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Article

MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells

  • Authors:
    • Shumei Jiang
    • Renben Wang
    • Hongjiang Yan
    • Linzhi Jin
    • Xue Dou
    • Dong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Jinan, Shandong 250117, P.R. China
  • Pages: 4101-4107
    |
    Published online on: March 18, 2016
       https://doi.org/10.3892/mmr.2016.5010
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Abstract

Aberrant microRNA (miRNA) expression in cancer affects the transcription of target genes, and profoundly influences cancer‑associated signaling pathways. Radiation resistance is a major problem encountered in the treatment of cancer. The present study aimed to investigate the role of miRNA (miR)‑21 in the development of radiation resistance in non‑small cell lung cancer cells. A radiation‑resistant cell line was generated from A549 cells. Significant upregulation of miR‑21 was detected in the radioresistant cancer cells, as compared with the radiosensitive cells, and overexpression of miR‑21 rendered A549 parental cells resistant to radiation. In addition, glycolysis was increased in the radioresistant cells, as compared with the sensitive cells. Furthermore, hypoxia‑inducible factor‑1α (HIF1α) was upregulated by miR‑21 in radioresistant cells, resulting in promotion of the key enzymes of glycolysis. Inhibition of HIF1α by small interfering RNA suppressed glycolysis and resensitized the cancer cells to radiation, whereas the recovery of HIF1α in miR‑21‑inhibited radioresistant cells resulted in recovery of radioresistance. In conclusion, the present study suggested that miR‑21 may modulate radioresistance through the upregulation of HIF1α. These results may provide a novel perspective on miRNA for the development of anti-radioresistance drugs.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang S, Wang R, Yan H, Jin L, Dou X and Chen D: MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells. Mol Med Rep 13: 4101-4107, 2016.
APA
Jiang, S., Wang, R., Yan, H., Jin, L., Dou, X., & Chen, D. (2016). MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells. Molecular Medicine Reports, 13, 4101-4107. https://doi.org/10.3892/mmr.2016.5010
MLA
Jiang, S., Wang, R., Yan, H., Jin, L., Dou, X., Chen, D."MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells". Molecular Medicine Reports 13.5 (2016): 4101-4107.
Chicago
Jiang, S., Wang, R., Yan, H., Jin, L., Dou, X., Chen, D."MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells". Molecular Medicine Reports 13, no. 5 (2016): 4101-4107. https://doi.org/10.3892/mmr.2016.5010
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang S, Wang R, Yan H, Jin L, Dou X and Chen D: MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells. Mol Med Rep 13: 4101-4107, 2016.
APA
Jiang, S., Wang, R., Yan, H., Jin, L., Dou, X., & Chen, D. (2016). MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells. Molecular Medicine Reports, 13, 4101-4107. https://doi.org/10.3892/mmr.2016.5010
MLA
Jiang, S., Wang, R., Yan, H., Jin, L., Dou, X., Chen, D."MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells". Molecular Medicine Reports 13.5 (2016): 4101-4107.
Chicago
Jiang, S., Wang, R., Yan, H., Jin, L., Dou, X., Chen, D."MicroRNA-21 modulates radiation resistance through upregulation of hypoxia-inducible factor-1α-promoted glycolysis in non-small cell lung cancer cells". Molecular Medicine Reports 13, no. 5 (2016): 4101-4107. https://doi.org/10.3892/mmr.2016.5010
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