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Article

Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway

  • Authors:
    • Ling‑Yu Qiu
    • He‑Ping Chen
    • Yu‑Feng Yan
    • Yuan‑Yuan Li
    • Huan Wang
    • Zhang‑Ping Liao
    • Qi‑Ren Huang
  • View Affiliations / Copyright

    Affiliations: The Key Laboratory of Basic Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 3597-3603
    |
    Published online on: March 7, 2016
       https://doi.org/10.3892/mmr.2016.4984
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Abstract

Sasanquasaponin (SQS) is an active component of Camellia oleifera Abel. A recent study by our group demonstrated that SQS was able to inhibit ischemia/reperfusion‑induced elevation of the intracellular chloride ion concentration ([Cl‑]i) and exerted cardioprotective effects; however, the underlying intracellular signal transduction mechanisms have yet to be elucidated. As protein kinase C ε (PKCε) is able to mediate Cl‑ homeostasis, the present study investigated its possible involvement in the effects of SQS on cardiomyocytes subjected to ischemia/reperfusion injury. Cardiomyocytes were pre‑treated with or without SQS or SQS plus εV1‑2, a selective PKCε inhibitor, followed by simulated ischemia/reperfusion (sI/R). The effects on cell viability, PKCε phosphorylation levels, [Cl‑]i, mitochondrial membrane potential and reactive oxygen species (ROS) production were assessed using an MTS assay, western blot analysis, colorimetric assays and flow cytometry. The results revealed that treatment with SQS prior to sI/R increased the viability of cardiomyocytes, and efficiently attenuated lactate dehydrogenase and creatine phosphokinase release induced by sI/R. In addition, SQS promoted PKCε phosphorylation and inhibited sI/R‑induced elevation of [Cl‑]i, paralleled by the attenuation of mitochondrial membrane potential loss and ROS generation. However, when the cardiomyocytes were treated with εV1‑2 prior to SQS pre‑conditioning, the cardioprotection induced by SQS was reduced and the inhibitory effects of SQS on sI/R‑induced elevation of [Cl‑]i, production of ROS and loss of mitochondrial membrane potential were also attenuated. These findings indicated that SQS may inhibit sI/R‑induced elevation of [Cl‑]i through the PKCε signaling pathway to elicit cardioprotection in cultured cardiomyocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Qiu LY, Chen HP, Yan YF, Li YY, Wang H, Liao ZP and Huang QR: Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway. Mol Med Rep 13: 3597-3603, 2016.
APA
Qiu, L., Chen, H., Yan, Y., Li, Y., Wang, H., Liao, Z., & Huang, Q. (2016). Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway. Molecular Medicine Reports, 13, 3597-3603. https://doi.org/10.3892/mmr.2016.4984
MLA
Qiu, L., Chen, H., Yan, Y., Li, Y., Wang, H., Liao, Z., Huang, Q."Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway". Molecular Medicine Reports 13.4 (2016): 3597-3603.
Chicago
Qiu, L., Chen, H., Yan, Y., Li, Y., Wang, H., Liao, Z., Huang, Q."Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway". Molecular Medicine Reports 13, no. 4 (2016): 3597-3603. https://doi.org/10.3892/mmr.2016.4984
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu LY, Chen HP, Yan YF, Li YY, Wang H, Liao ZP and Huang QR: Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway. Mol Med Rep 13: 3597-3603, 2016.
APA
Qiu, L., Chen, H., Yan, Y., Li, Y., Wang, H., Liao, Z., & Huang, Q. (2016). Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway. Molecular Medicine Reports, 13, 3597-3603. https://doi.org/10.3892/mmr.2016.4984
MLA
Qiu, L., Chen, H., Yan, Y., Li, Y., Wang, H., Liao, Z., Huang, Q."Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway". Molecular Medicine Reports 13.4 (2016): 3597-3603.
Chicago
Qiu, L., Chen, H., Yan, Y., Li, Y., Wang, H., Liao, Z., Huang, Q."Sasanquasaponin promotes cellular chloride efflux and elicits cardioprotection via the PKCε pathway". Molecular Medicine Reports 13, no. 4 (2016): 3597-3603. https://doi.org/10.3892/mmr.2016.4984
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