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Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro

  • Authors:
    • Lei Ding
    • Jingping Wu
    • Defang Li
    • Houlei Wang
    • Bin Zhu
    • Wei Lu
    • Guoxiong Xu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China, Department of Orthopedic Surgery, Second Affiliated Hospital of Anhui University of Medicine, Hefei, Anhui 230601, P.R. China, Center Laboratory, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2017-2022
    |
    Published online on: January 20, 2016
       https://doi.org/10.3892/mmr.2016.4803
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Abstract

The presence of apoptotic cells and loss of extracellular matrix (ECM) are common characteristics of degenerated cartilage endplates (CEPs). In addition, therapeutic efficacy is hampered by an incomplete understanding regarding the mechanisms underlying CEP homeostasis and degeneration. The CCN proteins have recently emerged as important regulators of cell‑ECM interactions, and have been identified as key mediators of nucleus pulposus ECM composition and tissue homeostasis. However, whether CCN3 is associated with CEP homeostasis has yet to be elucidated. The present study aimed to investigate the effects of CCN3 on the apoptosis and ECM synthesis of CEP cells cultured under serum deprivation. Rat CEP cells were confirmed to be of the chondrocytic phenotype by toluidine blue staining. The mRNA expression levels of CCN3 were markedly increased, and a dose‑dependent increase of apoptotic rate was detected under serum deprivation conditions following treatment with recombinant CCN3, whereas CCN3 did not exert a proapoptotic effect on cells cultured under normal conditions. Furthermore, CCN3‑treated cells exhibited a decrease in the expression levels of aggrecan and collagen II in both groups. These results suggested that CCN3 may act as a regulator, rather than an initiator, of serum deprivation‑induced cellular apoptosis, and that CCN3 has a catabolic effect on the mediation of ECM synthesis under both normal and serum deprivation conditions. Therefore, CCN3 may represent a novel therapeutic target for the prevention of CEP degeneration.
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1 

Hoy D, Brooks P, Woolf A, Blyth F, March L, Bain C, Baker P, Smith E and Buchbinder R: Assessing risk of bias in prevalence studies: Modification of an existing tool and evidence of interrater agreement. J Clin Epidemiol. 65:934–939. 2012. View Article : Google Scholar : PubMed/NCBI

2 

de Schepper EI, Damen J, van Meurs JB, Ginai AZ, Popham M, Hofman A, Koes BW and Bierma-Zeinstra SM: The association between lumbar disc degeneration and low back pain: The influence of age, gender, and individual radiographic features. Spine (Phila Pa 1976). 35:531–536. 2010. View Article : Google Scholar

3 

Magnier C, Boiron O, Wendling-Mansuy S, Chabrand P and Deplano V: Nutrient distribution and metabolism in the intervertebral disc in the unloaded state: A parametric study. J Biomech. 42:100–108. 2009. View Article : Google Scholar

4 

Raj PP: Intervertebral disc: Anatomy-physiology-pathophysiology-treatment. Pain Pract. 8:18–44. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Grunhagen T, Shirazi-Adl A, Fairbank JC and Urban JP: Intervertebral disk nutrition: A review of factors influencing concentrations of nutrients and metabolites. Orthop Clin North Am. 42:465–477. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Shirazi-Adl A, Taheri M and Urban JP: Analysis of cell viability in intervertebral disc: Effect of endplate permeability on cell population. J Biomech. 43:1330–1336. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Ding F, Shao ZW and Xiong LM: Cell death in intervertebral disc degeneration. Apoptosis. 18:777–785. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Kang R, Li H, Ringgaard S, Rickers K, Sun H, Chen M, Xie L and Bünger C: Interference in the endplate nutritional pathway causes intervertebral disc degeneration in an immature porcine model. Int Orthop. 38:1011–1017. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Kubota S and Takigawa M: The CCN family acting throughout the body: Recent research developments. Biomol Concepts. 4:477–494. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Chen CC and Lau LF: Functions and mechanisms of action of CCN matricellular proteins. Int J Biochem Cell Biol. 41:771–783. 2009. View Article : Google Scholar :

11 

Jun JI and Lau LF: Taking aim at the extracellular matrix: CCN proteins as emerging therapeutic targets. Nat Rev Drug Discov. 10:945–963. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Tran CM, Smith HE, Symes A, Rittié L, Perbal B, Shapiro IM and Risbud MV: Transforming growth factor β controls CCN3 expression in nucleus pulposus cells of the intervertebral disc. Arthritis Rheum. 63:3022–3031. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Bedore J, Sha W, McCann MR, Liu S, Leask A and Séguin CA: Impaired intervertebral disc development and premature disc degeneration in mice with notochord-specific deletion of CCN2. Arthritis Rheum. 65:2634–2644. 2013.PubMed/NCBI

14 

Ren Z, Hou Y, Ma S, Tao Y, Li J, Cao H and Ji L: Effects of CCN3 on fibroblast proliferation, apoptosis and extracellular matrix production. Int J Mol Med. 33:1607–1612. 2014.PubMed/NCBI

15 

Janune D, Kubota S, Nishida T, Kawaki H, Perbal B, Iida S and Takigawa M: Novel effects of CCN3 that may direct the differentiation of chondrocytes. FEBS Lett. 585:3033–3040. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Chen CC and Lau LF: Deadly liaisons: Fatal attraction between CCN matricellular proteins and the tumor necrosis factor family of cytokines. J Cell Commun Signal. 4:63–69. 2010. View Article : Google Scholar :

17 

Juric V, Chen CC and Lau LF: Fas-mediated apoptosis is regulated by the extracellular matrix protein CCN1 (CYR61) in vitro and in vivo. Mol Cell Biol. 29:3266–3279. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Chen CC, Young JL, Monzon RI, Chen N, Todorović V and Lau LF: Cytotoxicity of TNFalpha is regulated by integrin-mediated matrix signaling. EMBO J. 26:1257–1267. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Tran CM, Schoepflin ZR, Markova DZ, Kepler CK, Anderson DG, Shapiro IM and Risbud MV: CCN2 suppresses catabolic effects of interleukin-1β through α5β1 and αVβ3 integrins in nucleus pulposus cells: Implications in intervertebral disc degeneration. J Biol Chem. 289:7374–7387. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Heath E, Tahri D, Andermarcher E, Schofield P, Fleming S and Boulter CA: Abnormal skeletal and cardiac development, cardiomyopathy, muscle atrophy and cataracts in mice with a targeted disruption of the Nov (Ccn3) gene. BMC Dev Biol. 8:182008. View Article : Google Scholar : PubMed/NCBI

21 

Ivkovic S, Yoon BS, Popoff SN, Safadi FF, Libuda DE, Stephenson RC, Daluiski A and Lyons KM: Connective tissue growth factor coordinates chondrogenesis and angiogenesis during skeletal development. Development. 130:2779–2791. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Ding L, Wu JP, Xu G, Zhu B, Zeng QM, Li DF and Lu W: Lentiviral-mediated RNAi targeting caspase-3 inhibits apoptosis induced by serum deprivation in rat endplate chondrocytes in vitro. Braz J Med Biol Res. 47:445–451. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Tran CM, Markova D, Smith HE, Susarla B, Ponnappan RK, Anderson DG, Symes A, Shapiro IM and Risbud MV: Regulation of CCN2/connective tissue growth factor expression in the nucleus pulposus of the intervertebral disc: Role of Smad and activator protein 1 signaling. Arthritis Rheum. 62:1983–1992. 2010.PubMed/NCBI

24 

Tran CM, Shapiro IM and Risbud MV: Molecular regulation of CCN2 in the intervertebral disc: Lessons learned from other connective tissues. Matrix Biol. 32:298–306. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Bedore J, Leask A and Séguin CA: Targeting the extracellular matrix: Matricellular proteins regulate cell-extracellular matrix communication within distinct niches of the intervertebral disc. Matrix Biol. 37:124–130. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Kondo S, Kubota S, Mukudai Y, Moritani N, Nishida T, Matsushita H, Matsumoto S, Sugahara T and Takigawa M: Hypoxic regulation of stability of connective tissue growth factor/CCN2 mRNA by 3′-untranslated region interacting with a cellular protein in human chondrosarcoma cells. Oncogene. 25:1099–1110. 2006. View Article : Google Scholar

27 

Kivelä R, Kyröläinen H, Selänne H, Komi PV, Kainulainen H and Vihko V: A single bout of exercise with high mechanical loading induces the expression of Cyr61/CCN1 and CTGF/CCN2 in human skeletal muscle. J Appl Physiol (1985). 103:1395–1401. 2007. View Article : Google Scholar

28 

Hee HT, Chuah YJ, Tan BH, Setiobudi T and Wong HK: Vascularization and morphological changes of the endplate after axial compression and distraction of the intervertebral disc. Spine (Phila Pa 1976). 36:505–511. 2011. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Ding L, Wu J, Li D, Wang H, Zhu B, Lu W and Xu G: Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro. Mol Med Rep 13: 2017-2022, 2016.
APA
Ding, L., Wu, J., Li, D., Wang, H., Zhu, B., Lu, W., & Xu, G. (2016). Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro. Molecular Medicine Reports, 13, 2017-2022. https://doi.org/10.3892/mmr.2016.4803
MLA
Ding, L., Wu, J., Li, D., Wang, H., Zhu, B., Lu, W., Xu, G."Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro". Molecular Medicine Reports 13.3 (2016): 2017-2022.
Chicago
Ding, L., Wu, J., Li, D., Wang, H., Zhu, B., Lu, W., Xu, G."Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro". Molecular Medicine Reports 13, no. 3 (2016): 2017-2022. https://doi.org/10.3892/mmr.2016.4803
Copy and paste a formatted citation
x
Spandidos Publications style
Ding L, Wu J, Li D, Wang H, Zhu B, Lu W and Xu G: Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro. Mol Med Rep 13: 2017-2022, 2016.
APA
Ding, L., Wu, J., Li, D., Wang, H., Zhu, B., Lu, W., & Xu, G. (2016). Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro. Molecular Medicine Reports, 13, 2017-2022. https://doi.org/10.3892/mmr.2016.4803
MLA
Ding, L., Wu, J., Li, D., Wang, H., Zhu, B., Lu, W., Xu, G."Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro". Molecular Medicine Reports 13.3 (2016): 2017-2022.
Chicago
Ding, L., Wu, J., Li, D., Wang, H., Zhu, B., Lu, W., Xu, G."Effects of CCN3 on rat cartilage endplate chondrocytes cultured under serum deprivation in vitro". Molecular Medicine Reports 13, no. 3 (2016): 2017-2022. https://doi.org/10.3892/mmr.2016.4803
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