Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
April-2016 Volume 13 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2016 Volume 13 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Impaired function of the intestinal barrier in a novel sub-health rat model

  • Authors:
    • Sisi Feng
    • Weidong Liu
    • Shengnan Zuo
    • Tingyan Xie
    • Hui Deng
    • Qiuhuan Zhang
    • Baiyun Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Clinical Laboratory, Hunan Guangxiu Hospital, Changsha, Hunan 410000, P.R. China, Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3459-3465
    |
    Published online on: March 4, 2016
       https://doi.org/10.3892/mmr.2016.4978
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Sub-health is a state featuring a deterioration in physiological function between health and illness, and the sub-health condition has surfaced as life-threatening in humans. The aim of the present study was to establish a sub-health model in rats, and investigate the function of the intestinal barrier in the sub-health rats and rats following intervention. To establish a sub‑health model, the rats were subjected to a high‑fat and sugar diet, motion restriction and chronic stress. Their serum glucose and triglyceride levels, immune function and adaptability were then measured. The levels of diamine oxidase and D‑lactic acid in the plasma were analyzed as markers of the intestinal permeability. The protein and mRNA expression levels of anti‑apoptotic YWHAZ in the colonic tissue was detected using immunohistochemical and reverse transcription‑quantitative polymerase chain reaction analyses In the present study, the sub‑health rat model was successfully established, and sub‑health factors increased the intestinal permeability and reduced the expression of YWHAZ. Providing sub‑health rats with normal living conditions did not improve the function of the intestinal barrier. In conclusion, the results of the present study demonstrated that intestinal disorders in the sub‑health rat model may result from the damage caused by reduce intestinal barrier function as well as the decreased expression levels of YWHAZ. Additionally, rats in the sub‑health condition did not recover following subsequent exposure to normal living conditions, suggesting that certain exercises or medical intervention may be necessary to improve sub-health symptoms.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

World Health Organization: Preamble to the constitution of the World Health Organization as adopted by the International Health Conference; New York. 19–22 June, 1946; signed on 22 July 1946 by the representatives of 61 states. Official Record of the World Health Organization; 2. pp. 1001946

2 

Yang ZM, Yang XB and Huang L: A literature review on the conceptual framework of sub-health. Zhongguo Zhong Xi Yi Jie He Za Zhi. 30:757–763. 2010.In Chinese. PubMed/NCBI

3 

Zhang YJ, Wang TF, Xue XL, Wang JJ, Li GR and Han P: Characteristics of traditional Chinese medicine syndromes and their element distributions in sub-health status: A modern literature review. Zhong Xi Yi Jie He Xue Bao. 6:1290–1293. 2008.In Chinese. View Article : Google Scholar : PubMed/NCBI

4 

Zhou Y, You LM, Zhang JB and Guan NH: Causes and prevention of sub-health. Chinese Journal of Health Education. 18:714–715. 2002.In Chinese.

5 

Xing L: Risk factors and nursing intervention of sub-health. Medical Information. 28:2062015.In Chinese.

6 

Vazquez-Roque MI, Camilleri M, Smyrk T, Murray JA, O'Neill J, Carlson P, Lamsam J, Eckert D, Janzow D, Burton D, et al: Association of HLA-DQ gene with bowel transit, barrier function and inflammation in irritable bowel syndrome with diarrhea. Am J Physiol Gastrointest Liver Physiol. 303:G1262–G1269. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Zuhl M, Schneider S, Lanphere K, Conn C, Dokladny K and Moseley P: Exercise regulation of intestinal tight junction proteins. Br J Sports Med. 48:980–986. 2014. View Article : Google Scholar

8 

Deng H, Zhang YL, Zhong BY, Liu WD, Yan Q, Feng SS and Xiao ZQ: Clinical application and identification of proteomics in colonic mucosa of sub-health people with constipation. Progress in Biochemistry and Biophysics. 11:1043–1051. 2011.In Chinese. View Article : Google Scholar

9 

Million M, Taché Y and Anton P: Susceptibility of Lewis and Fischer rats to stress-induced worsening of TNB-colitis: Protective role of brain CRF. Am J Physiol. 276:G1027–G1036. 1999.PubMed/NCBI

10 

Csabafi K, Jászberényi M, Bagosi Z, Lipták N and Telegdy G: Effects of kisspeptin-13 on the hypothalamic-pituitary-adrenal axis, thermoregulation, anxiety and locomotor activity in rats. Behav Brain Res. 241:56–61. 2013. View Article : Google Scholar

11 

Hall CS: Emotional behavior in the rat. I. Defacation and urination as measures of individual differences in emotionality. J Comp Psychol. 18:385–403. 1934. View Article : Google Scholar

12 

Whimbey AE and Denenberg VH: Two independent behavioral dimensions in open-field performance. J Comp Physiol Psychol. 63:500–504. 1967. View Article : Google Scholar : PubMed/NCBI

13 

Flint J, Corley R, DeFries JC, Fulker DW, Gray JA, Miller S and Collins AC: A simple genetic basis for a complex psychological trait in laboratory mice. Science. 269:1432–1435. 1995. View Article : Google Scholar : PubMed/NCBI

14 

Toker H, Ozdemir H, Balci H and Ozer H: N-acetylcysteine decreases alveolar bone loss on experimental periodontitis in streptozotocin-induced diabetic rats. J Periodontal Res. 47:793–799. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Füzi M, Palicz Z, Vincze J, Cseri J, Szombathy Z, Kovács I, Oláh A, Szentesi P, Kertai P, Paragh G and Csernoch L: Fluvastatin-induced alterations of skeletal muscle function in hypercholesterolaemic rats. J Muscle Res Cell Motil. 32:391–401. 2012. View Article : Google Scholar

16 

Luo J and Huang L: Research progress in the identification and classification of sub-health state. Chinese Medicine Modern Distance Education of China. 13:154–156. 2015.In Chinese.

17 

Ferrick DA, Schrenzel MD, Mulvania T, Hsieh B, Ferlin WG and Lepper H: Differential production of interferon-gamma and interleukin-4 in response to Th1- and Th2-stimulating pathogens by gamma delta T cells in vivo. Nature. 373:255–257. 1995. View Article : Google Scholar : PubMed/NCBI

18 

Yao XY, Wang HM, Li DJ, Yuan MM, Wang XL, Yu M, Wang MY, Zhu Y and Meng Y: Inoculation of Lactobacillus expressing hCG beta in the vagina induces an anti-hCG beta antibody response in murine vaginal mucosa. J Reprod Immunol. 63:111–122. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Liu G, Zhong J, Ni J, Chen M, Xiao H and Huan L: Characteristics of the bovicin HJ50 gene cluster in Streptococcus bovis HJ50. Microbiology. 155:584–593. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Choleris E, Thomas AW, Kavaliers M and Prato FS: A detailed ethological analysis of the mouse open field test: Effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field. Neurosci Biobehav Rev. 25:235–260. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Prut L and Belzung C: The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: A review. Eur J Pharmacol. 463:3–33. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Sharma S, Coombs S, Patton P and Burt de Perera T: The function of wall-following behaviors in the Mexican blind cavefish and a sighted relative, the Mexican tetra (Astyanax). J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 195:225–240. 2009. View Article : Google Scholar

23 

Belzung C and Griebel G: Measuring normal and pathological anxiety-like behaviour in mice: A review. Behav Brain Res. 125:141–149. 2001. View Article : Google Scholar : PubMed/NCBI

24 

McGhee JR and Fujihashi K: Inside the mucosal immune system. PLoS Biol. 10:e1001397PubMed/NCBI

25 

Kamiya S, Nagino M, Kanazawa H, Komatsu S, Mayumi T, Takagi K, Asahara T, Nomoto K, Tanaka R and Nimura Y: The value of bile replacement during external biliary drainage: An analysis of intestinal permeability, integrity and microflora. Ann Surg. 239:510–517. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Luk GD, Bayless TM and Baylin SB: Plasma postheparin diamine oxidase. Sensitive provocative test for quantitating length of acute intestinal mucosal injury in the rat. J Clin Invest. 71:1308–1315. 1983. View Article : Google Scholar : PubMed/NCBI

27 

Smith SM, Eng RH, Campos JM and Chmel H: D-lactic acid measurements in the diagnosis of bacterial infections. J Clin Microbiol. 27:385–388. 1989.PubMed/NCBI

28 

Gardino AK and Yaffe MB: 14-3-3 proteins as signaling integration points for cell cycle control and apoptosis. Semin Cell Dev Biol. 22:688–695. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Masters SC and Fu H: 14-3-3 proteins mediate an essential anti-apoptotic signal. J Biol Chem. 276:45193–45200. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Bharadwaj S, Vishnubhotla R, Shan S, Chauhan C, Cho M and Glover SC: Higher molecular weight polyethylene glycol increases cell proliferation while improving barrier function in an in vitro colon cancer model. J Biomed Biotechnol. 2011:5874702011. View Article : Google Scholar : PubMed/NCBI

31 

Bojarski C, Bendfeldt K, Gitter AH, Mankertz J, Fromm M, Wagner S, Riecken EO and Schulzke JD: Apoptosis and intestinal barrier function. Ann N Y Acad Sci. 915:270–274. 2000. View Article : Google Scholar

32 

Rachetti AL, Arida RM, Patti CL, Zanin KA, Fernades-Santos L, Frussa-Filho R, Gomes da Silva S, Scorza FA and Cysneiros RM: Fish oil supplementation and physical exercise program: Distinct effects on different memory tasks. Behav Brain Res. 237:283–289. 2013. View Article : Google Scholar

33 

Jirillo E, Jirillo F and Magrone T: Healthy effects exerted by prebiotics, probiotics and symbiotics with special reference to their impact on the immune system. Int J Vitam Nutr Res. 82:200–208. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Korhan EA, Uyar M, Eyigör C, Hakverdioğlu Yönt G, Çelik S and Khorshid L: The effects of music therapy on pain in patients with neuropathic pain. Pain Manag Nurs. 15:306–314. 2014. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Feng S, Liu W, Zuo S, Xie T, Deng H, Zhang Q and Zhong B: Impaired function of the intestinal barrier in a novel sub-health rat model. Mol Med Rep 13: 3459-3465, 2016.
APA
Feng, S., Liu, W., Zuo, S., Xie, T., Deng, H., Zhang, Q., & Zhong, B. (2016). Impaired function of the intestinal barrier in a novel sub-health rat model. Molecular Medicine Reports, 13, 3459-3465. https://doi.org/10.3892/mmr.2016.4978
MLA
Feng, S., Liu, W., Zuo, S., Xie, T., Deng, H., Zhang, Q., Zhong, B."Impaired function of the intestinal barrier in a novel sub-health rat model". Molecular Medicine Reports 13.4 (2016): 3459-3465.
Chicago
Feng, S., Liu, W., Zuo, S., Xie, T., Deng, H., Zhang, Q., Zhong, B."Impaired function of the intestinal barrier in a novel sub-health rat model". Molecular Medicine Reports 13, no. 4 (2016): 3459-3465. https://doi.org/10.3892/mmr.2016.4978
Copy and paste a formatted citation
x
Spandidos Publications style
Feng S, Liu W, Zuo S, Xie T, Deng H, Zhang Q and Zhong B: Impaired function of the intestinal barrier in a novel sub-health rat model. Mol Med Rep 13: 3459-3465, 2016.
APA
Feng, S., Liu, W., Zuo, S., Xie, T., Deng, H., Zhang, Q., & Zhong, B. (2016). Impaired function of the intestinal barrier in a novel sub-health rat model. Molecular Medicine Reports, 13, 3459-3465. https://doi.org/10.3892/mmr.2016.4978
MLA
Feng, S., Liu, W., Zuo, S., Xie, T., Deng, H., Zhang, Q., Zhong, B."Impaired function of the intestinal barrier in a novel sub-health rat model". Molecular Medicine Reports 13.4 (2016): 3459-3465.
Chicago
Feng, S., Liu, W., Zuo, S., Xie, T., Deng, H., Zhang, Q., Zhong, B."Impaired function of the intestinal barrier in a novel sub-health rat model". Molecular Medicine Reports 13, no. 4 (2016): 3459-3465. https://doi.org/10.3892/mmr.2016.4978
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team