Open Access

Interruptin B induces brown adipocyte differentiation and glucose consumption in adipose-derived stem cells

  • Authors:
    • Sireewan Kaewsuwan
    • Anuchit Plubrukarn
    • Maleeruk Utsintong
    • Seok‑Ho Kim
    • Jin‑Hyun Jeong
    • Jin Gu Cho
    • Sang Gyu Park
    • Jong‑Hyuk Sung
  • View Affiliations

  • Published online on: January 12, 2016     https://doi.org/10.3892/mmr.2016.4758
  • Pages: 2078-2086
  • Copyright: © Kaewsuwan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Interruptin B has been isolated from Cyclosorus terminans, however, its pharamcological effect has not been fully identified. In the present study, the effects of interruptin B, from C. terminans, on brown adipocyte differentiation and glucose uptake in adipose‑derived stem cells (ASCs) were investigated. The results revealed that interruptin B dose‑dependently enhanced the adipogenic differentiation of ASCs, with an induction in the mRNA expression levels of peroxisome proliferator‑activated receptor (PPAR)‑α and PPAR‑γ. In addition, interruptin B efficiently increased the number and the membrane potential of mitochondria and upregulated the mRNA expression levels of uncoupling protein (UCP)‑1 and cyclooxygenase (COX)‑2, which are all predominantly expressed in brown adipocytes. Interruptin B increased glucose consumption in differentiated ASCs, accompanied by the upregulation in the mRNA expression levels of glucose transporter (GLUT)‑1 and GLUT‑4. The computational analysis of molecular docking, a luciferase reporter assay and surface plasmon resonance confirmed the marked binding affinity of interruptin B to PPAR‑α and PPAR‑γ (KD values of 5.32 and 0.10 µm, respectively). To the best of our knowledge, the present study is the first report to show the stimulatory effects of interruptin B on brown adipocyte differentiation and glucose uptake in ASCs, through its role as a dual PPAR‑α and PPAR‑γ ligand. Therefore, interruptin B could be further developed as a therapeutic agent for the treatment of diabetes.
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March-2016
Volume 13 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kaewsuwan S, Plubrukarn A, Utsintong M, Kim SH, Jeong JH, Cho JG, Park SG and Sung JH: Interruptin B induces brown adipocyte differentiation and glucose consumption in adipose-derived stem cells. Mol Med Rep 13: 2078-2086, 2016.
APA
Kaewsuwan, S., Plubrukarn, A., Utsintong, M., Kim, S., Jeong, J., Cho, J.G. ... Sung, J. (2016). Interruptin B induces brown adipocyte differentiation and glucose consumption in adipose-derived stem cells. Molecular Medicine Reports, 13, 2078-2086. https://doi.org/10.3892/mmr.2016.4758
MLA
Kaewsuwan, S., Plubrukarn, A., Utsintong, M., Kim, S., Jeong, J., Cho, J. G., Park, S. G., Sung, J."Interruptin B induces brown adipocyte differentiation and glucose consumption in adipose-derived stem cells". Molecular Medicine Reports 13.3 (2016): 2078-2086.
Chicago
Kaewsuwan, S., Plubrukarn, A., Utsintong, M., Kim, S., Jeong, J., Cho, J. G., Park, S. G., Sung, J."Interruptin B induces brown adipocyte differentiation and glucose consumption in adipose-derived stem cells". Molecular Medicine Reports 13, no. 3 (2016): 2078-2086. https://doi.org/10.3892/mmr.2016.4758