Open Access

TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways

  • Authors:
    • Zi‑Yuan Lu
    • Wan‑Cheng Chen
    • Yong‑Hua Li
    • Li Li
    • Hang Zhang
    • Yan Pang
    • Zhi‑Fang Xiao
    • Hao‑Wen Xiao
    • Yang Xiao
  • View Affiliations

  • Published online on: May 19, 2016     https://doi.org/10.3892/mmr.2016.5314
  • Pages: 643-648
  • Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The migration of circulating mesenchymal stem cells (MSCs) to injured tissue is an important step in tissue regeneration and requires adhesion to the microvascular endothelium. The current study investigated the underlying mechanism of MSC adhesion to endothelial cells during inflammation. In in vitro MSC culture, tumor necrosis factor‑α (TNF‑α) increased the level of vascular cell adhesion molecule‑1 (VCAM‑1) expression in a dose‑dependent manner. The nuclear factor-κB (NF-κB), extracellular signal‑regulated kinase (ERK) and c‑Jun N‑terminal kinase (JNK) signaling pathway inhibitors, pyrrolidine dithiocarbamate (PDTC), U0126 and SP600125, respectively, suppressed VCAM‑1 expression induced by TNF‑α at the mRNA and protein levels (P<0.05). TNF‑α augmented the activation of NF‑κB, ERK and JNK, and promoted MSC adhesion to human umbilical vein endothelial cells; however, the inhibitors of NF‑κB, ERK and JNK did not affect this process in these cells. The results of the current study indicate that adhesion of circulating MSCs to the endothelium is regulated by TNF-α-induced VCAM-1 expression, which is potentially mediated by the NF‑κB, ERK and JNK signaling pathways.
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July-2016
Volume 14 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lu ZY, Chen WC, Li YH, Li L, Zhang H, Pang Y, Xiao ZF, Xiao HW and Xiao Y: TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways. Mol Med Rep 14: 643-648, 2016
APA
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y. ... Xiao, Y. (2016). TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways. Molecular Medicine Reports, 14, 643-648. https://doi.org/10.3892/mmr.2016.5314
MLA
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y., Xiao, Z., Xiao, H., Xiao, Y."TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways". Molecular Medicine Reports 14.1 (2016): 643-648.
Chicago
Lu, Z., Chen, W., Li, Y., Li, L., Zhang, H., Pang, Y., Xiao, Z., Xiao, H., Xiao, Y."TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways". Molecular Medicine Reports 14, no. 1 (2016): 643-648. https://doi.org/10.3892/mmr.2016.5314