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Article

Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients

  • Authors:
    • Yanqiu Fang
    • Xiaodan Lu
    • Lei Liu
    • Xiuying Lin
    • Munan Sun
    • Jianhua Fu
    • Shufen Xu
    • Yan Tan
  • View Affiliations / Copyright

    Affiliations: Reproductive Medical Center, Jilin Province People's Hospital, Changchun, Jilin 130021, P.R. China
  • Pages: 5157-5162
    |
    Published online on: April 25, 2016
       https://doi.org/10.3892/mmr.2016.5173
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Abstract

Misregulation of vascular endothelial growth factor A (VEGF‑A) has been implicated in numerous types of ovarian disease, such as polycystic ovarian syndrome, ovarian hyperstimulation syndrome, endometriosis and ovarian cancer. VEGF regulates blood vessel permeability and angiogenesis. In our previous study, VEGF‑regulated gene expression was profiled in the uterus of a transgenic mouse model with repressed VEGF expression, which indicated that VEGF is an important regulator in controlling gene expression in the uterus. The anti‑Müllerian hormone (AMH) is expressed by ovarian granulosa cells (GCs) and acts through its type 2 receptor, AMH receptor 2 (AMHR2). Serum AMH levels are used to predict ovarian reserves and the small antral follicles contribute markedly to the serum AMH level. AMH recruits primordial follicles and inhibits excessive follicular development by follicular stimulating hormone (FSH). However, AMH may be influenced by suppression of gonadotrophin secretion and VEGF inhibition. In the current study, human primary ovarian GCs were isolated from ovarian follicle fluid of in vitro fertilization/intracytoplasmic sperm injection cycles (IVF/ICSI). It was identified that the FSH receptor was consistently expressed in the isolated cells. VEGF‑A treatment stimulated AMHR2 overexpression at the gene and protein levels. In addition, VEGF induced AMHR2 expression on the surface of the isolated GCs from mature follicles. The VEGF treatment was also performed in an ovarian granulosa‑like cell line, KGN. AMH and AMHR2 are co‑expressed in normal GCs; however, as a result of VEGF misregulation, AMHR2 overexpression increases AMH binding, which may attenuate follicular or oocyte maturation. However, the associated function and underlying mechanism requires further investigation.
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Copy and paste a formatted citation
Spandidos Publications style
Fang Y, Lu X, Liu L, Lin X, Sun M, Fu J, Xu S and Tan Y: Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients. Mol Med Rep 13: 5157-5162, 2016.
APA
Fang, Y., Lu, X., Liu, L., Lin, X., Sun, M., Fu, J. ... Tan, Y. (2016). Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients. Molecular Medicine Reports, 13, 5157-5162. https://doi.org/10.3892/mmr.2016.5173
MLA
Fang, Y., Lu, X., Liu, L., Lin, X., Sun, M., Fu, J., Xu, S., Tan, Y."Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients". Molecular Medicine Reports 13.6 (2016): 5157-5162.
Chicago
Fang, Y., Lu, X., Liu, L., Lin, X., Sun, M., Fu, J., Xu, S., Tan, Y."Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients". Molecular Medicine Reports 13, no. 6 (2016): 5157-5162. https://doi.org/10.3892/mmr.2016.5173
Copy and paste a formatted citation
x
Spandidos Publications style
Fang Y, Lu X, Liu L, Lin X, Sun M, Fu J, Xu S and Tan Y: Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients. Mol Med Rep 13: 5157-5162, 2016.
APA
Fang, Y., Lu, X., Liu, L., Lin, X., Sun, M., Fu, J. ... Tan, Y. (2016). Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients. Molecular Medicine Reports, 13, 5157-5162. https://doi.org/10.3892/mmr.2016.5173
MLA
Fang, Y., Lu, X., Liu, L., Lin, X., Sun, M., Fu, J., Xu, S., Tan, Y."Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients". Molecular Medicine Reports 13.6 (2016): 5157-5162.
Chicago
Fang, Y., Lu, X., Liu, L., Lin, X., Sun, M., Fu, J., Xu, S., Tan, Y."Vascular endothelial growth factor induces anti‑Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients". Molecular Medicine Reports 13, no. 6 (2016): 5157-5162. https://doi.org/10.3892/mmr.2016.5173
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