Nuclear distribution of eIF3g and its interacting nuclear proteins in breast cancer cells

Zheng,Qiaoli; Liu,Hao; Ye,Jingjia; Zhang,Hui; Jia,Zhenyu; Cao,Jiang

doi:10.3892/mmr.2016.4935

Nuclear distribution of eIF3g and its interacting nuclear proteins in breast cancer cells

Authors:
- Qiaoli Zheng
- Hao Liu
- Jingjia Ye
- Hui Zhang
- Zhenyu Jia
- Jiang Cao
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Affiliations: Clinical Research Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China, Institute of Occupational Diseases, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310007, P.R. China, Department of Clinical Laboratory, Hangzhou First People's Hospital, Hangzhou, Zhejiang 310006, P.R. China
Published online on: February 23, 2016 https://doi.org/10.3892/mmr.2016.4935
Pages: 2973-2980
Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Eukaryotic translation initiation factor 3 subunit g (eIF3g) is a core subunit of the eukaryotic translation initiation factor 3 complex, and is important in the initiation of translation. It is also involved in caspase-mediated apoptosis, and is upregulated in multidrug-resistant cancer cells. In the present study, the nuclear distribution of eIF3g was determined by performing co-immunoprecipitation of proteins that potentially interact with eIF3g in the nucleus. Mass spectrometry characterization showed that three proteins, heterogeneous nuclear ribonucleoprotein U/scaffold attachment factor A, HSZFP36/zinc finger protein 823 and β‑actin, were among the candidate eIF3g‑interacting proteins in the nucleus. The protein‑protein interaction was further confirmed by cross‑linking and a glutathione S‑transferase pull‑down assay, followed by western blotting. The co‑localization of these proteins was determined by confocal microscopy. These findings provide novel insight into the possible functions of eIF3g in the nucleus and serves as an important first step for further investigation of the roles of eIF3g in cancer development.

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