Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2016 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2016 Volume 13 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect

  • Authors:
    • Yaxiang Jin
    • Xinyu Hu
    • Yan Zhang
    • Tianji Liu
  • View Affiliations / Copyright

    Affiliations: Physical Examination Center, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Department of Clinical Medicine, Changchun Medical College, Changchun, Jilin 130021, P.R. China, Department of Breast Surgery, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China
  • Pages: 3985-3992
    |
    Published online on: March 21, 2016
       https://doi.org/10.3892/mmr.2016.5026
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the protective effect of purified polysaccharides from Tremella fuciformis against glutamate-induced cytotoxicity in differentiated PC12 (DPC12) cells. The aqueous extract of Tremella fuciformis was purified using a DEAE-52 cellulose anion exchange column and a Sepharose G-100 column, respectively. A fraction termed TL04 with a 2,033 kDa molecular weight was obtained. The backbone of TL04 is composed of (1→2)‑ and (1→4)-linked-mannose and (1→3)-linked-glucans. Results revealed that TL04 treatment improved cell viability and suppressed reactive oxygen species accumulation, lactose dehydrogenase release and caspase-3 activity, and ameliorated mitochondrial abnormal alteration caused by glutamate. TL04 pretreatment enhanced the level of B‑cell lymphoma 2 (Bcl‑2), and suppressed Bax expression and cytochrome c (Cyto C) release in glutamate-treated cells. Exposure to glutamate strongly increased the activity of caspase‑8, caspase‑9 and caspase‑3, which were significantly reversed by TL04 pretreatment. The presence of Ac-DEVD-CHO (a caspase-3 inhibitor) markedly enhanced the potency of TL04 in improving the viability of glutamate-exposed DPC12 cells. Collectively, the results demonstrated that the purified polysaccharides separated from Tremella fuciformis (TL04) possess a neuroprotective effect against glutamate-induced DPC12 cell damage predominantly through the caspase-dependent mitochondrial pathway. The present study provides an experimental foundation supporting purified TL04 as a potential therapeutic agent for neurodegenerative diseases.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Traynelis SF, Wollmuth LP, McBain CJ, Menniti FS, Vance KM, Ogden KK, Hansen KB, Yuan H, Myers SJ and Dingledine R: Glutamate receptor ion channels: Structure, regulation and function. Pharmacol Rev. 62:405–496. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Reynolds IJ and Hastings TG: Glutamate induces the production of reactive oxygen species in cultured forebrain neurons following NMDA receptor activation. J Neurosci. 15:3318–3327. 1995.PubMed/NCBI

3 

Akaishi T, Nakazawa K, Sato K, Saito H, Ohno Y and Ito Y: Hydrogen peroxide modulates whole cell Ca2+ currents through L-type channels in cultured rat dentate granule cells. Neurosci Lett. 356:25–28. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Chamoun R, Suki D, Gopinath SP, Goodman JC and Robertson C: Role of extracellular glutamate measured by cerebral microdialysis in severe traumatic brain injury. J Neurosurg. 113:564–570. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Coyle JT and Puttfarcken P: Oxidative stress, glutamate and neurodegenerative disorders. Science. 262:689–695. 1993. View Article : Google Scholar : PubMed/NCBI

6 

El-Najjar N, Chatila M, Moukadem H, Vuorela H, Ocker M, Gandesiri M, Schneider-Stock R and Gali-Muhtasib H: Reactive oxygen species mediate thymoquinone-induced apoptosis and activate ERK and JNK signaling. Apoptosis. 15:183–195. 2010. View Article : Google Scholar

7 

Adams JM and Cory S: The Bcl-2 protein family: Arbiters of cell survival. Science. 281:1322–1326. 1998. View Article : Google Scholar : PubMed/NCBI

8 

Tsujimoto Y and Shimizu S: Role of the mitochondrial membrane permeability transition in cell death. Apoptosis. 12:835–840. 2007. View Article : Google Scholar

9 

Simon HU, Haj-Yehia A and Levi-Schaffer F: Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis. 5:415–418. 2000. View Article : Google Scholar

10 

Ricci JE, Gottlieb RA and Green DR: Caspase-mediated loss of mitochondrial function and generation of reactive oxygen species during apoptosis. J Cell Biol. 160:65–75. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Methacanon P, Madla S, Kirtikara K and Prasitsil M: Structural elucidation of bioactive fungi-derived polymers. Carbohydrate Polymers. 60:199–203. 2005. View Article : Google Scholar

12 

Zeng Y, Han Z, Qiu P, Zhou Z, Tang Y, Zhao Y, Zheng S, Xu C, Zhang X, Yin P, et al: Salinity-induced anti-angiogenesis activities and structural changes of the polysaccharides from cultured cordyceps militaris. PLoS One. 9:e1038802014. View Article : Google Scholar : PubMed/NCBI

13 

Habijanic J, Berovic M, Boh B, Plankl M and Wraber B: Submerged cultivation of Ganoderma lucidum and the effects of its polysaccharides on the production of human cytokines TNF-α, IL-12, IFN-γ, IL-2, IL-4, IL-10 and IL-17. N Biotechnol. 32:85–95. 2015. View Article : Google Scholar

14 

Ren M, Ye L, Hao X, Ren Z, Ren S, Xu K and Li J: Polysaccharides from Tricholoma matsutake and Lentinus edodes enhance 5-fluorouracil-mediated H22 cell growth inhibition. J Tradit Chin Med. 34:309–316. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Gao Q, Seljelid R, Chen H and Jiang R: Characterisation of acidic heteroglycans from Tremella fuciformis Berk with cytokine stimulating activity. Carbohydr Res. 288:135–142. 1996. View Article : Google Scholar : PubMed/NCBI

16 

Ukai S, Kiriki H, Nagai K and Kiho T: Synthesis and antitumor activities of conjugates of mitomycin C-polysaccharide from Tremella fuciformis. Yakugaku Zasshi. 112:663–668. 1992.In Japanese. PubMed/NCBI

17 

Ukai S, Kiho T, Hara C, Kuruma I and Tanaka Y: Polysaccharides in fungi. XIV. Anti-inflammatory effect of the polysaccharides from the fruit bodies of several fungi. J Pharmacobiodyn. 6:983–990. 1983. View Article : Google Scholar : PubMed/NCBI

18 

Park HJ, Shim HS, Ahn YH, Kim KS, Park KJ, Choi WK, Ha HC, Kang JI, Kim TS, Yeo IH, et al: Tremella fuciformis enhances the neurite outgrowth of PC12 cells and restores trimethyltin-induced impairment of memory in rats via activation of CREB transcription and cholinergic systems. Behav Brain Res. 229:82–90. 2012. View Article : Google Scholar

19 

Xu W, Shen X, Yang F, Han Y, Li R, Xue D and Jiang C: Protective effect of polysaccharides isolated from Tremella fuciformis against radiation-induced damage in mice. J Radiat Res. 53:353–360. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Zhang Z, Wang X, Zhao M and Qi H: Free-radical degradation by Fe2+/Vc/H2O2 and antioxidant activity of polysaccharide from Tremella fuciformis. Carbohydr Polym. 112:578–582. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Du L, Song J, Wang H, Li P, Yang Z, Meng L, Meng F, Lu J and Teng L: Optimization of the fermentation medium for Paecilomyces tenuipes N45 using statistical approach. African Journal of Microbiology Research. 6:6130–6141. 2012. View Article : Google Scholar

22 

Yan H, Zhu D, Xu D, Wu J and Bian X: A study on Cordyceps militaris polysaccharide purification, composition and activity analysis. African Journal of Biotechnology. 7:4004–4009. 2008.

23 

Dong Y, Hu S, Liu C, Meng Q, Song J, Lu J, Cheng Y, Gao C, Liu Y, Wang D and Teng L: Purification of polysaccharides from Cordyceps militaris and their anti-hypoxic effect. Mol Med Rep. 11:1312–1317. 2015.

24 

Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55–63. 1983. View Article : Google Scholar : PubMed/NCBI

25 

Yang CL, Chik SC, Li JC, Cheung BK and Lau AS: Identification of the bioactive constituent and its mechanisms of action in mediating the anti-inflammatory effects of black cohosh and related Cimicifuga species on human primary blood macrophages. J Med Chem. 52:6707–6715. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Santhiya D, Subramanian S and Natarajan K: Surface chemical studies on sphalerite and galena using extracellular polysaccharides isolated from Bacillus polymyxa. J Colloid Interface Sci. 256:237–248. 2002. View Article : Google Scholar

27 

Pielesz A: Vibrational spectroscopy and electrophoresis as a 'golden means' in monitoring of polysaccharides in medical plant and gels. Spectrochim Acta A Mol Biomol Spectrosc. 93:63–69. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Du XJ, Zhang JS, Yang Y, Tang QJ, Jia W and Pan YJ: Purification, chemical modification and immunostimulating activity of polysaccharides from Tremella aurantialba fruit bodies. J Zhejiang Univ Sci B. 11:437–442. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Lee CS, Kim YJ, Lee MS, Han ES and Lee SJ: 18beta-Glycyrrhetinic acid induces apoptotic cell death in SiHa cells and exhibits a synergistic effect against antibiotic anti-cancer drug toxicity. Life Sci. 83:481–489. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Raisova M, Hossini AM, Eberle J, Riebeling C, Wieder T, Sturm I, Daniel PT, Orfanos CE and Geilen CC: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis. J Invest Dermatol. 117:333–340. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Kroemer G, Dallaporta B and Resche-Rigon M: The mitochondrial death/life regulator in apoptosis and necrosis. Annu Rev Physiol. 60:619–642. 1998. View Article : Google Scholar : PubMed/NCBI

32 

Circu ML and Aw TY: Reactive oxygen species, cellular redox systems and apoptosis. Free Radic Biol Med. 48:749–762. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Zhang L and Zhang S: Modulating Bcl-2 family proteins and caspase-3 in induction of apoptosis by paeoniflorin in human cervical cancer cells. Phytother Res. 25:1551–1557. 2011. View Article : Google Scholar : PubMed/NCBI

34 

Hu Q, Wu D, Chen W, Yan Z and Shi Y: Proteolytic processing of the caspase-9 zymogen is required for apoptosome-mediated activation of caspase-9. J Biol Chem. 288:15142–15147. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Boatright KM, Renatus M, Scott FL, Sperandio S, Shin H, Pedersen IM, Ricci JE, Edris WA, Sutherlin DP, Green DR and Salvesen GS: A unified model for apical caspase activation. Mol Cell. 11:529–541. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Fulda S and Vucic D: Targeting IAP proteins for therapeutic intervention in cancer. Nat Rev Drug Discov. 11:109–124. 2012. View Article : Google Scholar : PubMed/NCBI

37 

Yuan S and Akey CW: Apoptosome structure, assembly and procaspase activation. Structure. 21:501–515. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Jin Z and El-Deiry WS: Overview of cell death signaling pathways. Cancer Biol Ther. 4:139–163. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jin Y, Hu X, Zhang Y and Liu T: Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect. Mol Med Rep 13: 3985-3992, 2016.
APA
Jin, Y., Hu, X., Zhang, Y., & Liu, T. (2016). Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect. Molecular Medicine Reports, 13, 3985-3992. https://doi.org/10.3892/mmr.2016.5026
MLA
Jin, Y., Hu, X., Zhang, Y., Liu, T."Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect". Molecular Medicine Reports 13.5 (2016): 3985-3992.
Chicago
Jin, Y., Hu, X., Zhang, Y., Liu, T."Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect". Molecular Medicine Reports 13, no. 5 (2016): 3985-3992. https://doi.org/10.3892/mmr.2016.5026
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Y, Hu X, Zhang Y and Liu T: Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect. Mol Med Rep 13: 3985-3992, 2016.
APA
Jin, Y., Hu, X., Zhang, Y., & Liu, T. (2016). Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect. Molecular Medicine Reports, 13, 3985-3992. https://doi.org/10.3892/mmr.2016.5026
MLA
Jin, Y., Hu, X., Zhang, Y., Liu, T."Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect". Molecular Medicine Reports 13.5 (2016): 3985-3992.
Chicago
Jin, Y., Hu, X., Zhang, Y., Liu, T."Studies on the purification of polysaccharides separated from Tremella fuciformis and their neuroprotective effect". Molecular Medicine Reports 13, no. 5 (2016): 3985-3992. https://doi.org/10.3892/mmr.2016.5026
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team