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Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment

  • Authors:
    • Ji Yoon Lee
    • A‑Reum Han
    • Sung‑Eun Lee
    • Woo‑Sung Min
    • Hee‑Je Kim
  • View Affiliations / Copyright

    Affiliations: Leukemia Research Institute, Catholic Blood and Marrow Transplantation Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137‑701, Republic of Korea, Department of Hematology, Catholic Blood and Marrow Transplantation Center, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul 137‑701, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3849-3857
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    Published online on: March 18, 2016
       https://doi.org/10.3892/mmr.2016.5009
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Abstract

Podoplanin+ cells are indispensable in the tumor microenvironment. Increasing evidence suggests that podoplanin may support the growth and metastasis of solid tumors; however, to the best of our knowledge no studies have determined whether or not podoplanin serves a supportive role in acute myeloid leukemia (AML). The effects of co‑culture with podoplanin+ cells on the cellular activities of the leukemic cells, such as apoptosis and cell proliferation, in addition to the expression of podoplanin in leukemic cells, were investigated. Due to the fact that genetic abnormalities are the primary cause of leukemogenesis, the overexpression of the fibromyalgia‑like tyrosine kinase‑3 gene in colony forming units was also examined following cell sorting. Podoplanin+ cells were found to play a protective role against apoptosis in leukemic cells and to promote cell proliferation. Tumor‑associated antigens, including Wilms' tumor gene 1 and survivin, were increased when leukemic cells were co‑cultured with podoplanin+ cells. In combination, the present results also suggest that podoplanin+ cells can function as stromal cells for blast cell retention in the AML tumor microenvironment.
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Copy and paste a formatted citation
Spandidos Publications style
Lee JY, Han AR, Lee SE, Min WS and Kim HJ: Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment. Mol Med Rep 13: 3849-3857, 2016.
APA
Lee, J.Y., Han, A., Lee, S., Min, W., & Kim, H. (2016). Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment. Molecular Medicine Reports, 13, 3849-3857. https://doi.org/10.3892/mmr.2016.5009
MLA
Lee, J. Y., Han, A., Lee, S., Min, W., Kim, H."Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment". Molecular Medicine Reports 13.5 (2016): 3849-3857.
Chicago
Lee, J. Y., Han, A., Lee, S., Min, W., Kim, H."Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment". Molecular Medicine Reports 13, no. 5 (2016): 3849-3857. https://doi.org/10.3892/mmr.2016.5009
Copy and paste a formatted citation
x
Spandidos Publications style
Lee JY, Han AR, Lee SE, Min WS and Kim HJ: Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment. Mol Med Rep 13: 3849-3857, 2016.
APA
Lee, J.Y., Han, A., Lee, S., Min, W., & Kim, H. (2016). Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment. Molecular Medicine Reports, 13, 3849-3857. https://doi.org/10.3892/mmr.2016.5009
MLA
Lee, J. Y., Han, A., Lee, S., Min, W., Kim, H."Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment". Molecular Medicine Reports 13.5 (2016): 3849-3857.
Chicago
Lee, J. Y., Han, A., Lee, S., Min, W., Kim, H."Co-culture with podoplanin+ cells protects leukemic blast cells with leukemia-associated antigens in the tumor microenvironment". Molecular Medicine Reports 13, no. 5 (2016): 3849-3857. https://doi.org/10.3892/mmr.2016.5009
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