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Article

Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF

  • Authors:
    • Lin Quan
    • Hua He
  • View Affiliations / Copyright

    Affiliations: Department of Visual Science and Optometry, The People's Hospital of Guangxi, Nanning, Guangxi 530021, P.R. China, Ocular Surface Center, Miami, FL 33173, USA
  • Pages: 3319-3325
    |
    Published online on: February 23, 2016
       https://doi.org/10.3892/mmr.2016.4937
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Abstract

The aim of the current study was to investigate whether olopatadine and naphazoline hydrochloride reduce allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF. An allergic conjunctivitis mouse model was established using histamine or an antigen (ovalbumin), following which mice were treated with 1% olopatadine solution and/or 0.2 mg/ml of naphazoline hydrochloride. Histamine or antigen‑induced conjunctival vascular hyperpermeability was examined and the levels of inflammatory factors, cytokines, IgE, GMCSF and NGF were analyzed using ELISA in antigen‑induced conjunctival vascular hyperpermeability mice. In addition, VEGF protein expression was measured using western blotting in antigen‑induced mice. The results indicated that olopatadine and naphazoline hydrochloride significantly suppressed conjunctival dye leakage in mice with histamine or antigen‑induced conjunctival vascular hyperpermeability. In addition, treatment with olopatadine and naphazoline hydrochloride was able to reduce the levels of inflammatory factors (TNF‑α, IL‑1β and IL‑6), cytokines (IFN‑γ and IL‑4), IgE, GMCSF, and NGF in antigen‑induced conjunctival vascular hyperpermeability mice. The protein expression levels of VEGF in antigen‑induced conjunctival vascular hyperpermeability mice were reduced following treatment with olopatadine and naphazoline hydrochloride. These results suggest that treatment with olopatadine and naphazoline hydrochloride reduces conjunctivitis in mice via effects on inflammation, NGF and VEGF.
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Copy and paste a formatted citation
Spandidos Publications style
Quan L and He H: Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF. Mol Med Rep 13: 3319-3325, 2016.
APA
Quan, L., & He, H. (2016). Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF. Molecular Medicine Reports, 13, 3319-3325. https://doi.org/10.3892/mmr.2016.4937
MLA
Quan, L., He, H."Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF". Molecular Medicine Reports 13.4 (2016): 3319-3325.
Chicago
Quan, L., He, H."Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF". Molecular Medicine Reports 13, no. 4 (2016): 3319-3325. https://doi.org/10.3892/mmr.2016.4937
Copy and paste a formatted citation
x
Spandidos Publications style
Quan L and He H: Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF. Mol Med Rep 13: 3319-3325, 2016.
APA
Quan, L., & He, H. (2016). Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF. Molecular Medicine Reports, 13, 3319-3325. https://doi.org/10.3892/mmr.2016.4937
MLA
Quan, L., He, H."Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF". Molecular Medicine Reports 13.4 (2016): 3319-3325.
Chicago
Quan, L., He, H."Treatment with olopatadine and naphazoline hydrochloride reduces allergic conjunctivitis in mice through alterations in inflammation, NGF and VEGF". Molecular Medicine Reports 13, no. 4 (2016): 3319-3325. https://doi.org/10.3892/mmr.2016.4937
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