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June-2016 Volume 13 Issue 6

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells

  • Authors:
    • Fang Wang
    • Jincheng Xu
    • Hao Liu
    • Zhe Liu
    • Fei Xia
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Oncology, First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Biochemical Pharmacology, Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China
  • Pages: 4671-4676
    |
    Published online on: April 15, 2016
       https://doi.org/10.3892/mmr.2016.5143
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Abstract

In recent years, population-based studies and retrospective analyses of clinical studies have shown that metformin treatment is associated with reduced cancer incidence and a decrease in cancer‑associated mortality. However, its mechanism of action remains to be fully understood. The present study demonstrates the effects of metformin on KB human oral cancer cells and explores the role of myeloid cell leukaemia‑1 (Mcl‑1) in metformin‑induced mitochondria‑dependent cellular apoptosis. It was demonstrated that metformin exposure caused significant suppression of KB cell proliferation and induced cell death. Furthermore, metformin induced apoptosis through the downregulation of Mcl‑1 in KB human oral cancer cells, and the overexpression of Mcl‑1 in metformin‑treated KB cells significantly increased cell viability. Consistently, Bax and Bim were upregulated in metformin‑treated cells. The results also reveal that microRNA (miR)‑26a expression was markedly increased by metformin. Subsequent to enforced miR‑26a expression in KB cells using miR‑26a mimics, cell viability and the level of Mcl‑1 decreased. These results suggest that the anti‑proliferative effects of metformin in KB cells may result partly from induction of apoptosis by miR-26a-induced downregulation of Mcl-1.
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Copy and paste a formatted citation
Spandidos Publications style
Wang F, Xu J, Liu H, Liu Z and Xia F: Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells. Mol Med Rep 13: 4671-4676, 2016.
APA
Wang, F., Xu, J., Liu, H., Liu, Z., & Xia, F. (2016). Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells. Molecular Medicine Reports, 13, 4671-4676. https://doi.org/10.3892/mmr.2016.5143
MLA
Wang, F., Xu, J., Liu, H., Liu, Z., Xia, F."Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells". Molecular Medicine Reports 13.6 (2016): 4671-4676.
Chicago
Wang, F., Xu, J., Liu, H., Liu, Z., Xia, F."Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells". Molecular Medicine Reports 13, no. 6 (2016): 4671-4676. https://doi.org/10.3892/mmr.2016.5143
Copy and paste a formatted citation
x
Spandidos Publications style
Wang F, Xu J, Liu H, Liu Z and Xia F: Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells. Mol Med Rep 13: 4671-4676, 2016.
APA
Wang, F., Xu, J., Liu, H., Liu, Z., & Xia, F. (2016). Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells. Molecular Medicine Reports, 13, 4671-4676. https://doi.org/10.3892/mmr.2016.5143
MLA
Wang, F., Xu, J., Liu, H., Liu, Z., Xia, F."Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells". Molecular Medicine Reports 13.6 (2016): 4671-4676.
Chicago
Wang, F., Xu, J., Liu, H., Liu, Z., Xia, F."Metformin induces apoptosis by microRNA-26a-mediated downregulation of myeloid cell leukaemia-1 in human oral cancer cells". Molecular Medicine Reports 13, no. 6 (2016): 4671-4676. https://doi.org/10.3892/mmr.2016.5143
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