Lazaroid U83836E protects the heart against ischemia reperfusion injury via inhibition of oxidative stress and activation of PKC

  • Authors:
    • Li‑Na Lai
    • Xiao‑Jing Zhang
    • Xiao‑Yi Zhang
    • Li‑Hua Song
    • Chun‑Hua Guo
    • Jing‑Wen Lei
    • Xiao‑Liang Song
  • View Affiliations

  • Published online on: March 21, 2016     https://doi.org/10.3892/mmr.2016.5030
  • Pages: 3993-4000
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Abstract

Oxidative stress has been demonstrated to be important during myocardial ischemia/reperfusion injury (MIRI). The lazaroid U83836E, which combines the amino functionalities of the 21‑aminosteroids with the antioxidant ring portion of vitamin E, is a reactive oxygen species scavenger. The aim of the current study was to investigate the effect of U83836E on MIRI and its mechanisms of action. Rat hearts were subjected to 30 min ligation of the left anterior descending coronary artery, followed by 2 h reperfusion. The results demonstrated that at 5 mg/kg, U83836E markedly protected cardiac function in ischemia/reperfusion rat models, decreased the malondialdehyde content and creatinine kinase activity, while increasing superoxide dismutase and glutathione peroxidase activity. Additionally, U83836E significantly decreased the histological damage to the myocardium, reduced the area of myocardial infarction in the left ventricle and modified the mitochondrial dysfunction. Furthermore, U83836E enhanced the translocation of protein kinase Cε (PKCε) from the cytoplasm to the membrane. However, the cardioprotective effects of U83836E were reduced in the presence of the PKC inhibitor, chelerythrine (1 mg/kg). Therefore, the results of the present study suggest that U83836E has a potent protective effect against MIRI in rat models through the direct anti‑oxidative stress mechanisms and the activation of PKC signaling.
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May-2016
Volume 13 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lai LN, Zhang XJ, Zhang XY, Song LH, Guo CH, Lei JW and Song XL: Lazaroid U83836E protects the heart against ischemia reperfusion injury via inhibition of oxidative stress and activation of PKC. Mol Med Rep 13: 3993-4000, 2016
APA
Lai, L., Zhang, X., Zhang, X., Song, L., Guo, C., Lei, J., & Song, X. (2016). Lazaroid U83836E protects the heart against ischemia reperfusion injury via inhibition of oxidative stress and activation of PKC. Molecular Medicine Reports, 13, 3993-4000. https://doi.org/10.3892/mmr.2016.5030
MLA
Lai, L., Zhang, X., Zhang, X., Song, L., Guo, C., Lei, J., Song, X."Lazaroid U83836E protects the heart against ischemia reperfusion injury via inhibition of oxidative stress and activation of PKC". Molecular Medicine Reports 13.5 (2016): 3993-4000.
Chicago
Lai, L., Zhang, X., Zhang, X., Song, L., Guo, C., Lei, J., Song, X."Lazaroid U83836E protects the heart against ischemia reperfusion injury via inhibition of oxidative stress and activation of PKC". Molecular Medicine Reports 13, no. 5 (2016): 3993-4000. https://doi.org/10.3892/mmr.2016.5030