1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway

Yang,Lina; Wu,Lan; Du,Shuyan; Hu,Ye; Fan,Yi; Ma,Jianfei

doi:10.3892/mmr.2016.5323

1,25(OH)2D3 inhibits high glucose-induced apoptosis and ROS production in human peritoneal mesothelial cells via the MAPK/P38 pathway

Authors:
- Lina Yang
- Lan Wu
- Shuyan Du
- Ye Hu
- Yi Fan
- Jianfei Ma
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Affiliations: Department of Nephrology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Geriatrics, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China, Central Laboratory, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: May 23, 2016 https://doi.org/10.3892/mmr.2016.5323
Pages: 839-844

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Abstract

The regulation of cell proliferation, differentiation and immunomodulation are affected by 1,25(OH)2D3. However, its function during apoptosis and oxidative stress in human peritoneal mesothelial cells (HPMCs) remains unknown. The aim of the present study was to investigate whether the regulation of apoptosis and oxidative stress have therapeutic relevance in peritoneal dialysis (PD) therapy. The present study investigated the effects of 1,25(OH)2D3 on high glucose (HG)-induced apoptosis and reactive oxygen species (ROS) production in HPMCs, and examined the underlying molecular mechanisms. Flow cytometry and western blotting were performed to detect cell apoptosis, 2,7-dichlorofluorescein diacetate was used to measure reactive oxygen species production and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to measure cell viability. The results of the present study demonstrated that exposure to HG increased apoptosis and ROS production in HPMCs, whereas pretreatment with 1,25(OH)2D3 significantly inhibited HG‑induced apoptosis and ROS production. Further analysis revealed that 1,25(OH)2D3 facilitated cell survival via the MAPK/P38 pathway. The results of the present study indicate that 1,25(OH)2D3 inhibits apoptosis and ROS production in HG‑induced HPMCs via inhibition of the MAPK/P38 pathway.

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1	Mortier S, De Vriese AS and Lameire N: Recent concepts in the molecular biology of the peritoneal membrane - implications for more biocompatible dialysis solutions. Blood Purif. 21:14–23. 2003. View Article : Google Scholar : PubMed/NCBI
2	Ahmad M, Shah H, Pliakogiannis T and Oreopoulos DG: Prevention of membrane damage in patient on peritoneal dialysis with new peritoneal dialysis solutions. Int Urol Nephrol. 39:299–312. 2007. View Article : Google Scholar
3	Ghibelli L and Diederich M: Multistep and multitask Bax activation. Mitochondrion. 10:604–613. 2010. View Article : Google Scholar : PubMed/NCBI
4	Ortiz A: Nephrology forum: Apoptotic regulatory proteins in renal injury. Kidney Int. 58:467–485. 2000. View Article : Google Scholar : PubMed/NCBI
5	Zhang X, Liang D, Guo B, Yang L, Wang L and Ma J: Zinc inhibits high glucose-induced apoptosis in peritoneal mesothelial cells. Biol Trace Elem Res. 150:424–432. 2012. View Article : Google Scholar : PubMed/NCBI
6	Zheng ZH, Ye RG, Bergström J and Lindholm B: Effect of dialysate composition on the apoptosis and proliferation of human peritoneal mesothelial cells and protein expression of Fas and c-Myc. Adv Perit Dial. 16:31–35. 2000.PubMed/NCBI
7	Kaifu K, Kiyomoto H, Hitomi H, Matsubara K, Hara T, Moriwaki K, Ihara G, Fujita Y, Sugasawa N, Nagata D, et al: Insulin attenuates apoptosis induced by high glucose via the PI3-kinase/Akt pathway in rat peritoneal mesothelial cells. Nephrol Dial Transplant. 24:809–815. 2009. View Article : Google Scholar
8	Gocek E, Kiełbiński M, Wyłób P, Kutner A and Marcinkowska E: Side-chain modified vitamin D analogs induce rapid accumulation of VDR in the cell nuclei proportionately to their differentiation-inducing potential. Steroids. 73:1359–1366. 2008. View Article : Google Scholar : PubMed/NCBI
9	Yang L, Wang J, Fan Y, Chen S, Wang L and Ma J: Effect of 1,25(OH)₂D₃ on rat peritoneal mesothelial cells treated with high glucose plus lipopolysaccharide. Cell Immunol. 271:173–179. 2011. View Article : Google Scholar
10	Jain SK and Micinski D: Vitamin D upregulates glutamate cysteine ligase and glutathione reductase, and GSH formation, and decreases ROS and MCP-1 and IL-8 secretion in high-glucose exposed U937 monocytes. Biochem Biophys Res Commun. 437:7–11. 2013. View Article : Google Scholar : PubMed/NCBI
11	Chiu CT and Chuang DM: Molecular actions and therapeutic potential of lithium in preclinical and clinical studies of CNS disorders. Pharmacol Ther. 128:281–304. 2010. View Article : Google Scholar : PubMed/NCBI
12	Kim YH, Rane A, Lussier S and Andersen JK: Lithium protects against oxidative stress-mediated cell death in α-synuclein-overexpressing in vitro and in vivo models of Parkinson's disease. J Neurosci Res. 89:1666–1675. 2011. View Article : Google Scholar : PubMed/NCBI
13	McCubrey JA, Lahair MM and Franklin RA: Reactive oxygen species-induced activation of the MAP kinase signaling pathways. Antioxid Redox Signal. 8:1775–1789. 2006. View Article : Google Scholar : PubMed/NCBI
14	Kholodenko BN and Birtwistle MR: Four-dimensional dynamics of MAPK information processing systems. Wiley Interdiscip Rev Syst Biol Med. 1:28–44. 2009. View Article : Google Scholar
15	Jörres A and Witowski J: PD membrane: Biological responses to different PD fluids. Contrib Nephrol. 150:48–53. 2006. View Article : Google Scholar : PubMed/NCBI
16	Zou MS, Yu J, Nie GM, He WS, Luo LM and Xu HT: 1, 25-dihydroxyvitamin D3 decreases adriamycin-induced podocyte apoptosis and loss. Int J Med Sci. 7:290–299. 2010. View Article : Google Scholar : PubMed/NCBI
17	Greene DA, Stevens MJ, Obrosova I and Feldman EL: Glucose-induced oxidative stress and programmed cell death in diabetic neuropathy. Eur J Pharmacol. 375:217–223. 1999. View Article : Google Scholar : PubMed/NCBI
18	Lelkes E, Unsworth BR and Lelkes PI: Reactive oxygen species, apoptosis and altered NGF-induced signaling in PC12 pheochromocytoma cells cultured in elevated glucose: An in vitro cellular model for diabetic neuropathy. Neurotox Res. 3:189–203. 2001. View Article : Google Scholar
19	Lan N, Luo G, Yang X, Cheng Y, Zhang Y, Wang X, Wang X, Xie T, Li G, Liu Z and Zhong N: 25-Hydroxyvitamin d3-deficiency enhances oxidative stress and corticosteroid resistance in severe asthma exacerbation. PLoS One. 9:e1115992014. View Article : Google Scholar : PubMed/NCBI
20	Rehman S, Chandel N, Salhan D, Rai P, Sharma B, Singh T, Husain M, Malhotra A and Singhal PC: Ethanol and vitamin D receptor in T cell apoptosis. J Neuroimmune Pharmacol. 8:251–261. 2013. View Article : Google Scholar :
21	Raman M, Chen W and Cobb MH: Differential regulation and properties of MAPKs. Oncogene. 26:3100–3112. 2007. View Article : Google Scholar : PubMed/NCBI
22	Liu B, Jian Z, Li Q, Li K, Wang Z, Liu L, Tang L, Yi X, Wang H, Li C and Gao T: Baicalein protects human melanocytes from H₂O₂-induced apoptosis via inhibiting mitochondria-dependent caspase activation and the p38 MAPK pathway. Free Radic Biol Med. 53:183–193. 2012. View Article : Google Scholar : PubMed/NCBI
23	Lee HJ, Noh YH, Lee DY, Kim YS, Kim KY, Chung YH, Lee WB and Kim SS: Baicalein attenuates 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells. Eur J Cell Biol. 84:897–905. 2005. View Article : Google Scholar : PubMed/NCBI
24	Chen YC, Chow JM, Lin CW, Wu CY and Shen SC: Baicalein inhibition of oxidative-stress-induced apoptosis via modulation of ERKs activation and induction of HO-1 gene expression in rat glioma cells C6. Toxicol Appl Pharmacol. 216:263–273. 2006. View Article : Google Scholar : PubMed/NCBI
25	Lin HY, Shen SC, Lin CW, Yang LY and Chen YC: Baicalein inhibition of hydrogen peroxide-induced apoptosis via ROS-dependent heme oxygenase 1 gene expression. Biochim Biophys Acta. 1773:1073–1086. 2007. View Article : Google Scholar : PubMed/NCBI
26	Nagai H, Noguchi T, Takeda K and Ichijo H: Pathophysiological roles of ASK1-MAP kinase signaling pathways. J Biochem Mol Biol. 40:1–6. 2007. View Article : Google Scholar : PubMed/NCBI
27	Chen HM, Hsu JH, Liou SF, Chen TJ, Chen LY, Chiu CC and Yeh JL: Baicalein, an active component of Scutellaria baicalensis Georgi, prevents lysophosphatidylcholine-induced cardiac injury by reducing reactive oxygen species production, calcium overload and apoptosis via MAPK pathways. BMC Complement Altern Med. 14:2332014. View Article : Google Scholar : PubMed/NCBI