Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β

  • Authors:
    • Feng Xu
    • Feng Gao
    • Yadong Liu
    • Zhenyu Wang
    • Xinming Zhuang
    • Zhigang Qu
    • Hui Ma
    • Yi Liu
    • Changfeng Fu
    • Qi Zhang
    • Xiaoying Duan
  • View Affiliations

  • Published online on: February 4, 2016     https://doi.org/10.3892/mmr.2016.4861
  • Pages: 2925-2931
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Abstract

The present study aimed to explore the molecular mechanisms associated with intervertebral disc degeneration (IDD) induced by tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β. The microarray dataset no. GSE42611 was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) between four experimental nucleus pulposus samples and four control nucleus pulposus samples were analyzed. Subsequently, Gene Ontology (GO) and pathway enrichment analyses of DEGs were performed, followed by protein‑protein interaction (PPI) network construction and prediction of a regulatory network of transcription factor (TFs). Finally, the transcriptional regulatory network was integrated into the PPI network to analyze the network modules. A total of 246 upregulated and 290 downregulated DEGs were identified. The upregulated DEGs were mainly associated with GO terms linked with inflammatory response and apoptotic pathways, while the downregulated DEGs were mainly associated with GO terms linked with cell adhesion and pathways of extracellular matrix ‑ receptor interaction. In the PPI network, IL6, COL1A1, NFKB1 and HIF1A were hub genes, and in addition, NFKB1 and HIF1A were TFs. Pathways of apoptosis and extracellular matrix ‑ receptor interaction may have important roles in IDD progression. IL6, COL1A1 and the TFs NFKB1 and HIF1A may be used as biomarkers for IDD diagnosis and treatment.
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March-2016
Volume 13 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xu F, Gao F, Liu Y, Wang Z, Zhuang X, Qu Z, Ma H, Liu Y, Fu C, Zhang Q, Zhang Q, et al: Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β. Mol Med Rep 13: 2925-2931, 2016
APA
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z. ... Duan, X. (2016). Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β. Molecular Medicine Reports, 13, 2925-2931. https://doi.org/10.3892/mmr.2016.4861
MLA
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z., Ma, H., Liu, Y., Fu, C., Zhang, Q., Duan, X."Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β". Molecular Medicine Reports 13.3 (2016): 2925-2931.
Chicago
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z., Ma, H., Liu, Y., Fu, C., Zhang, Q., Duan, X."Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β". Molecular Medicine Reports 13, no. 3 (2016): 2925-2931. https://doi.org/10.3892/mmr.2016.4861