Preparation and performance of a pH-sensitive cisplatin-loaded magnetic nanomedicine that targets tumor cells via folate receptor mediation

Chen,Shuai‑Jun; Zhang,Hong‑Zheng; Wan,Liang‑Cai; Jiang,Shan‑Shan; Xu,Yi‑Ming; Liu,Fang; Zhang,Tao; Ma,Dong; Xie,Min‑Qiang

doi:10.3892/mmr.2016.5154

June-2016 Volume 13 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

June-2016 Volume 13 Issue 6

Full Size Image

Article Open Access

Preparation and performance of a pH-sensitive cisplatin-loaded magnetic nanomedicine that targets tumor cells via folate receptor mediation

Authors:
- Shuai‑Jun Chen
- Hong‑Zheng Zhang
- Liang‑Cai Wan
- Shan‑Shan Jiang
- Yi‑Ming Xu
- Fang Liu
- Tao Zhang
- Dong Ma
- Min‑Qiang Xie
View Affiliations / Copyright

Affiliations: Department of Otolaryngology Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China, Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, P.R. China

Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5059-5067
|
Published online on: April 21, 2016

https://doi.org/10.3892/mmr.2016.5154
Expand metrics +

Abstract

The present study aimed to prepare cisplatin (CDDP)-loaded magnetic nanoparticles (MNPs), which target folate receptors via a pH-sensitive release system (FA‑PEG‑NH‑N=MNPs‑CDDP). This is of interest for the development of intelligent drug delivery systems that target tumors of the head and neck. The chemical coprecipitation method was used to prepare ferroferric oxide MNPs. These were modified with aldehyde sodium alginate complexed with the chemotherapeutic agent, CDDP on the surface of the nanoparticles. Double hydrazine‑poly(ethylene glycol; PEG) was also prepared by attaching the carboxyl group of hydrazine‑folate on one side of the double hydrazine‑PEG, obtaining folate‑hydrazine‑PEG‑diazenyl. This binds the aldehyde group of sodium alginic acid on the MNP to enclose CDDP, in order that it is sequestered within the carrier. This method obtained a pH‑sensitive, FA‑modified CDDP‑loaded MNP (FA‑PEG‑NH‑N=MNPs‑CDDP), which acts as an intelligent tumor targeting drug delivery system. The mean size of the MNPs was ~10.2±1.5 nm, the mean hydrodynamic diameter detected by laser particle sizing instruments was 176.6±1.1 nm, and the ζ‑potential was ‑20.91±1.76 mV. The CDDP content was 0.773 mg/ml, the iron content was ~1.908 mg/ml and the maximum saturation magnetization was 16.3±0.2 emu/g. The current study produced a pH‑sensitive FA‑modified CDDP‑loaded MNP that is stable and exhibits magnetic responsiveness, which releases CDDP in a low pH environment.

View Figures

View References

1	Kuang Y, Liu J, Liu Z and Zhuo R: Cholesterol-based anionic long-circulating cisplatin liposomes with reduced renal toxicity. Biomaterials. 33:1596–1606. 2012. View Article : Google Scholar
2	Rath KS, Naidu SK, Lata P, Bid HK, Rivera BK, McCann GA, Tierney BJ, Elnaggar AC, Bravo V, Leone G, et al: HO-3867, a safe STAT3 inhibitor, is selectively cytotoxic to ovarian cancer. Cancer Res. 74:2316–2327. 2014. View Article : Google Scholar : PubMed/NCBI
3	Yu S, Wu G, Gu X, Wang J, Wang Y, Gao H and Ma J: Magnetic and pH-sensitive nanoparticles for antitumor drug delivery. Colloids Surf B Biointerfaces. 103:15–22. 2013. View Article : Google Scholar
4	Kolhatkar R, Lote A and Khambati H: Active tumor targeting of nanomaterials using folic acid, transferrin and integrin receptors. Curr Drug Discov Technol. 8:197–206. 2011. View Article : Google Scholar : PubMed/NCBI
5	Xie M, Chen S, Xu X, et al: Preparation of two kinds of super-paramagnetic carriers-supported cis-platinum complexes and the comparison of their characteristics. Chinese Science Bulletin. 51:151–157. 2006. View Article : Google Scholar
6	Hu X, Liu S, Huang Y, Chen X and Jing X: Biodegradable block copolymer-doxorubicin conjugates via different linkages: Preparation, characterization, and in vitro evaluation. Biomacromolecules. 11:2094–2102. 2010. View Article : Google Scholar : PubMed/NCBI
7	Ohguchi Y, Kawano K, Hattori Y and Maitani Y: Selective delivery of folate-PEG-linked, nanoemulsion-loaded aclacinomycin A to KB nasopharyngeal cells and xenograft: Effect of chain length and amount of folate-PEG linker. J Drug Target. 16:660–667. 2008. View Article : Google Scholar : PubMed/NCBI
8	Laurienzo P, Malinconico M, Motta A and Vicinanza A: Synthesis and characterization of a novel alginate-poly(ethylene glycol) graft copolymer. Carbohydr Polym. 62:274–28. 2005. View Article : Google Scholar
9	Liu J, Xie MQ, Zhang T, Zhang HZ and Xu YM: The preparation and characterization of cisplatin magnetic nanomedicine targeted by folic acid molecular. Zhongguo Zuzhi Gongcheng YanjiuYu Linchuang Kangfu. 25:4631–4637. 2011.
10	Nguyen R and Huc I: Optimizing the reversibility of hydrazone formation for dynamic combinatorial chemistry. Chem Commun (Camb). 9:942–943. 2003. View Article : Google Scholar
11	Qiu XX: Determination of ferrous by spectrophotometry in water. Journal of Southwest University for Nationalities (Natural Science Edition). 1:111–113. 2011.In Chinese.
12	Azzam T and Eisenberg A: Control of vesicular morphologies through hydrophobic block length. Angew Chem Int Ed Engl. 45:7443–7447. 2006. View Article : Google Scholar : PubMed/NCBI
13	Avichezer D, Schechter B and Arnon R: Functional polymers in drug delivery: Carrier-supported CDDP (cis-platin) complexes of polycarboxylates - effect on human ovarian carcinoma. React Funct Polym. 36:59–69. 1998. View Article : Google Scholar
14	Jiang M, Eisenberg A, Liu GJ and Zhang X: Macromolecular Self-Assembly. Science Press; Beijing: 2006, In Chinese.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Preparation and performance of a pH-sensitive cisplatin-loaded magnetic nanomedicine that targets tumor cells via folate receptor mediation

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

Figure 10

Figure 11

Figure 12

Figure 13

Related Articles