Open Access

Comparison of catalase immunoreactivity in the hippocampus between young, adult and aged mice and rats

  • Authors:
    • Ji Hyeon Ahn
    • Bai Hui Chen
    • Bich‑Na Shin
    • Tae‑Kyeong Lee
    • Jeong Hwi Cho
    • In Hye Kim
    • Joon Ha Park
    • Jae‑Chul Lee
    • Hyun‑Jin Tae
    • Choong‑Hyun Lee
    • Moo‑Ho Won
    • Yun Lyul Lee
    • Soo Young Choi
    • Seongkweon Hong
  • View Affiliations

  • Published online on: May 18, 2016     https://doi.org/10.3892/mmr.2016.5300
  • Pages: 851-856
  • Copyright: © Ahn et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Catalase (CAT) is an important antioxidant enzyme and is crucial in modulating synaptic plasticity in the brain. In this study, CAT expression as well as neuronal distribution was compared in the hippocampus among young, adult and aged mice and rats. Male ICR mice and Sprague Dawley rats were used at postnatal month (PM) 1, PM 6 and PM 24 as the young, adult and aged groups, respectively (n=14/group). CAT expression was examined by immunohistochemistry and western blot analysis. In addition, neuronal distribution was examined by NeuN immunohistochemistry. In the present study, the mean number of NeuN‑immunoreactive neurons was marginally decreased in mouse and rat hippocampi during aging, although this change was not identified to be significantly different. However, CAT immunoreactivity was significantly increased in pyramidal and granule neurons in the adult mouse and rat hippocampi and was significantly decreased in the aged mouse and rat hippocampi compared with that in the young animals. CAT protein levels in the hippocampus were also lowest in the aged mouse and rat hippocampus. These results indicate that CAT expression is significantly decreased in the hippocampi of aged animals and decreased CAT expression may be closely associated with aging.
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July-2016
Volume 14 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ahn JH, Chen BH, Shin BN, Lee TK, Cho JH, Kim IH, Park JH, Lee JC, Tae HJ, Lee CH, Lee CH, et al: Comparison of catalase immunoreactivity in the hippocampus between young, adult and aged mice and rats. Mol Med Rep 14: 851-856, 2016
APA
Ahn, J.H., Chen, B.H., Shin, B., Lee, T., Cho, J.H., Kim, I.H. ... Hong, S. (2016). Comparison of catalase immunoreactivity in the hippocampus between young, adult and aged mice and rats. Molecular Medicine Reports, 14, 851-856. https://doi.org/10.3892/mmr.2016.5300
MLA
Ahn, J. H., Chen, B. H., Shin, B., Lee, T., Cho, J. H., Kim, I. H., Park, J. H., Lee, J., Tae, H., Lee, C., Won, M., Lee, Y. L., Choi, S. Y., Hong, S."Comparison of catalase immunoreactivity in the hippocampus between young, adult and aged mice and rats". Molecular Medicine Reports 14.1 (2016): 851-856.
Chicago
Ahn, J. H., Chen, B. H., Shin, B., Lee, T., Cho, J. H., Kim, I. H., Park, J. H., Lee, J., Tae, H., Lee, C., Won, M., Lee, Y. L., Choi, S. Y., Hong, S."Comparison of catalase immunoreactivity in the hippocampus between young, adult and aged mice and rats". Molecular Medicine Reports 14, no. 1 (2016): 851-856. https://doi.org/10.3892/mmr.2016.5300