastrointestinal Medical Oncology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, Heilongjiang 100036, P.R. China, Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 8055-8061
    |
    Published online on: October 26, 2015
       https://doi.org/10.3892/mmr.2015.4488
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Abstract

Increasing evidence has showed that hypoxia inducible factor-1 (HIF1) has an important role in hypoxia-induced lipid accumulation, a common feature of solid tumors; however, its role remains to be fully elucidated. Adipose differentiation‑related protein (ADRP), a structural protein of lipid droplets, is found to be upregulated under hypoxic conditions. In the present study, an MCF7 breast cancer cell line was used to study the role of ADRP in hypoxia‑induced lipid accumulation. It was demonstrated that hypoxia induced the gene expression of ADRP in a HIF1‑dependent manner. Increases in the mRNA and protein levels of ADRP was accompanied by increased HIF1A activity. In addition, a significant decrease in the mRNA and protein levels of ADRP were detected in presence of siRNA targeting HIF1A. Using a dual‑luciferase reporting experiment and chromatin immunoprecipitation assay, the present study demonstrated that ADRP is a direct target gene of HIF1, and identified a functional hypoxia response element localized 33 bp upstream of the transcriptional start site of the ADRP gene. Furthermore, the present study demonstrated the role of ADRP in low density liporotein (LDL) and very‑LDL uptake‑induced lipid accumulation under hypoxia. The knockdown of ADRP did not reduce HIF1‑induced lipid accumulation under hypoxia. Together, these results showed that ADRP may be not involved in HIF1-induced lipid accumulation.
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Copy and paste a formatted citation
Spandidos Publications style
Shen G, Ning N, Zhao X, Liu X, Wang G, Wang T, Zhao R, Yang C, Wang D, Gong P, Gong P, et al: Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia. Mol Med Rep 12: 8055-8061, 2015.
APA
Shen, G., Ning, N., Zhao, X., Liu, X., Wang, G., Wang, T. ... Li, X. (2015). Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia. Molecular Medicine Reports, 12, 8055-8061. https://doi.org/10.3892/mmr.2015.4488
MLA
Shen, G., Ning, N., Zhao, X., Liu, X., Wang, G., Wang, T., Zhao, R., Yang, C., Wang, D., Gong, P., Shen, Y., Sun, Y., Zhao, X., Jin, Y., Yang, W., He, Y., Zhang, L., Jin, X., Li, X."Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia". Molecular Medicine Reports 12.6 (2015): 8055-8061.
Chicago
Shen, G., Ning, N., Zhao, X., Liu, X., Wang, G., Wang, T., Zhao, R., Yang, C., Wang, D., Gong, P., Shen, Y., Sun, Y., Zhao, X., Jin, Y., Yang, W., He, Y., Zhang, L., Jin, X., Li, X."Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia". Molecular Medicine Reports 12, no. 6 (2015): 8055-8061. https://doi.org/10.3892/mmr.2015.4488
Copy and paste a formatted citation
x
Spandidos Publications style
Shen G, Ning N, Zhao X, Liu X, Wang G, Wang T, Zhao R, Yang C, Wang D, Gong P, Gong P, et al: Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia. Mol Med Rep 12: 8055-8061, 2015.
APA
Shen, G., Ning, N., Zhao, X., Liu, X., Wang, G., Wang, T. ... Li, X. (2015). Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia. Molecular Medicine Reports, 12, 8055-8061. https://doi.org/10.3892/mmr.2015.4488
MLA
Shen, G., Ning, N., Zhao, X., Liu, X., Wang, G., Wang, T., Zhao, R., Yang, C., Wang, D., Gong, P., Shen, Y., Sun, Y., Zhao, X., Jin, Y., Yang, W., He, Y., Zhang, L., Jin, X., Li, X."Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia". Molecular Medicine Reports 12.6 (2015): 8055-8061.
Chicago
Shen, G., Ning, N., Zhao, X., Liu, X., Wang, G., Wang, T., Zhao, R., Yang, C., Wang, D., Gong, P., Shen, Y., Sun, Y., Zhao, X., Jin, Y., Yang, W., He, Y., Zhang, L., Jin, X., Li, X."Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia". Molecular Medicine Reports 12, no. 6 (2015): 8055-8061. https://doi.org/10.3892/mmr.2015.4488
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