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Article

1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway

  • Authors:
    • Lina Yang
    • Lan Wu
    • Shuyan Du
    • Ye Hu
    • Yi Fan
    • Jianfei Ma
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Geriatrics, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China, Central Laboratory, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 839-844
    |
    Published online on: May 23, 2016
       https://doi.org/10.3892/mmr.2016.5323
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Abstract

The regulation of cell proliferation, differentiation and immunomodulation are affected by 1,25(OH)2D3. However, its function during apoptosis and oxidative stress in human peritoneal mesothelial cells (HPMCs) remains unknown. The aim of the present study was to investigate whether the regulation of apoptosis and oxidative stress have therapeutic relevance in peritoneal dialysis (PD) therapy. The present study investigated the effects of 1,25(OH)2D3 on high glucose (HG)-induced apoptosis and reactive oxygen species (ROS) production in HPMCs, and examined the underlying molecular mechanisms. Flow cytometry and western blotting were performed to detect cell apoptosis, 2,7-dichlorofluorescein diacetate was used to measure reactive oxygen species production and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to measure cell viability. The results of the present study demonstrated that exposure to HG increased apoptosis and ROS production in HPMCs, whereas pretreatment with 1,25(OH)2D3 significantly inhibited HG‑induced apoptosis and ROS production. Further analysis revealed that 1,25(OH)2D3 facilitated cell survival via the MAPK/P38 pathway. The results of the present study indicate that 1,25(OH)2D3 inhibits apoptosis and ROS production in HG‑induced HPMCs via inhibition of the MAPK/P38 pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Wu L, Du S, Hu Y, Fan Y and Ma J: 1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway. Mol Med Rep 14: 839-844, 2016.
APA
Yang, L., Wu, L., Du, S., Hu, Y., Fan, Y., & Ma, J. (2016). 1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway. Molecular Medicine Reports, 14, 839-844. https://doi.org/10.3892/mmr.2016.5323
MLA
Yang, L., Wu, L., Du, S., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway". Molecular Medicine Reports 14.1 (2016): 839-844.
Chicago
Yang, L., Wu, L., Du, S., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway". Molecular Medicine Reports 14, no. 1 (2016): 839-844. https://doi.org/10.3892/mmr.2016.5323
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Wu L, Du S, Hu Y, Fan Y and Ma J: 1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway. Mol Med Rep 14: 839-844, 2016.
APA
Yang, L., Wu, L., Du, S., Hu, Y., Fan, Y., & Ma, J. (2016). 1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway. Molecular Medicine Reports, 14, 839-844. https://doi.org/10.3892/mmr.2016.5323
MLA
Yang, L., Wu, L., Du, S., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway". Molecular Medicine Reports 14.1 (2016): 839-844.
Chicago
Yang, L., Wu, L., Du, S., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway". Molecular Medicine Reports 14, no. 1 (2016): 839-844. https://doi.org/10.3892/mmr.2016.5323
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