Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2016 Volume 13 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2016 Volume 13 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats

  • Authors:
    • Mingming Liu
    • Jiaman Dai
    • Wenyi Liu
    • Chongjian Zhao
    • Zheng Qin Yin
  • View Affiliations / Copyright

    Affiliations: Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 321-326
    |
    Published online on: November 11, 2015
       https://doi.org/10.3892/mmr.2015.4549
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Retinitis pigmentosa (RP) is a pathological condition leading to progressive visual decline resulting from continual loss of photoreceptor cells and outer nuclear layers of the retina. The aim of the present study was to explore whether melanopsin was able to restore retinal function and inhibit its degeneration by acting in a similar manner to channel rhodopsins. Royal College of Surgeons rats, which were used as an animal model of inherited retinal degeneration, were subjected to sub-retinal injection with melanopsin overexpression vector (AV‑OPN4‑GFP). Immunohistochemical and western blot analyses were used to detect the distribution and protein expression of melanopsin in the retina, revealing that melanopsin was gradually reduced with increasing age of the rats, which was due to loss of dendritic axons of intrinsically photosensitive retinal ganglion cells. Animals injected into both eyes were subjected to a behavioral open-field test, revealing that melanopsin overexpression reduced the loss of light sensitivity of the rats. In a flash electroretinography experiment, the b‑wave and response to light flash stimuli at three and five weeks following injection with AV‑OPN4‑GFP were higher compared to those in eyes injected with AV‑GFP (P<0.05). In conclusion, the present study showed that during retinal degeneration, the expression of melanopsin was significantly decreased, while vector-mediated overexpression of melanopsin delayed the loss of visual function in rats.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Sung CH, Davenport CM, Hennessey JC, Maumenee IH, Jacobson SG, Heckenlively JR, Nowakowski R, Fishman G, Gouras P and Nathans J: Rhodopsin mutations in autosomal dominant retinitis pigmentosa. Proc Natl Acad Sci USA. 88:6481–6485. 1991. View Article : Google Scholar : PubMed/NCBI

2 

Rayapudi S, Schwartz SG, Wang X and Chavis P: Vitamin A and fish oils for retinitis pigmentosa. Cochrane Database Syst Rev. 12:CD0084282013.PubMed/NCBI

3 

Zarbin MA, Arlow T and Ritch R: Regenerative nanomedicine for vision restoration. Mayo Clin Proc. 88:1480–1490. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Kamao H, Mandai M, Okamoto S, Sakai N, Suga A, Sugita S, Kiryu J and Takahashi M: Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application. Stem Cell Reports. 2:205–218. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Jiang L, Frederick JM and Baehr W: RNA interference gene therapy in dominant retinitis pigmentosa and cone-rod dystrophy mouse models caused by GCAP1 mutations. Front Mol Neurosci. 7:252014. View Article : Google Scholar : PubMed/NCBI

6 

Pan ZH, Ganjawala TH, Lu Q, Ivanova E and Zhang Z: ChR2 Mutants at L132 and T159 with improved operational light sensitivity for vision restoration. PLoS One. 9:e989242014. View Article : Google Scholar : PubMed/NCBI

7 

Nagel G, Szellas T, Huhn W, Kateriya S, Adeishvili N, Berthold P, Ollig D, Hegemann P and Bamberg E: Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc Natl Acad Sci USA. 100:13940–13945. 2013. View Article : Google Scholar

8 

Bi A, Cui J, Ma YP, Olshevskaya E, Pu M, Dizhoor AM and Pan ZH: Ectopic expression of a microbial-type rhodopsin restores visual responses in mice with photoreceptor degeneration. Neuron. 50:23–33. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Hattar S, Liao HW, Takao M, Berson DM and Yau KW: Melanopsin-containing retinal ganglion cells: Architecture, projections, and intrinsic photosensitivity. Science. 295:1065–1070. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Ramsey DJ, Ramsey KM and Vavvas DG: Genetic advances in ophthalmology: The role of melanopsin-expressing, intrinsically photosensitive retinal ganglion cells in the circadian organization of the visual system. Semin Ophthalmol. 28:406–421. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Roecklein KA, Wong PM, Miller MA, Donofry SD, Kamarck ML and Brainard GC: Melanopsin, photosensitive ganglion cells and seasonal affective disorder. Neurosci Biobehav Rev. 37:229–239. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Koizumi A, Tanaka KF and Yamanaka A: The manipulation of neural and cellular activities by ectopic expression of melanopsin. Neurosci Res. 75:3–5. 2013. View Article : Google Scholar

13 

Kyger M, Worley A and Adamus G: Autoimmune responses against photoreceptor antigens during retinal degeneration and their role in macrophage recruitment into retinas of RCS rats. J Neuroimmunol. 254:91–100. 2013. View Article : Google Scholar :

14 

McGill TJ, Prusky GT, Douglas RM, Yasumura D, Matthes MT, Lowe RJ, Duncan JL, Yang H, Ahern K, Daniello KM, et al: Discordant anatomical, electrophysiological and visual behavioral profiles of retinal degeneration in rat models of retinal degenerative disease. Invest Ophthalmol Vis Sci. 53:6232–6244. 2012. View Article : Google Scholar : PubMed/NCBI

15 

The Ministry of Science and Technology of the People's Republic of China: Guidance suggestions for the care and use of laboratory animals. Beijing, China: 2006

16 

Tao Z, Dai J, He J, Li C, Li Y and Yin ZQ: The influence of NaIO(3)-induced retinal degeneration on intra-retinal layer and the changes of expression profile/morphology of DA-ACs and mRGCS. Mol Neurobiol. 47:241–260. 2013. View Article : Google Scholar

17 

Li YC, Li CS, Chen ZS, He JR, Tao Z and Yin ZQ: A MicroRNA, mir133b, suppresses melanopsin expression mediated by failure dopaminergic amacrine cells in RCS rats. Cell Signal. 24:685–698. 2012. View Article : Google Scholar

18 

Go RE, Hwang KA, Kim SH, Lee MY, Kim CW, Jeon SY, Kim YB and Choi KC: Effects of anti-obesity drugs, phentermine and mahuang, on the behavioral patterns in Sprague-Dawley rat model. Lab Anim Res. 30:73–78. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Lin B, Koizumi A, Tanaka N, Panda S and Masland RH: Restoration of visual function in retinal degeneration mice by ectopic expression of melanopsin. Proc Natl Acad Sci USA. 105:16009–16014. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Pinilla I, Lund RD and Sauvé Y: Contribution of rod and cone pathways to the dark-adapted electroretinogram (ERG) b-wave following retinal degeneration in RCS rats. Vision Res. 44:2467–2474. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Münch M and Kawasaki A: Intrinsically photosensitive retinal ganglion cells: Classification, function and clinical implications. Curr Opin Neurol. 26:45–51. 2013. View Article : Google Scholar

22 

Pickard GE and Sollars PJ: Intrinsically photosensitive retinal ganglion cells. Rev Physiol Biochem Pharmacol. 162:59–90. 2012.

23 

Ramsey DJ, Ramsey KM and Vavvas DG: Genetic advances in ophthalmology: The role of melanopsin-expressing, intrinsically photosensitive retinal ganglion cells in the circadian organization of the visual system. Semin Ophthalmol. 28:406–421. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Semo M, Gias C, Ahmado A and Vugler A: A role for the ciliary marginal zone in the melanopsin-dependent intrinsic pupillary light reflex. Exp Eye Res. 119:8–18. 2014. View Article : Google Scholar

25 

Hu C, Hill DD and Wong KY: Intrinsic physiological properties of the five types of mouse ganglion-cell photoreceptors. JNeurophysiol. 109:1876–1889. 2013. View Article : Google Scholar

26 

Güler AD, Ecker JL, Lall GS, Haq S, Altimus CM, Liao HW, Barnard AR, Cahill H, Badea TC, Zhao H, et al: Melanopsin cells are the principal conduits for rod-cone input to non-image-forming vision. Nature. 453:102–105. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Gamlin PD, McDougal DH, Pokorny J, Smith VC, Yau KW and Dacey DM: Human and macaque pupil responses driven by melanopsin-containing retinal ganglion cells. Vision Res. 47:946–954. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Ecker JL, Dumitrescu ON, Wong KY, Alam NM, Chen SK, LeGates T, Renna JM, Prusky GT, Berson DM and Hattar S: Melanopsin-expressing retinal ganglion-cell photoreceptors: Cellular diversity and role in pattern vision. Neuron. 67:49–60. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Grünert U, Jusuf PR, Lee SC and Nguyen DT: Bipolar input to melanopsin containing ganglion cells in primate retina. Vis Neurosci. 28:39–50. 2011. View Article : Google Scholar

30 

Padilla E, Shumake J, Barrett DW, Holmes G, Sheridan EC and Gonzalez-Lima F: Novelty-evoked activity in open field predicts susceptibility to helpless behavior. Physiol Behav. 101:746–754. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu M, Dai J, Liu W, Zhao C and Yin ZQ: Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats. Mol Med Rep 13: 321-326, 2016.
APA
Liu, M., Dai, J., Liu, W., Zhao, C., & Yin, Z.Q. (2016). Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats. Molecular Medicine Reports, 13, 321-326. https://doi.org/10.3892/mmr.2015.4549
MLA
Liu, M., Dai, J., Liu, W., Zhao, C., Yin, Z. Q."Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats". Molecular Medicine Reports 13.1 (2016): 321-326.
Chicago
Liu, M., Dai, J., Liu, W., Zhao, C., Yin, Z. Q."Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats". Molecular Medicine Reports 13, no. 1 (2016): 321-326. https://doi.org/10.3892/mmr.2015.4549
Copy and paste a formatted citation
x
Spandidos Publications style
Liu M, Dai J, Liu W, Zhao C and Yin ZQ: Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats. Mol Med Rep 13: 321-326, 2016.
APA
Liu, M., Dai, J., Liu, W., Zhao, C., & Yin, Z.Q. (2016). Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats. Molecular Medicine Reports, 13, 321-326. https://doi.org/10.3892/mmr.2015.4549
MLA
Liu, M., Dai, J., Liu, W., Zhao, C., Yin, Z. Q."Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats". Molecular Medicine Reports 13.1 (2016): 321-326.
Chicago
Liu, M., Dai, J., Liu, W., Zhao, C., Yin, Z. Q."Overexpression of melanopsin in the retina restores visual function in Royal College of Surgeons rats". Molecular Medicine Reports 13, no. 1 (2016): 321-326. https://doi.org/10.3892/mmr.2015.4549
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team