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Article Open Access

Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats

  • Authors:
    • Haipeng Sun
    • Jinming Wang
    • Feilong Deng
    • Yun Liu
    • Xiumei Zhuang
    • Jiayun Xu
    • Long Li
  • View Affiliations / Copyright

    Affiliations: Department of Oral Implantology, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat‑Sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Oral Implantology, Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat‑Sen University, Guangzhou, Guangdong 510055, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 737-745
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    Published online on: May 24, 2016
       https://doi.org/10.3892/mmr.2016.5339
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Abstract

There has been considerable focus in investigations on the delivery systems and clinical applications of bone morphogenetic protein‑2 (BMP‑2) for novel bone formation. However, current delivery systems require high levels of BMP‑2 to exert a biological function. There are several concerns in using of high levels of BMP‑2, including safety and the high cost of treatment. Therefore, the development of strategies to decrease the levels of BMP‑2 required in these delivery systems is required. In our previous studies, a controlled‑release system was developed, which used Traut's reagent and the cross‑linker, 4‑(N‑maleimi‑domethyl) cyclohexane‑1‑carboxylic acid 3‑sulfo‑N‑hydroxysuccinimide ester sodium salt (Sulfo‑SMCC), to chemically conjugate BMP‑2 directly on collagen discs. In the current study, retention efficiency and release kinetics of stromal cell‑derived factor‑1α (SDF‑1α) cross‑linked on collagen scaffolds were detected. In addition, the osteogenic activity of SDF‑1α and suboptimal doses of BMP‑2 cross‑linked on collagen discs following subcutaneous implantation in rats were evaluated. Independent two‑tailed t‑tests and one‑way analysis of variance were used for analysis. In the present study, the controlled release of SDF‑1α chemically conjugated on collagen scaffolds was demonstrated. By optimizing the concentrations of Traut's reagent and the Sulfo‑SMCC cross‑linker, a significantly higher level of SDF‑1α was covalently retained on the collagen scaffold, compared with that retained using a physical adsorption method. Mesenchymal stem cell homing indicated that the biological function of the SDF‑1α cross‑linked on the collagen scaffolds remained intact. In rats, co‑treatment with SDF‑1α and a suboptimal dose of BMP‑2 cross‑linked on collagen scaffolds using this chemically conjugated method induced higher levels of ectopic bone formation, compared with the physical adsorption method. No ectopic bone formation was observed following treatment with a suboptimal dose of BMP‑2 alone. Therefore, the co‑delivery of SDF‑1α and a suboptimal dose of BMP‑2 chemically conjugated on collagen scaffolds for the treatment of bone injuries reduced the level of BMP‑2 required, reducing the risks of side effects.
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View References

1 

Dimitriou R, Jones E, McGonagle D and Giannoudis PV: Bone regeneration: Current concepts and future directions. BMC Med. 9:662011. View Article : Google Scholar : PubMed/NCBI

2 

Schwartz CE, Martha JF, Kowalski P, Wang DA, Bode R, Li L and Kim DH: Prospective evaluation of chronic pain associated with posterior autologous iliac crest bone graft harvest and its effect on postoperative outcome. Health Qual Life Outcomes. 7:492009. View Article : Google Scholar : PubMed/NCBI

3 

Kim DH, Rhim R, Li L, Martha J, Swaim BH, Banco RJ, Jenis LG and Tromanhauser SG: Prospective study of iliac crest bone graft harvest site pain and morbidity. Spine J. 9:886–892. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Vo TN, Kasper FK and Mikos AG: Strategies for controlled delivery of growth factors and cells for bone regeneration. Adv Drug Deliv Rev. 64:1292–1309. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Schindeler A, McDonald MM, Bokko P and Little DG: Bone remodeling during fracture repair: The cellular picture. Semin Cell Dev Biol. 19:459–466. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Barnes GL, Kostenuik PJ, Gerstenfeld LC and Einhorn TA: Growth factor regulation of fracture repair. J Bone Miner Res. 14:1805–1815. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Nauth A, Ristevski B, Li R and Schemitsch EH: Growth factors and bone regeneration: How much bone can we expect? Injury. 42:574–579. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Giannoudis PV and Einhorn TA: Bone morphogenetic proteins in musculoskeletal medicine. Injury. 40(Suppl 3): S1–S3. 2009.

9 

Lieberman JR, Daluiski A and Einhorn TA: The role of growth factors in the repair of bone. Biology and clinical applications. J Bone Joint Surg Am. 84-A:1032–1044. 2002.PubMed/NCBI

10 

Seeherman H and Wozney JM: Delivery of bone morphogenetic proteins for orthopedic tissue regeneration. Cytokine Growth. Factor Rev. 16:329–345. 2005. View Article : Google Scholar

11 

Argintar E, Edwards S and Delahay J: Bone morphogenetic proteins in orthopaedic trauma surgery. Injury. 42:730–734. 2011. View Article : Google Scholar

12 

Kroese-Deutman HC, Ruhé PQ, Spauwen PH and Jansen JA: Bone inductive properties of rhBMP-2 loaded porous calcium phosphate cement implants inserted at an ectopic site in rabbits. Biomaterials. 26:1131–1138. 2005. View Article : Google Scholar

13 

Ishibe T, Goto T, Kodama T, Miyazaki T, Kobayashi S and Takahashi T: Bone formation on apatite-coated titanium with incorporated BMP-2/heparin in vivo. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 108:867–875. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Yang HS, La WG, Bhang SH, Lee TJ, Lee M and Kim BS: Apatite-coated collagen scaffold for bone morphogenetic protein-2 delivery. Tissue Eng Part A. 17:1–12. 2011. View Article : Google Scholar

15 

Fu TS, Chang YH, Wong CB, Wang IC, Tsai TT, Lai PL, Chen LH and Chen WJ: Mesenchymal stem cells expressing baculovirus-engineered BMP-2 and VEGF enhance postero-lateral spine fusion in a rabbit model. Spine J. 15:2036–2044. 2015. View Article : Google Scholar

16 

Huang RL, Chen G, Wang W, Herller T, Xie Y, Gu B and Li Q: Synergy between IL-6 and soluble IL-6 receptor enhances bone morphogenetic protein-2/absorbable collagen sponge-induced bone regeneration via regulation of BMPRIA distribution and degradation. Biomaterials. 67:308–322. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Hwang HD, Lee JT, Koh JT, Jung HM, Lee HJ and Kwon TG: Sequential treatment with SDF-1 and BMP-2 potentiates bone formation in calvarial defects. Tissue Eng Part A. 21:2125–2135. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Zlotnik A and Yoshie O: Chemokines: A new classification system and their role in immunity. Immunity. 12:121–127. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Bleul CC, Farzan M, Choe H, Parolin C, Cla rk-Lewis I, Sodroski J and Springer TA: The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 382:829–833. 1996. View Article : Google Scholar : PubMed/NCBI

20 

Feng Y, Broder CC, Kennedy PE and Berger EA: HIV-1 entry cofactor: Functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 272:872–877. 1996. View Article : Google Scholar : PubMed/NCBI

21 

Iannone M, Ventre M, Pagano G, Giannoni P, Quarto R and Netti PA: Defining an optimal stromal derived factor-1 presentation for effective recruitment of mesenchymal stem cells in 3D. Biotechnol Bioeng. 111:2303–2316. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Naderi-Meshkin H, Bahrami AR, Bidkhori HR, Mirahmadi M and Ahmadiankia N: Strategies to improve homing of mesenchymal stem cells for greater efficacy in stem cell therapy. Cell Biol Int. 39:23–34. 2015. View Article : Google Scholar

23 

Granero-Moltó F, Weis JA, Miga MI, Landis B, Myers TJ, O'Rear L, Longobardi L, Jansen ED, Mortlock DP and Spagnoli A: Regenerative effects of transplanted mesenchymal stem cells in fracture healing. Stem Cells. 27:1887–1898. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Otsuru S, Tamai K, Yamazaki T, Yoshikawa H and Kaneda Y: Circulating bone marrow-derived osteoblast progenitor cells are recruited to the bone-forming site by the CXCR4/stromal cell-derived factor-1 pathway. Stem Cells. 26:223–234. 2008. View Article : Google Scholar

25 

Hosogane N, Huang Z, Rawlins BA, Liu X, Boachie-Adjei O, Boskey AL and Zhu W: Stromal derived factor-1 regulates bone morphogenetic protein 2-induced osteogenic differentiation of primary mesenchymal stem cells. Int J Biochem Cell Biol. 42:1132–1141. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Zhu W, Boachie-Adjei O, Rawlins BA, Frenkel B, Boskey AL, Ivashkiv LB and Blobel CP: A novel regulatory role for stromal-derived factor-1 signaling in bone morphogenic protein-2 osteogenic differentiation of mesenchymal C2C12 cells. J Biol Chem. 282:18676–18685. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Herberg S, Susin C, Pelaez M, Howie RN, Moreno de Freitas R, Lee J, Cray JJ Jr, Johnson MH, Elsalanty ME, Hamrick MW, et al: Low-dose bone morphogenetic protein-2/stromal cell-derived factor-1β cotherapy induces bone regeneration in critical-size rat calvarial defects. Tissue Eng Part A. 20:1444–1453. 2014. View Article : Google Scholar :

28 

Higashino K, Viggeswarapu M, Bargouti M, Liu H, Titus L and Boden SD: Stromal cell-derived factor-1 potentiates bone morphogenetic protein-2 induced bone formation. Tissue Eng Part A. 17:523–530. 2011. View Article : Google Scholar

29 

Ratanavaraporn J, Furuya H, Kohara H and Ta bat a Y: Synergistic effects of the dual release of stromal cell-derived factor-1 and bone morphogenetic protein-2 from hydrogels on bone regeneration. Biomaterials. 32:2797–2811. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Zhang Q, He QF, Zhang TH, Yu XL, Liu Q and Deng FL: Improvement in the delivery system of bone morphogenetic protein-2: A new approach to promote bone formation. Biomed Mater. 7:450022012. View Article : Google Scholar

31 

He Q, Zhao Y, Chen B, Xiao Z, Zhang J, Chen L, Chen W, Deng F and Dai J: Improved cellularization and angiogenesis using collagen scaffolds chemically conjugated with vascular endothelial growth factor. Acta Biomater. 7:1084–1093. 2011. View Article : Google Scholar

32 

Chen L, He Z, Chen B, Zhao Y, Sun W, Xiao Z, Zhang J, Yang M, Gao Z and Dai J: Direct chemical cross-linking of platelet-derived growth factor-BB to the demineralized bone matrix improves cellularization and vascularization. Biomacromolecules. 10:3193–3198. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Chen B, Lin H, Wang J, Zhao Y, Wang B, Zhao W, Sun W and Dai J: Homogeneous osteogenesis and bone regeneration by demineralized bone matrix loading with collagen-targeting bone morphogenetic protein-2. Biomaterials. 28:1027–1035. 2007. View Article : Google Scholar

34 

Kolácná L, Bakesová J, Varga F, Kostáková E, Plánka L, Necas A, Lukás D, Amler E and Pelouch V: Biochemical and biophysical aspects of collagen nanostructure in the extracellular matrix. Physiol Res. 56(Suppl 1): S51–S60. 2007.PubMed/NCBI

35 

Venugopal J and Ramakrishna S: Biocompatible nanofiber matrices for the engineering of a dermal substitute for skin regeneration. Tissue Eng. 11:847–854. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Li X, Feng Q, Liu X, Dong W and Cui F: Collagen-based implants reinforced by chitin fibres in a goat shank bone defect model. Biomaterials. 27:1917–1923. 2006. View Article : Google Scholar

37 

Boccafoschi F, Habermehl J, Vesentini S and Mantovani D: Biological performances of collagen-based scaffolds for vascular tissue engineering. Biomaterials. 26:7410–7417. 2005. View Article : Google Scholar : PubMed/NCBI

38 

Park SN, Kim JK and Suh H: Evaluation of antibiotic-loaded collagen-hyaluronic acid matrix as a skin substitute. Biomaterials. 25:3689–3698. 2004. View Article : Google Scholar : PubMed/NCBI

39 

Jue R, Lambert JM, Pierce LR and Traut RR: Addition of sulfhydryl groups to Escherichia coli ribosomes by protein modification with 2-iminothiolane (methyl 4-mercaptobutyrimidate). Biochemistry. 17:5399–5406. 1978. View Article : Google Scholar : PubMed/NCBI

40 

Geiger M, Li RH and Friess W: Collagen sponges for bone regeneration with rhBMP-2. Adv Drug Deliv Rev. 55:1613–1629. 2003. View Article : Google Scholar : PubMed/NCBI

41 

McQuibban GA, Butler GS, Gong JH, Bendall L, Power C, Clark-Lewis I and Overall CM: Matrix metalloproteinase activity inactivates the CXC chemokine stromal cell-derived factor-1. J Biol Chem. 276:43503–43508. 2001. View Article : Google Scholar : PubMed/NCBI

42 

Gomes ME, Sikavitsas VI, Behravesh E, Reis RL and Mikos AG: Effect of flow perfusion on the osteogenic differentiation of bone marrow stromal cells cultured on starch-based three-dimensional scaffolds. J Biomed Mater Res A. 67:87–95. 2003. View Article : Google Scholar : PubMed/NCBI

43 

Richardson TP, Peters MC, Ennett AB and Mooney DJ: Polymeric system for dual growth factor delivery. Nat Biotechnol. 19:1029–1034. 2001. View Article : Google Scholar : PubMed/NCBI

44 

Kanczler JM, Ginty PJ, White L, Clarke NM, Howdle SM, Shakesheff KM and Oreffo RO: The effect of the delivery of vascular endothelial growth factor and bone morphogenic protein-2 to osteoprogenitor cell populations on bone formation. Biomaterials. 31:1242–1250. 2010. View Article : Google Scholar

45 

Chen FM, Chen R, Wang XJ, Sun HH and Wu ZF: In vitro cellular responses to scaffolds containing two microencapulated growth factors. Biomaterials. 30:5215–5224. 2009. View Article : Google Scholar : PubMed/NCBI

46 

Patel ZS, Young S, Tabata Y, Jansen JA, Wong ME and Mikos AG: Dual delivery of an angiogenic and an osteogenic growth factor for bone regeneration in a critical size defect model. Bone. 43:931–940. 2008. View Article : Google Scholar : PubMed/NCBI

47 

Simmons CA, Alsberg E, Hsiong S, Kim WJ and Mooney DJ: Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells. Bone. 35:562–569. 2004. View Article : Google Scholar : PubMed/NCBI

48 

Herberg S, Kondrikova G, Periyasamy-Thandavan S, Howie RN, Elsalanty ME, Weiss L, Campbell P, Hill WD and Cray JJ: Inkjet-based biopatterning of SDF-1β augments BMP-2-induced repair of critical size calvarial bone defects in mice. Bone. 67:95–103. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun H, Wang J, Deng F, Liu Y, Zhuang X, Xu J and Li L: Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats. Mol Med Rep 14: 737-745, 2016.
APA
Sun, H., Wang, J., Deng, F., Liu, Y., Zhuang, X., Xu, J., & Li, L. (2016). Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats. Molecular Medicine Reports, 14, 737-745. https://doi.org/10.3892/mmr.2016.5339
MLA
Sun, H., Wang, J., Deng, F., Liu, Y., Zhuang, X., Xu, J., Li, L."Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats". Molecular Medicine Reports 14.1 (2016): 737-745.
Chicago
Sun, H., Wang, J., Deng, F., Liu, Y., Zhuang, X., Xu, J., Li, L."Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats". Molecular Medicine Reports 14, no. 1 (2016): 737-745. https://doi.org/10.3892/mmr.2016.5339
Copy and paste a formatted citation
x
Spandidos Publications style
Sun H, Wang J, Deng F, Liu Y, Zhuang X, Xu J and Li L: Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats. Mol Med Rep 14: 737-745, 2016.
APA
Sun, H., Wang, J., Deng, F., Liu, Y., Zhuang, X., Xu, J., & Li, L. (2016). Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats. Molecular Medicine Reports, 14, 737-745. https://doi.org/10.3892/mmr.2016.5339
MLA
Sun, H., Wang, J., Deng, F., Liu, Y., Zhuang, X., Xu, J., Li, L."Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats". Molecular Medicine Reports 14.1 (2016): 737-745.
Chicago
Sun, H., Wang, J., Deng, F., Liu, Y., Zhuang, X., Xu, J., Li, L."Co‑delivery and controlled release of stromal cell‑derived factor‑1α chemically conjugated on collagen scaffolds enhances bone morphogenetic protein‑2‑driven osteogenesis in rats". Molecular Medicine Reports 14, no. 1 (2016): 737-745. https://doi.org/10.3892/mmr.2016.5339
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