Effects of total flavones from Dendranthema morifolium on vasocontraction and proliferation of vascular smooth muscle cells

Jin,Hong‑Feng; Liu,Xiao‑Wei; Tang,Yi‑Ming; Tang,Li‑Jiang; Wang,Ya‑Li; Du,Chang‑Qing

doi:10.3892/mmr.2015.4576

January-2016 Volume 13 Issue 1

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January-2016 Volume 13 Issue 1

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Article

Effects of total flavones from Dendranthema morifolium on vasocontraction and proliferation of vascular smooth muscle cells

Authors:
- Hong‑Feng Jin
- Xiao‑Wei Liu
- Yi‑Ming Tang
- Li‑Jiang Tang
- Ya‑Li Wang
- Chang‑Qing Du
View Affiliations / Copyright

Affiliations: Department of Cardiology, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, Department of Cardiology, The First Clinical Medical Institute of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
Pages: 989-993
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Published online on: November 19, 2015

https://doi.org/10.3892/mmr.2015.4576
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Abstract

Pharmacological studies have shown that the active components in Dendranthema morifolium exhibit protective effects against ischemia/reperfusion injury; however, its pharmacological action on blood vessels has not yet been investigated. The purpose of the present study was to assess the effects of the total flavones extracted from D. morifolium (Ramat.) Tzvel. cv. Hangju (FDM) on the vasocontraction and proliferation of vascular smooth muscle cells (VSMCs). The tension of rat thoracic aortic rings was measured using a mechanical force transducer attached to a recording system. FDM induced a dose‑dependent relaxation of rings with endothelium pre‑contracted by either phenylephrine (PE; 10‑6 mol/l) or a high concentration of potassium chloride (KCl; 60 mmol/l). FDM did not significantly affect the vasorelaxant effects on mechanically removed endothelium. In endothelium‑denuded aortic rings depolarized by 60 mmol/l KCl, FDM inhibited the contraction induced by Ca2+. FDM reduced the transient contraction caused by PE in a Ca2+‑free solution, but did not affect the contraction induced by phorbol ester. Furthermore, FDM inhibited the proliferation of VSMCs with or without growth stimulation by insulin. In conclusion, that the vasorelaxation induced by FDM in rat aortic rings is not dependent on the endothelium but is mediated via a reduction of the influx of extracellular Ca2+ through the voltage‑dependent and receptor‑operated channels and via the inhibition of the release of intracellular Ca2+ in VSMCs. The anti‑proliferative activity of FDM suggests that it may be beneficial in inhibiting atherosclerosis.

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1	Qin S and Wen X: Simultaneous determination of 6 active components in Chrysanthemum morifolium by HPLC. China Journal of Chinese Materia Medica. 36:1474–1477. 2011.In Chinese.
2	Yu SK, Zhang Y and Wu XQ: Nutrition component and bioactivity of Dendranthema morifolium (Ramat.) Tzvel. cv. Hangju. Chinese Food and Nutrition. 2:50–51. 2002.
3	Liang YN, Guo QS, Zhang ZY, Wang SX and Wang T: Study on dynamic accumulation of secondary metabolites content and isoenzyme activity during blossoming stages in Chrysanthemum morifolium originating from Wenxian county. Zhongguo Zhong Yao Za Zhi. 32:199–202. 2007.In Chinese. PubMed/NCBI
4	Dai M, Liu Q, Li D and Liu L: Research of material bases on antifebrile and hypetensive effects of flos chrysanthemi. Zhong Yao Cai. 24:505–506. 2001.In Chinese. PubMed/NCBI
5	Jiang H, Xia Q, Xu W and Zheng M: Chrysanthemum mori-folium attenuated the reduction of contraction of isolated rat heart and cardiomyocytes induced by ischemia/reperfusion. Pharmazie. 59:565–567. 2004.PubMed/NCBI
6	Huang H, Guo HY and Jin HF: Effects of water extract of Dendranthema morifolium on myocardial ischemia in canines. Zhongguo Zhong Yao Za Zhi. 21:307–310. 2011.
7	Guo S: Insulin signaling, resistance and metabolic syndrome: Insights from mouse models into disease mechanisms. J Endocrinol. 220:T1–T23. 2014. View Article : Google Scholar
8	Cersosimo E, Xu X and Musi N: Potential role of insulin signaling on vascular smooth muscle cell migration, proliferation, and inflammation pathways. Am J Physiol Cell Physiol. 302:C652–C657. 2012. View Article : Google Scholar
9	Chen J, Dai M and Wang Y: Paeonol inhibits proliferation of vascular smooth muscle cells stimulated by high glucose via Ras-Raf-ERK1/2 signaling pathway in coculture model. Evid Based Complement Alternat Med. 2014:4842692014. View Article : Google Scholar : PubMed/NCBI
10	Wang K, Wen L, Peng W, Li H, Zhuang J, Lu Y, Liu B, Li X, Li W and Xu Y: Vinpocetine attenuates neointimal hyper-plasia in diabetic rat carotid arteries after balloon injury. PLoS One. 9:e968942014. View Article : Google Scholar
11	Xing J, Peng K, Cao W, Lian X, Wang Q and Wang X: Effects of total flavonoids from Dracocephalum moldavica on the proliferation, migration, and adhesion molecule expression of rat vascular smooth muscle cells induced by TNF-α. Pharm Biol. 51:74–83. 2013. View Article : Google Scholar
12	Jiang D, Li D and Wu W: Inhibitory effects and mechanisms of luteolin on proliferation and migration of vascular smooth muscle cells. Nutrients. 5:1648–1659. 2013. View Article : Google Scholar : PubMed/NCBI
13	Jiang H, Xia Q, Xu W and Zeng M: Chrysanthemum mori- folium attenuated the reduction of contraction of isolated heart and cardiomyocytes induced by ischemia/reperfusion. Pharmazie. 59:565–567. 2004.PubMed/NCBI
14	Kim SH, Kang KW, Kim KW and Kim ND: Procyanidins in crataegus extract evoke endothelium-dependent vasorelaxation in rat aorta. Life Sci. 67:121–131. 2000. View Article : Google Scholar : PubMed/NCBI
15	Zhang S, Yang Y, Kone BC, Allen JC and Kahn AM: Insulin-stimulated cyclic guanosine monophosphate inhibits vascular smooth muscle cell migration by inhibiting Ca/calmodulin-dependent protein kinase II. Circulation. 107:1539–1544. 2003. View Article : Google Scholar : PubMed/NCBI
16	Ta ka hashi S, Abe T, Gotoh J and Fukuuchi Y: Substrate-dependence of reduction of MTT: A tetrazolium dye differs in cultured astroglia and neurons. Neurochem Int. 40:441–448. 2002. View Article : Google Scholar
17	Ajay M, Gilani AU and Mustafa MR: Effects of flavonoids on vascular smooth muscle of the isolated rat thoracic aorta. Life Sci. 74:603–612. 2003. View Article : Google Scholar : PubMed/NCBI
18	Jiang HD, Cai J, Xu JH, Zhou XM and Xia Q: Endothelium-dependent and direct relaxation induced by ethyl acetate extract from Flos Chrysanthemi in rat thoracic aorta. J Ethnopharmacol. 101:221–226. 2005. View Article : Google Scholar : PubMed/NCBI
19	Ji J, Benishin CG and Pang PK: Nitric oxide selectively inhibits intracellular Ca2+ release elicited by inositol trisphosphate but not caffeine in rat vascular smooth muscle. J Pharmacol Exp Ther. 285:16–21. 1998.PubMed/NCBI
20	Amberg GC and Navedo MF: Calcium dynamics in vascular smooth muscle. Microcirculation. 20:281–289. 2013. View Article : Google Scholar : PubMed/NCBI