Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
February-2016 Volume 13 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2016 Volume 13 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina

  • Authors:
    • Jian Jiang
    • Lixin Zhang
    • Lu Zhang
    • Xiaobo Xia
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 1311-1319
    |
    Published online on: December 11, 2015
       https://doi.org/10.3892/mmr.2015.4678
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Peroxisome proliferator‑activated receptor‑γ coactivator‑1α (PGC‑1α) is able to induce the expression of vascular endothelial growth factor (VEGF), promoting the formation of new blood vessels in skeletal muscle. The aim of the current study was to determine whether PGC‑1α is able to regulate angiogenesis in human retinal vascular endothelial cells (hRVECs) in vitro and in retinas in vivo. hRVECs treated with recombinant PGC‑1α were incubated for 24 h and then placed into a normoxic (20% O2) or hypoxic (1% O2) environment for a further 16 h. Following this, VEGF mRNA and protein levels were significantly increased. Cellular proliferation was enhanced by treatment with recombinant PGC‑1α in normoxic and hypoxic conditions. At 24 h following recombinant PGC‑1α treatment, hRVECs were plated into Matrigel‑coated plates and cultured under normoxic (20% O2) or hypoxic (1% O2) conditions for a further 24 h. Recombinant PGC‑1α‑treated cells were observed to form significantly greater numbers of tubes. In a C57BL/6J mouse model of ischemic retinopathy, mice received an intravitreal injection of recombinant PGC‑1α, resulting in a significant increase in VEGF mRNA and protein levels in the retina. Retinal neovascular tufts and neovascular nuclei were investigated by angiographic and cross‑sectional analysis and were observed to be significantly increased in the PGC‑1α group compared with the control group. These results indicate that PGC‑1α is able to induce angiogenesis in hRVECs and retinas, and suggests that PGC‑1α is a potential anti‑angiogenic target in retinal neovascularization.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Puigserver P, Wu Z, Park CW, Graves R, Wright M and Spiegelman BM: A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell. 92:829–839. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Larrouy D, Vidal H, Andreelli F, Laville M and Langin D: Cloning and mRNA tissue distribution of human PPARgamma coactivator-1. Int J Obes Relat Metab Disord. 23:1327–1332. 1999. View Article : Google Scholar

3 

Valle I, Alvarez-Barrientos A, Arza E, Lamas S and Monsalve M: PGC-1α regulates the mitochondrial antioxidant defense system in vascular endothelial cells. Cardiovasc Res. 66:562–573. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Borniquel S, Valle I, Cadenas S, Lamas S and Monsalve M: Nitric oxide regulates mitochondrial oxidative stress protection via the transcriptional coactivator PGC-1alpha. FASEB J. 20:1889–1891. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Barger PM, Browning AC, Garner AN and Kelly DP: p38 mitogen-activated protein kinase activates peroxisome proliferator-activated receptor α: A potential role in the cardiac metabolic stress response. J Biol Chem. 276:44495–44501. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Mascareno E, Manukyan I, Das DK and Siddiqui MA: Downregulation of cardiac lineage protein (CLP-1) expression in CLP-1 +/− mice affords cardioprotection against ischemic stress. J Cell Mol Med. 13:2744–2753. 2009. View Article : Google Scholar

7 

Chen SD, Lin TK, Yang DI, Lee SY, Shaw FZ, Liou CW and Chuang YC: Protective effects of peroxisome proliferator-activated receptors gamma coactivator-1 alpha against neuronal cell death in the hippocampal CA1 subfield after transient global ischemia. J Neurosci Res. 88:605–613. 2010. View Article : Google Scholar

8 

Gutsaeva DR, Carraway MS, Suliman HB, Demchenko IT, Shitara H, Yonekawa H and Piantadosi CA: Transient hypoxia stimulates mitochondrial biogenesis in brain subcortex by a neuronal nitric oxide synthase-dependent mechanism. J Neurosci. 28:2015–2024. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Rasbach KA and Schnellmann RG: Signaling of mitochondrial biogenesis following oxidant injury. J Biol Chem. 282:2355–2362. 2007. View Article : Google Scholar

10 

Yamaguchi T, Omori M, Tanaka N and Fukui N: Distinct and additive effects of sodium bicarbonate and continuous mild heat stress on fiber type shift via calcineurin/NFAT pathway in human skeletal myoblasts. Am J Physiol Cell Physiol. 305:C323–C333. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Yamaguchi T, Suzuki T, Arai H, Tanabe S and Atomi Y: Continuous mild heat stress induces differentiation of mammalian myoblasts, shifting fiber type from fast to slow. Am J Physiol Cell Physiol. 298:C140–C148. 2010. View Article : Google Scholar

12 

Arany Z, Foo SY, Ma Y, Ruas JL, Bommi-Reddy A, Girnun G, Cooper M, Laznik D, Chinsomboon J, Rangwala SM, et al: HIF-independent regulation of VEGF and angiogenesis by the transcriptional coactivator PGC-1alpha. Nature. 451:1008–1012. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Carmeliet P and Baes M: Metabolism and therapeutic angiogenesis. N Engl J Med. 358:2511–2512. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Gariano RF and Gardner TW: Retinal angiogenesis in development and disease. Nature. 438:960–966. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Yu DY and Cringle SJ: Oxygen distribution and consumption within the retina in vascularised and avascular retinas and in animal models of retinal disease. Prog Retin Eye Res. 20:175–208. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Bucolo C, Melilli B, Piazza C, Zurria M and Drago F: Ocular pharmacokinetics profile of different indomethacin topical formulations. J Ocul Pharmacol Ther. 27:571–576. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Smith LE, Wesolowski E, McLellan A, Kostyk SK, D'Amato R, Sullivan R and D'Amore PA: Oxygen-induced retinopathy in the mouse. Invest Ophthalmol Vis Sci. 35:101–110. 1994.PubMed/NCBI

18 

Jiang J, Xia XB, Xu HZ, Xiong Y, Song WT, Xiong SQ and Li Y: Inhibition of retinal neovascularization by gene transfer of small interfering RNA targeting HIF-1alpha and VEGF. J Cell Physiol. 218:66–74. 2009. View Article : Google Scholar

19 

Saint-Geniez M and D'Amore PA: Development and pathology of the hyaloid, choroidal and retinal vasculature. Int J Dev Biol. 48:1045–1058. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Suwa M, Nakano H and Kumagai S: Effects of chronic AICAR treatment on fiber composition, enzyme activity, UCP3, and PGC-1 in rat muscles. J Appl Physiol. 95:960–968. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Lin J, Handschin C and Spiegelman BM: Metabolic control through the PGC-1 family of transcription coactivators. Cell Metab. 1:361–370. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Wu Z, Puigserver P, Andersson U, Zhang C, Adelmant G, Mootha V, Troy A, Cinti S, Lowell B, Scarpulla RC and Spiegelman BM: Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1. Cell. 98:115–124. 1999. View Article : Google Scholar : PubMed/NCBI

23 

St-Pierre J, Lin J, Krauss S, Tarr PT, Yang R, Newgard CB and Spiegelman BM: Bioenergetic analysis of peroxisome proliferator-activated receptor gamma coactivators 1alpha and 1beta (PGC-1alpha and PGC-1beta) in muscle cells. J Biol Chem. 278:26597–26603. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Chaillou T, Koulmann N, Meunier A, Chapot R, Serrurier B, Beaudry M and Bigard X: Effect of hypoxia exposure on the recovery of skeletal muscle phenotype during regeneration. Mol Cell Biochem. 390:31–40. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Rao J, Li J, Liu Y, Lu P, Sun X, Sugumaran PK and Zhu D: The key role of PGC-1α in mitochondrial biogenesis and the proliferation of pulmonary artery vascular smooth muscle cells at an early stage of hypoxic exposure. Mol Cell Biochem. 367:9–18. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Ferrara N, Gerber HP and LeCouter J: The biology of VEGF and its receptors. Nat Med. 9:669–676. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Ferrara N: VEGF as a therapeutic target in cancer. Oncology. 69:11–16. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Arnaoutova I, George J, Kleinman HK and Benton G: The endothelial cell tube formation assay on basement membrane turns 20: State of the science and the art. Angiogenesis. 12:267–274. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Saint-Geniez M, Jiang A, Abend S, Liu L, Sweigard H, Connor KM and Arany Z: PGC-1α regulates normal and pathological angiogenesis in the retina. Am J Pathol. 182:255–265. 2013. View Article : Google Scholar :

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jiang J, Zhang L, Zhang L and Xia X: Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina. Mol Med Rep 13: 1311-1319, 2016.
APA
Jiang, J., Zhang, L., Zhang, L., & Xia, X. (2016). Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina. Molecular Medicine Reports, 13, 1311-1319. https://doi.org/10.3892/mmr.2015.4678
MLA
Jiang, J., Zhang, L., Zhang, L., Xia, X."Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina". Molecular Medicine Reports 13.2 (2016): 1311-1319.
Chicago
Jiang, J., Zhang, L., Zhang, L., Xia, X."Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina". Molecular Medicine Reports 13, no. 2 (2016): 1311-1319. https://doi.org/10.3892/mmr.2015.4678
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang J, Zhang L, Zhang L and Xia X: Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina. Mol Med Rep 13: 1311-1319, 2016.
APA
Jiang, J., Zhang, L., Zhang, L., & Xia, X. (2016). Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina. Molecular Medicine Reports, 13, 1311-1319. https://doi.org/10.3892/mmr.2015.4678
MLA
Jiang, J., Zhang, L., Zhang, L., Xia, X."Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina". Molecular Medicine Reports 13.2 (2016): 1311-1319.
Chicago
Jiang, J., Zhang, L., Zhang, L., Xia, X."Application of recombinant peroxisome proliferator‑activated receptor‑γ coactivator‑1α mediates neovascularization in the retina". Molecular Medicine Reports 13, no. 2 (2016): 1311-1319. https://doi.org/10.3892/mmr.2015.4678
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team