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TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways

  • Authors:
    • Zi‑Yuan Lu
    • Wan‑Cheng Chen
    • Yong‑Hua Li
    • Li Li
    • Hang Zhang
    • Yan Pang
    • Zhi‑Fang Xiao
    • Hao‑Wen Xiao
    • Yang Xiao
  • View Affiliations / Copyright

    Affiliations: First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou, Guangdong 510010, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 643-648
    |
    Published online on: May 19, 2016
       https://doi.org/10.3892/mmr.2016.5314
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Abstract

The migration of circulating mesenchymal stem cells (MSCs) to injured tissue is an important step in tissue regeneration and requires adhesion to the microvascular endothelium. The current study investigated the underlying mechanism of MSC adhesion to endothelial cells during inflammation. In in vitro MSC culture, tumor necrosis factor‑α (TNF‑α) increased the level of vascular cell adhesion molecule‑1 (VCAM‑1) expression in a dose‑dependent manner. The nuclear factor-κB (NF-κB), extracellular signal‑regulated kinase (ERK) and c‑Jun N‑terminal kinase (JNK) signaling pathway inhibitors, pyrrolidine dithiocarbamate (PDTC), U0126 and SP600125, respectively, suppressed VCAM‑1 expression induced by TNF‑α at the mRNA and protein levels (P<0.05). TNF‑α augmented the activation of NF‑κB, ERK and JNK, and promoted MSC adhesion to human umbilical vein endothelial cells; however, the inhibitors of NF‑κB, ERK and JNK did not affect this process in these cells. The results of the current study indicate that adhesion of circulating MSCs to the endothelium is regulated by TNF-α-induced VCAM-1 expression, which is potentially mediated by the NF‑κB, ERK and JNK signaling pathways.
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1 

Friedenstein AJ, Chailakhyan RK, Latsinik NV, Panasyuk AF and Keiliss-Borok IV: Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo. Transplantation. 17:331–340. 1974. View Article : Google Scholar : PubMed/NCBI

2 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Caplan AI: Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol. 213:341–347. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Nauta AJ and Fibbe WE: Immunomodulatory properties of mesenchymal stromal cells. Blood. 110:3499–3506. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Gao J, Dennis JE, Muzic RF, Lundberg M and Caplan AI: The dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion. Cells Tissues Organs. 169:12–20. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Devine SM, Cobbs C, Jennings M, Bartholomew A and Hoffman R: Mesenchymal stem cells distribute to a wide range of tissues following systemic infusion into nonhuman primates. Blood. 101:2999–3001. 2003. View Article : Google Scholar

7 

Fu X, Han B, Cai S, Lei Y, Sun T and Sheng Z: Migration of bone marrow-derived mesenchymal stem cells induced by tumor necrosis factor-alpha and its possible role in wound healing. Wound Repair Regen. 17:185–191. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Zhang A, Wang Y, Ye Z, Xie H, Zhou L and Zheng S: Mechanism of TNF-α-induced migration and hepatocyte growth factor production in human mesenchymal stem cells. J Cell Biochem. 111:469–475. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L and Sobiesiak M: Phenotypic characterization of distinct human bone marrow-derived MSC subsets. Ann N Y Acad Sci. 1176:124–134. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Teo GS, Ankrum JA, Martinelli R, Boetto SE, Simms K, Sciuto TE, Dvorak AM, Karp JM and Carman CV: Mesenchymal stem cells transmigrate between and directly through tumor necrosis factor-α-activated endothelial cells via both leukocyte-like and novel mechanisms. Stem Cells. 30:2472–2486. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Segers VF, Van Riet I, Andries LJ, Lemmens K, Demolder MJ, De Becker AJ, Kockx MM and De Keulenaer GW: Mesenchymal stem cell adhesion to cardiac microvascular endothelium: Activators and mechanisms. Am J Physiol Heart Circ Physiol. 290:H1370–H1377. 2006. View Article : Google Scholar

12 

Zhong X, Li X, Liu F, Tan H and Shang D: Omentin inhibits TNF-α-induced expression of adhesion molecules in endothelial cells via ERK/NF-kB pathway. Biochem Biophys Res Commun. 425:401–406. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Choi H, Nguyen HN and Lamb FS: Inhibition of endocytosis exacerbates TNF-α-induced endothelial dysfunction via enhanced JNK and p38 activation. Am J Physiol Heart Circ Physiol. 306:H1154–H1163. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Method Methods. 25:402–408. 2001. View Article : Google Scholar

15 

Xiao Y, Jiang ZJ, Pang Y, Li L, Gao Y, Xiao HW, Li YH, Zhang H and Liu Q: Efficacy and safety of mesenchymal stem cell treatment from related donors for patients with refractory aplastic anemia. Cytotherapy. 15:760–766. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Rodrigo SF, van Ramshorst J, Hoogslag GE, Boden H, Velders MA, Cannegieter SC, Roelofs H, Al Younis I, Dibbets-Schneider P, Fibbe WE, et al: Intramyocardial injection of autologous bone marrow-derived ex vivo expanded mesenchymal stem cells in acute myocardial infarction patients is feasible and safe up to 5 years of follow-up. J Cardiovasc Transl Res. 6:816–825. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Huleihel L, Levine M and Rojas M: The potential of cell-based therapy in lung diseases. Expert Opin Biol Ther. 13:1429–1440. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Undale AH, Westendorf JJ, Yaszemski MJ and Khosla S: Mesenchymal stem cells for bone repair and metabolic bone diseases. Mayo Clin Proc. 84:893–902. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Weng JY, Du X, Geng SX, Peng YW, Wang Z, Lu ZS, Wu SJ, Luo CW, Guo R, Ling W, et al: Mesenchymal stem cell as salvage treatment for refractory chronic GVHD. Bone Marrow Transplant. 45:1732–1740. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Croitoru-Lamoury J, Lamoury FM, Zaunders JJ, Veas LA and Brew BJ: Human mesenchymal stem cells constitutively express chemokines and chemokine receptors that can be upregulated by cytokines, IFN-beta and Copaxone. J Interferon Cytokine Res. 27:53–64. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Hu Y, Cheng P, Ma JC, Xue YX and Liu YH: Platelet-derived growth factor BB mediates the glioma-induced migration of bone marrow-derived mesenchymal stem cells by promoting the expression of vascular cell adhesion molecule-1 through the PI3K, P38 MAPK and NF-kB pathways. Oncol Rep. 30:2755–2764. 2013.

22 

Gao Z, Cheng P, Xue Y and Liu Y: Vascular endothelial growth factor participates in modulating the C6 glioma-induced migration of rat bone marrow-derived mesenchymal stem cells and upregulates their vascular cell adhesion molecule-1 expression. Exp Ther Med. 4:993–998. 2012.PubMed/NCBI

23 

Uchibori R, Tsukahara T, Mizuguchi H, Saga Y, Urabe M, Mizukami H, Kume A and Ozawa K: NF-kB activity regulates mesenchymal stem cell accumulation at tumor sites. Cancer Res. 73:364–372. 2013. View Article : Google Scholar

24 

Xiao Q, Wang SK, Tian H, Xin L, Zou ZG, Hu YL, Chang CM, Wang XY, Yin QS, Zhang XH and Wang LY: TNF-α increases bone marrow mesenchymal stem cell migration to ischemic tissues. Cell Biochem Biophys. 62:409–414. 2012. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lu ZY, Chen WC, Li YH, Li L, Zhang H, Pang Y, Xiao ZF, Xiao HW and Xiao Y: TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways. Mol Med Rep 14: 643-648, 2016.
APA
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y. ... Xiao, Y. (2016). TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways. Molecular Medicine Reports, 14, 643-648. https://doi.org/10.3892/mmr.2016.5314
MLA
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y., Xiao, Z., Xiao, H., Xiao, Y."TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways". Molecular Medicine Reports 14.1 (2016): 643-648.
Chicago
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y., Xiao, Z., Xiao, H., Xiao, Y."TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways". Molecular Medicine Reports 14, no. 1 (2016): 643-648. https://doi.org/10.3892/mmr.2016.5314
Copy and paste a formatted citation
x
Spandidos Publications style
Lu ZY, Chen WC, Li YH, Li L, Zhang H, Pang Y, Xiao ZF, Xiao HW and Xiao Y: TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways. Mol Med Rep 14: 643-648, 2016.
APA
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y. ... Xiao, Y. (2016). TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways. Molecular Medicine Reports, 14, 643-648. https://doi.org/10.3892/mmr.2016.5314
MLA
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y., Xiao, Z., Xiao, H., Xiao, Y."TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways". Molecular Medicine Reports 14.1 (2016): 643-648.
Chicago
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y., Xiao, Z., Xiao, H., Xiao, Y."TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways". Molecular Medicine Reports 14, no. 1 (2016): 643-648. https://doi.org/10.3892/mmr.2016.5314
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