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Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas

  • Authors:
    • Ruigang Zhou
    • Yigang Man
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Neurology and Rehabilitation, Shandong Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3491-3497
    |
    Published online on: February 29, 2016
       https://doi.org/10.3892/mmr.2016.4943
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Abstract

The present study performed an integral analysis of the gene expression and DNA methylation profile of pilocytic astrocytomas (PAs). Weighted gene co-expression network analysis (WGCNA) was also performed to examine and identify the genes correlated to PAs, to identify candidate therapeutic targets for the treatment of PAs. The DNA methylation profile and gene expression profile were downloaded from the Gene Expression Omnibus database. Following screening of the differentially expressed genes (DEGs) and differentially methylated regions (DMRs), respectively, integrated analysis of the DEGs and DMRs was performed to detect their correlation. Subsequently, the WGCNA algorithm was applied to identify the significant modules and construct the co‑expression network associated with PAs. Furthermore, Gene Ontology enrichment analysis of the associated genes was performed using the Database for Annotation, Visualization and Integrated Discovery. A total number of 2,259 DEGs and 235 DMRs were screened out. Integrated analysis revealed that 30 DEGs were DMRs with prominent negative correlation (cor=‑0.82; P=0.02). Based on the DEGs, the gene co‑expression network was constructed, and nine network modules associated with PAs were identified. The functional analysis results showed that genes relevant to PAs were closely associated with cell differentiation modulation. The screened PA-associated genes were significantly different at the expression and methylation levels. These genes may be used as reliable candidate target genes for the treatment of PAs.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou R and Man Y: Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas. Mol Med Rep 13: 3491-3497, 2016.
APA
Zhou, R., & Man, Y. (2016). Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas. Molecular Medicine Reports, 13, 3491-3497. https://doi.org/10.3892/mmr.2016.4943
MLA
Zhou, R., Man, Y."Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas". Molecular Medicine Reports 13.4 (2016): 3491-3497.
Chicago
Zhou, R., Man, Y."Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas". Molecular Medicine Reports 13, no. 4 (2016): 3491-3497. https://doi.org/10.3892/mmr.2016.4943
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou R and Man Y: Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas. Mol Med Rep 13: 3491-3497, 2016.
APA
Zhou, R., & Man, Y. (2016). Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas. Molecular Medicine Reports, 13, 3491-3497. https://doi.org/10.3892/mmr.2016.4943
MLA
Zhou, R., Man, Y."Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas". Molecular Medicine Reports 13.4 (2016): 3491-3497.
Chicago
Zhou, R., Man, Y."Integrated analysis of DNA methylation profiles and gene expression profiles to identify genes associated with pilocytic astrocytomas". Molecular Medicine Reports 13, no. 4 (2016): 3491-3497. https://doi.org/10.3892/mmr.2016.4943
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