Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2016 Volume 13 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2016 Volume 13 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity

  • Authors:
    • Mengfei Wang
    • Kunting Zhu
    • Luyu Zhang
    • Lingyu Li
    • Jing Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, P.R. China, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2864-2870
    |
    Published online on: February 3, 2016
       https://doi.org/10.3892/mmr.2016.4855
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Previous studies have demonstrated that thioredoxin 1 (Trx1) exerts neuroprotective effects against cerebral ischemia/reperfusion injury caused by oxidative stress. While Trx1 is known to maintain the anti‑oxidant activity of 2‑Cys peroxiredoxins (Prdxs), the underlying mechanisms of its protective effects have remained to be elucidated, which was the aim of the present study. For this, an in vitro ischemic model of hypoxemia lasting for 4 h, followed by 24 h of reperfusion was used. Primary astrocytes from neonatal rats were pre‑treated with small interfering RNA targeting Trx1 prior to oxygen glucose deprivation/reperfusion (OGD/R). MTS and lactate dehydrogenase assays were performed to evaluate cell viability. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis were employed to assess the mRNA and protein expression levels of Prdx1‑4 and Prdx‑SO3. Furthermore, a dual luciferase reporter assay was used to assess the interaction between activator protein‑1 (AP‑1) and Trx1. The present study demonstrated that OGD/R decreased the cell viability and increased cellular damage, which was more marked following Trx1 knockdown. The expression of Prdx1‑4 and Prdx‑SO3 protein was higher in the cells subjected to OGD/R. Knockdown of Trx1 markedly decreased the levels of Prdx1‑4 but increased Prdx‑SO3 mRNA and protein levels. The results of the present study also suggested that AP‑1 directly activated the expression of Trx1. The present study demonstrated that Trx1 exerts its neuroprotective effects by preventing oxidative stress in astrocytes via maintaining Prdx expression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Liu R, Liu H, Ha Y, Tilton RG and Zhang W: Oxidative stress induces endothelial cell senescence via downregulation of Sirt6. Biomed Res Int. 9028422014.PubMed/NCBI

2 

Silva DG, Belini Junior E, de Almeida EA and Bonini-Domingos CR: Oxidative stress in sickle cell disease: An overview of erythrocyte redox metabolism and current antioxidant therapeutic strategies. Free Radic Biol Med. 65:1101–1109. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Calabrese V, Cornelius C, Mancuso C, Pennisi G, Calafato S, Bellia F, Bates TE, Giuffrida Stella AM, Schapira T, Dinkova Kostova AT and Rizzarelli E: Cellular stress response: A novel target for chemoprevention and nutritional neuroprotection in aging, neurodegenerative disorders and longevity. Neurochem Res. 33:2444–2471. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Wood ZA, Poole LB and Karplus PA: Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling. Science. 300:650–653. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Wood ZA, Schröder E, Robin Harris J and Poole LB: Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci. 28:32–40. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Soriano FX, Léveillé F, Papadia S, Higgins LG, Varley J, Baxter P, Hayes JD and Hardingham GE: Induction of sulfiredoxin expression and reduction of peroxiredoxin hyperoxidation by the neuroprotective Nrf2 activator 3H-1,2-dithiole-3-thione. J Neurochem. 107:533–543. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Das KC and Das CK: Thioredoxin, a singlet oxygen quencher and hydroxyl radical scavenger: Redox independent functions. Biochem Biophys Res Commun. 277:443–447. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Lu J and Holmgren A: Thioredoxin system in cell death progression. Antioxid Redox Signal. 17:1738–1747. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Pronk TE, van der Veen JW, Vandebriel RJ, van Loveren H, de Vink EP and Pennings JL: Comparison of the molecular topologies of stress-activated transcription factors HSF1, AP-1, NRF2 and NF-κB in their induction kinetics of HMOX1. Biosystems. 124:75–85. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Choi WJ: The Heterochromatin-1 phosphorylation contributes to TPA-Induced AP-1 expression. Biomol Ther (Seoul). 22:308–313. 2014. View Article : Google Scholar

11 

Chen B, Guan D, Cui ZJ, Wang X and Shen X: Thioredoxin 1 downregulates MCP-1 secretion and expression in human endothelial cells by suppressing nucleartranslocation of activator protein 1 and redox factor-1. Am J Physiol Cell Physiol. 298:C1170–1179. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

13 

Xing G, Dong M, Li X, Zou Y, Fan L, Wang X, Cai D, Li C, Zhou L, Liu J and Niu Y: Neuroprotective effects of puerarin against beta-amyloid-induced neurotoxicity in PC12 cells via a PI3K-dependent signaling pathway. Brain Res Bull. 85:212–218. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Guo H, Kong S, Chen W, Dai Z, Lin T, Su J, Li S, Xie Q, Su Z, Xu Y and Lai X: Apigenin mediated protection of OGD-evoked neuron-like injury in differentiated PC12 cells. Neurochem Res. 39:2197–2210. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Sengupta R and Holmgren A: Thioredoxin and thioredoxin reductase in relation to reversible S-nitrosylation. Antioxid Redox Signal. 18:259–269. 2013. View Article : Google Scholar

16 

Nickel C, Rahlfs S, Deponte M, Koncarevic S and Becker K: Thioredoxin networks in the malarial parasite Plasmodium falciparum. Antioxid Redox Signal. 8:1227–1239. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Das DK: Thioredoxin regulation of ischemic preconditioning. Antioxid Redox Signal. 26:405–412. 2004. View Article : Google Scholar

18 

Taksgi Y, Hatori I, Nozaki K, Mitsui A, Ishikawa M, Hashimoto N and Yodoi J: Excitotoxic hippocampal injury is atenuated in thioredoxin transgenic mice. J Cereb Blood Flow Metab. 20:829–833. 2000.

19 

Takagi Y, Horikawa F, Nozaki K, Sugino T, Hashimoto N and Yodoi J: Expression and distribution of redox regulatory protein, thioredoxin during transient focal brain ischemia in the rat. Neurosci Lett. 251:25–28. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Mansur K, Iwashashi Y, Kiryu-Seo S, Su Q, Namikawa K, Yodoi J and Kiyama H: Up-regulation of thioredoxin expression in motor neurons after nerve injury. Brain Res Mol Brain Res. 62:86–91. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Rhee SG, Kang SW, Jeong W, Chang TS, Yang KS and Woo HA: Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins. Curr Opin Cell Biol. 17:183–189. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Masutani H, Ueda S and Yodoi J: The thioredoxin system in retroviral infection and apoptosis. Cell Death Differ. 12(Suppl 1): 991–998. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Kondo N, Nakamura H, Masutani H and Yodoi J: Redox regulation of human thioredoxin network. Antioxid Redox Signal. 8:1881–1890. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Hwang IK, Yoo KY, Lee CH, Kim DW, Choi JH, Kwon YG, Kim YM, Choi SY and Won MH: Changes in the expression of mitochondrial peroxiredoxin and thioredoxin in neurons and glia and their protective effects in experimental cerebral ischemic damage. Free Radic Biol Med. 48:1242–1251. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Liu M, Wang Y, Zheng L, Zheng W, Dong K, Chen S, Zhang B and Li Z: Fasudil reversed MCT-induced and chronic hypoxia-induced pulmonary hypertension by attenuating oxidative stress and inhibiting the expression of Trx1 and HIF-1α. Respir Physiol Neurobiol. 201:38–46. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Bloomfield KL, Osborne SA, Kennedy DD, Clarke FM and Tonissen KF: Thioredoxin-mediated redox control of the transcription factor Sp1 and regulation of the thioredoxin gene promoter. Gene. 319:107–116. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Kim YC, Yamaguchi Y, Kondo N, Masutani H and Yodoi J: Thioredoxin-dependent redox regulation of the antioxidant responsive element (ARE) in electrophile response. Oncogene. 22:1860–1865. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Tanito M, Masutani H, Kim YC, Nishikawa M, Ohira A and Yodoi J: Sulforaphane induces thioredoxin through the antioxidant-responsive element and attenuates retinal light damage in mice. Invest Ophthalmol Vis Sci. 46:979–987. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Papadia S, Soriano FX, Léveillé F, Martel MA, Dakin KA, Hansen HH, Kaindl A, Sifringer M, Fowler J, Stefovska V, et al: Synaptic NMDA receptor activity boosts intrinsic antioxidant defenses. Nat Neurosci. 11:476–487. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Soriano FX, Baxter P, Murray LM, Sporn MB, Gillingwater TH and Hardingham GE: Transcriptional regulation of the AP-1 and Nrf2 target gene sulfiredoxin. Mol Cells. 27:279–282. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Wei Q, Jiang H, Matthews CP and Colburn NH: Sulfiredoxin is an AP-1 target gene that is required for transformation and shows elevated expression in human skin malignancies. Proc Natl Acad Sci USA. 105:19738–19743. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang M, Zhu K, Zhang L, Li L and Zhao J: Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity. Mol Med Rep 13: 2864-2870, 2016.
APA
Wang, M., Zhu, K., Zhang, L., Li, L., & Zhao, J. (2016). Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity. Molecular Medicine Reports, 13, 2864-2870. https://doi.org/10.3892/mmr.2016.4855
MLA
Wang, M., Zhu, K., Zhang, L., Li, L., Zhao, J."Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity". Molecular Medicine Reports 13.3 (2016): 2864-2870.
Chicago
Wang, M., Zhu, K., Zhang, L., Li, L., Zhao, J."Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity". Molecular Medicine Reports 13, no. 3 (2016): 2864-2870. https://doi.org/10.3892/mmr.2016.4855
Copy and paste a formatted citation
x
Spandidos Publications style
Wang M, Zhu K, Zhang L, Li L and Zhao J: Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity. Mol Med Rep 13: 2864-2870, 2016.
APA
Wang, M., Zhu, K., Zhang, L., Li, L., & Zhao, J. (2016). Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity. Molecular Medicine Reports, 13, 2864-2870. https://doi.org/10.3892/mmr.2016.4855
MLA
Wang, M., Zhu, K., Zhang, L., Li, L., Zhao, J."Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity". Molecular Medicine Reports 13.3 (2016): 2864-2870.
Chicago
Wang, M., Zhu, K., Zhang, L., Li, L., Zhao, J."Thioredoxin 1 protects astrocytes from oxidative stress by maintaining peroxiredoxin activity". Molecular Medicine Reports 13, no. 3 (2016): 2864-2870. https://doi.org/10.3892/mmr.2016.4855
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team