Recombinant human PDCD5 exhibits an antitumor role in hepatocellular carcinoma cells via clathrin‑dependent endocytosis

Fu,Da‑Zhi; Cheng,Ying; He,Hui; Liu,Hai‑Yang; Liu,Yong‑Feng

doi:10.3892/mmr.2015.4489

December-2015 Volume 12 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

December-2015 Volume 12 Issue 6

Full Size Image

Article

Recombinant human PDCD5 exhibits an antitumor role in hepatocellular carcinoma cells via clathrin‑dependent endocytosis

Authors:
- Da‑Zhi Fu
- Ying Cheng
- Hui He
- Hai‑Yang Liu
- Yong‑Feng Liu
View Affiliations / Copyright

Affiliations: Department of General Surgery, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
Pages: 8135-8140
|
Published online on: October 26, 2015

https://doi.org/10.3892/mmr.2015.4489
Expand metrics +

Abstract

Liver cancer is the fifth most frequently diagnosed type of cancer in men worldwide. Recombinant human programmed cell death 5 (rhPDCD5) has been shown to enter a variety of cells by clathrin-independent endocytosis. Tissue specimens from 32 hepatocellular carcinoma (HCC) patients were collected for analysis of PDCD5 expression using ELISA. It was confirmed that the pre‑operative serum levels of PDCD5 protein in patients with HCC were significantly lower than the post‑operative serum levels. Moreover, the serum PDCD5 levels were significantly correlated with portal invasion and lymph node metastasis. rhPDCD5 inhibited cell proliferation as indicated by an MTT assay, and induced apoptosis and S-phase arrest in HCC cells as demonstrated by flow cytometric analysis. Furthermore, rhPDCD5 suppressed tumor growth in established xenograft tumor models. In addition, Pitstop2 was used to block clathrin-dependent endocytosis (CDE), which confirmed that the anti‑tumor effect of rhPDCD5 in HCC cells is mediated via CDE.

View Figures

View References

1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 6:69–90. 2011. View Article : Google Scholar
2	El-Serag HB: Hepatocellular carcinoma. N Engl J Med. 365:1118–1127. 2011. View Article : Google Scholar : PubMed/NCBI
3	Liu H, Wang Y, Zhang Y, Song Q, Di C, Chen G, Tang J and Ma D: TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal. Biochem Biophys Res Commun. 254:203–210. 1999. View Article : Google Scholar : PubMed/NCBI
4	Hedenfalk I, Duggan D, Chen Y, Radmacher M, Bittner M, Simon R, Meltzer P, Gusterson B, Esteller M, Kallioniemi OP, et al: Gene-expression profiles in hereditary breast cancer. N Engl J Med. 344:539–548. 2001. View Article : Google Scholar : PubMed/NCBI
5	Yang Y, Zhao M, Li WM and Lu YY, Chen YY, Kang B and Lu YY: Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells. Apoptosis. 11:993–1001. 2006. View Article : Google Scholar : PubMed/NCBI
6	Fu DZ, Cheng Y, He H, Liu HY and Liu YF: PDCD5 expression predicts a favorable outcome in patients with hepatocellular carcinoma. Int J Oncol. 43:821–830. 2013.PubMed/NCBI
7	Wang Y, Li D, Fan H, Tian L, Zhong Y, Zhang Y, Yuan L, Jin C, Yin C and Ma D: Cellular uptake of exogenous human PDCD5 protein. J Biol Chem. 281:24803–24817. 2006. View Article : Google Scholar : PubMed/NCBI
8	Wang Y, Shi L, Song Q, Zhang M, Lou Y, Zeng Y, Ma D, Wang Y and Ke X: Recombinant human PDCD5 protein enhances chemosensitivities of hematologic malignancies. Chin Sci Bull. 54:3981–3990. 2009. View Article : Google Scholar
9	Doherty GJ and McMahon HT: Mechanisms of endocytosis. Annu Rev Biochem. 78:857–902. 2009. View Article : Google Scholar : PubMed/NCBI
10	Royle SJ: The cellular functions of clathrin. Cell Mol Life Sci. 63:1823–1832. 2006. View Article : Google Scholar : PubMed/NCBI
11	Dutta D, Williamson CD, Cole NB and Donaldson JG: Pitstop 2 is a potent inhibitor of clathrin-independent endocytosis. PLoS One. 7:e457992012. View Article : Google Scholar : PubMed/NCBI
12	Wang Y, Wang GH and Zhang QY: Determination of PDCD5 in peripheral blood serum of cancer patients. Chin J Cancer Res. 23:224–228. 2011. View Article : Google Scholar : PubMed/NCBI
13	Han XR, Sun Y and Bai XZ: The anti-tumor role and mechanism of integrated and truncated PDCD5 proteins in osteosarcoma cells. Cell Signal. 24:1713–1721. 2012. View Article : Google Scholar : PubMed/NCBI
14	Fotin A, Cheng Y, Sliz P, Grigorieff N, Harrison SC, Kirchhausen T and Walz T: Molecular model for a complete clathrin lattice from electron cryomicroscopy. Nature. 432:573–579. 2004. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Recombinant human PDCD5 exhibits an antitumor role in hepatocellular carcinoma cells via clathrin‑dependent endocytosis

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles