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Article

Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells

  • Authors:
    • Yunfeng Fu
    • Yanan Zhang
    • Meng Gao
    • Lingli Quan
    • Rong Gui
    • Jing Liu
  • View Affiliations / Copyright

    Affiliations: The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, The First Department of Respiratory Medicine, Central Hospital of Zhuzhou, Zhuzhou, Hunan 412000, P.R. China
  • Pages: 394-398
    |
    Published online on: May 11, 2016
       https://doi.org/10.3892/mmr.2016.5249
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Abstract

Alisertib, a potent and selective Aurora kinase A inhibitor, has been demonstrated to exert potent anti-cancer effects in pre-clinical and clinical studies. However, mechanisms of action of alisertib, including the molecular pathways involved in alisertib-induced apoptosis and autophagy of leukemic cells, have remained elusive. The aim of the present study was to investigate the effects of alisertib on cell growth, apoptosis and autophagy and to delineate the possible molecular mechanisms in leukemic cells. Acid phosphatase, MTT and Annexin V/propidium iodide staining assays as well as immunostaining for light chain 3B showed that treatment of the REH leukemia cell line with alisertib exerted potent growth inhibitory effects, and induced apoptosis and autophagy in a dose‑dependent manner. Western blot analysis indicated that these effects may be attributed to the suppression of the activity of the Akt/mammalian target of rapamycin/5'-AMP-dependent kinase/p38 mitogen-activated protein kinase signaling pathways in REH cells. The present study confirmed that alisertib may represent a promising autophagy-inducing drug for the treatment of leukemia and shed light on its molecular mechanism of action.
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Copy and paste a formatted citation
Spandidos Publications style
Fu Y, Zhang Y, Gao M, Quan L, Gui R and Liu J: Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells. Mol Med Rep 14: 394-398, 2016.
APA
Fu, Y., Zhang, Y., Gao, M., Quan, L., Gui, R., & Liu, J. (2016). Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells. Molecular Medicine Reports, 14, 394-398. https://doi.org/10.3892/mmr.2016.5249
MLA
Fu, Y., Zhang, Y., Gao, M., Quan, L., Gui, R., Liu, J."Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells". Molecular Medicine Reports 14.1 (2016): 394-398.
Chicago
Fu, Y., Zhang, Y., Gao, M., Quan, L., Gui, R., Liu, J."Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells". Molecular Medicine Reports 14, no. 1 (2016): 394-398. https://doi.org/10.3892/mmr.2016.5249
Copy and paste a formatted citation
x
Spandidos Publications style
Fu Y, Zhang Y, Gao M, Quan L, Gui R and Liu J: Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells. Mol Med Rep 14: 394-398, 2016.
APA
Fu, Y., Zhang, Y., Gao, M., Quan, L., Gui, R., & Liu, J. (2016). Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells. Molecular Medicine Reports, 14, 394-398. https://doi.org/10.3892/mmr.2016.5249
MLA
Fu, Y., Zhang, Y., Gao, M., Quan, L., Gui, R., Liu, J."Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells". Molecular Medicine Reports 14.1 (2016): 394-398.
Chicago
Fu, Y., Zhang, Y., Gao, M., Quan, L., Gui, R., Liu, J."Alisertib induces apoptosis and autophagy through targeting the AKT/mTOR/AMPK/p38 pathway in leukemic cells". Molecular Medicine Reports 14, no. 1 (2016): 394-398. https://doi.org/10.3892/mmr.2016.5249
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