The downregulation of miR‑125a‑5p functions as a tumor suppressor by directly targeting MMP‑11 in osteosarcoma

  • Authors:
    • Niyazi Waresijiang
    • Jungang Sun
    • Rewuti Abuduaini
    • Tayier Jiang
    • Wenzheng Zhou
    • Hong Yuan
  • View Affiliations

  • Published online on: April 15, 2016     https://doi.org/10.3892/mmr.2016.5141
  • Pages: 4859-4864
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Abstract

Osteosarcoma is one of the most common primary malignant bone cancers in juveniles and adults. Increasingly, reports indicate that microRNAs (miRNAs) may provide novel therapeutic targets for cancer treatment. The aim of the present study was to investigate the expression of miR‑125a‑5p and to identify its functional significance in osteosarcoma. This indicated that miR‑125a‑5p was downregulated in osteosarcoma tissue and cell lines using reverse transcription‑quantitative polymerase chain reaction. Following transfection with miR‑125a‑5p mimics or the negative control, cell migration, invasion and epithelial‑mesenchymal transition (EMT) assays were conducted in osteosarcoma cells. These results indicated that the overexpression of miR‑125a‑5p resulted in inhibited osteosarcoma cell migration, invasion and EMT in vitro. Furthermore, mechanistic studies showed that matrix metallopeptidase‑11 (MMP‑11), was a direct target of miR‑125a‑5p in osteosarcoma. Taken together, the data demonstrate that miR‑125a‑5p functions as a tumor suppressor gene and serves an important role in inhibiting osteosarcoma cell migration, invasion and EMT by targeting MMP‑11.
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June-2016
Volume 13 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Waresijiang N, Sun J, Abuduaini R, Jiang T, Zhou W and Yuan H: The downregulation of miR‑125a‑5p functions as a tumor suppressor by directly targeting MMP‑11 in osteosarcoma. Mol Med Rep 13: 4859-4864, 2016
APA
Waresijiang, N., Sun, J., Abuduaini, R., Jiang, T., Zhou, W., & Yuan, H. (2016). The downregulation of miR‑125a‑5p functions as a tumor suppressor by directly targeting MMP‑11 in osteosarcoma. Molecular Medicine Reports, 13, 4859-4864. https://doi.org/10.3892/mmr.2016.5141
MLA
Waresijiang, N., Sun, J., Abuduaini, R., Jiang, T., Zhou, W., Yuan, H."The downregulation of miR‑125a‑5p functions as a tumor suppressor by directly targeting MMP‑11 in osteosarcoma". Molecular Medicine Reports 13.6 (2016): 4859-4864.
Chicago
Waresijiang, N., Sun, J., Abuduaini, R., Jiang, T., Zhou, W., Yuan, H."The downregulation of miR‑125a‑5p functions as a tumor suppressor by directly targeting MMP‑11 in osteosarcoma". Molecular Medicine Reports 13, no. 6 (2016): 4859-4864. https://doi.org/10.3892/mmr.2016.5141