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Article

Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β

  • Authors:
    • Feng Xu
    • Feng Gao
    • Yadong Liu
    • Zhenyu Wang
    • Xinming Zhuang
    • Zhigang Qu
    • Hui Ma
    • Yi Liu
    • Changfeng Fu
    • Qi Zhang
    • Xiaoying Duan
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Orthopaedics, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China, Teaching and Research Department of Health Service Management, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China, Department of Acupuncture and Moxibustion, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China
  • Pages: 2925-2931
    |
    Published online on: February 4, 2016
       https://doi.org/10.3892/mmr.2016.4861
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Abstract

The present study aimed to explore the molecular mechanisms associated with intervertebral disc degeneration (IDD) induced by tumor necrosis factor (TNF)‑α and interleukin (IL)‑1β. The microarray dataset no. GSE42611 was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) between four experimental nucleus pulposus samples and four control nucleus pulposus samples were analyzed. Subsequently, Gene Ontology (GO) and pathway enrichment analyses of DEGs were performed, followed by protein‑protein interaction (PPI) network construction and prediction of a regulatory network of transcription factor (TFs). Finally, the transcriptional regulatory network was integrated into the PPI network to analyze the network modules. A total of 246 upregulated and 290 downregulated DEGs were identified. The upregulated DEGs were mainly associated with GO terms linked with inflammatory response and apoptotic pathways, while the downregulated DEGs were mainly associated with GO terms linked with cell adhesion and pathways of extracellular matrix ‑ receptor interaction. In the PPI network, IL6, COL1A1, NFKB1 and HIF1A were hub genes, and in addition, NFKB1 and HIF1A were TFs. Pathways of apoptosis and extracellular matrix ‑ receptor interaction may have important roles in IDD progression. IL6, COL1A1 and the TFs NFKB1 and HIF1A may be used as biomarkers for IDD diagnosis and treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Xu F, Gao F, Liu Y, Wang Z, Zhuang X, Qu Z, Ma H, Liu Y, Fu C, Zhang Q, Zhang Q, et al: Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β. Mol Med Rep 13: 2925-2931, 2016.
APA
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z. ... Duan, X. (2016). Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β. Molecular Medicine Reports, 13, 2925-2931. https://doi.org/10.3892/mmr.2016.4861
MLA
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z., Ma, H., Liu, Y., Fu, C., Zhang, Q., Duan, X."Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β". Molecular Medicine Reports 13.3 (2016): 2925-2931.
Chicago
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z., Ma, H., Liu, Y., Fu, C., Zhang, Q., Duan, X."Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β". Molecular Medicine Reports 13, no. 3 (2016): 2925-2931. https://doi.org/10.3892/mmr.2016.4861
Copy and paste a formatted citation
x
Spandidos Publications style
Xu F, Gao F, Liu Y, Wang Z, Zhuang X, Qu Z, Ma H, Liu Y, Fu C, Zhang Q, Zhang Q, et al: Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β. Mol Med Rep 13: 2925-2931, 2016.
APA
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z. ... Duan, X. (2016). Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β. Molecular Medicine Reports, 13, 2925-2931. https://doi.org/10.3892/mmr.2016.4861
MLA
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z., Ma, H., Liu, Y., Fu, C., Zhang, Q., Duan, X."Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β". Molecular Medicine Reports 13.3 (2016): 2925-2931.
Chicago
Xu, F., Gao, F., Liu, Y., Wang, Z., Zhuang, X., Qu, Z., Ma, H., Liu, Y., Fu, C., Zhang, Q., Duan, X."Bioinformatics analysis of molecular mechanisms involved in intervertebral disc degeneration induced by TNF‑α and IL‑1β". Molecular Medicine Reports 13, no. 3 (2016): 2925-2931. https://doi.org/10.3892/mmr.2016.4861
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