Open Access

Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment

  • Authors:
    • Ji Yoon Lee
    • A‑Reum Han
    • Sung‑Eun Lee
    • Woo‑Sung Min
    • Hee‑Je Kim
  • View Affiliations

  • Published online on: March 18, 2016     https://doi.org/10.3892/mmr.2016.5009
  • Pages: 3849-3857
  • Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Podoplanin+ cells are indispensable in the tumor microenvironment. Increasing evidence suggests that podoplanin may support the growth and metastasis of solid tumors; however, to the best of our knowledge no studies have determined whether or not podoplanin serves a supportive role in acute myeloid leukemia (AML). The effects of co‑culture with podoplanin+ cells on the cellular activities of the leukemic cells, such as apoptosis and cell proliferation, in addition to the expression of podoplanin in leukemic cells, were investigated. Due to the fact that genetic abnormalities are the primary cause of leukemogenesis, the overexpression of the fibromyalgia‑like tyrosine kinase‑3 gene in colony forming units was also examined following cell sorting. Podoplanin+ cells were found to play a protective role against apoptosis in leukemic cells and to promote cell proliferation. Tumor‑associated antigens, including Wilms' tumor gene 1 and survivin, were increased when leukemic cells were co‑cultured with podoplanin+ cells. In combination, the present results also suggest that podoplanin+ cells can function as stromal cells for blast cell retention in the AML tumor microenvironment.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lee JY, Han AR, Lee SE, Min WS and Kim HJ: Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment. Mol Med Rep 13: 3849-3857, 2016
APA
Lee, J.Y., Han, A., Lee, S., Min, W., & Kim, H. (2016). Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment. Molecular Medicine Reports, 13, 3849-3857. https://doi.org/10.3892/mmr.2016.5009
MLA
Lee, J. Y., Han, A., Lee, S., Min, W., Kim, H."Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment". Molecular Medicine Reports 13.5 (2016): 3849-3857.
Chicago
Lee, J. Y., Han, A., Lee, S., Min, W., Kim, H."Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment". Molecular Medicine Reports 13, no. 5 (2016): 3849-3857. https://doi.org/10.3892/mmr.2016.5009