Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2016 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2016 Volume 14 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells

  • Authors:
    • Jin Shang
    • Honggang Wang
    • Xin Fan
    • Lei Shangguan
    • Huan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China
  • Pages: 1389-1396
    |
    Published online on: June 2, 2016
       https://doi.org/10.3892/mmr.2016.5359
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Low back pain is a prevalent disease, which leads to suffering and disabilities in a vast number of individuals. Degenerative disc diseases are usually the underlying causes of low back pain. However, the pathogenesis of degenerative disc diseases is highly complex and difficult to determine. Current therapies for degenerative disc diseases are various. In particular, cell-based therapies have proven to be effective and promising. Our research group has previously isolated and identified the cartilage endplate‑derived stem cells. In addition, alternative splicing is a sophisticated regulatory mechanism, which greatly increases cellular complexity and phenotypic diversity of eukaryotic organisms. The present study continued to investigate alternative splicing events in osteogenic differentiation of cartilage endplate‑derived stem cells. An Affymetrix Human Transcriptome Array 2.0 was used to detect splicing changes between the control and differentiated samples. Additionally, molecular function and pathway analysis were also performed. Following rigorous bioinformatics analysis of the data, 3,802 alternatively spliced genes were identified, and 10 of these were selected for validation by reverse transcription‑polymerase chain reaction. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway analysis also revealed numerous enriched GO terms and signaling pathways. To the best of our knowledge, the present study is the first to investigate alternative splicing mechanisms in osteogenic differentiation of stem cells on a genome‑wide scale. The illumination of molecular mechanisms of stem cell osteogenic differentiation may assist the development novel bioengineered methods to treat degenerative disc diseases.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Andersson GB: Epidemiological features of chronic low-back pain. Lancet. 354:581–585. 1999. View Article : Google Scholar : PubMed/NCBI

2 

Walker BF: The prevalence of low back pain: A systematic review of the literature from 1966 to 1998. J Spinal Disord. 13:205–217. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Freemont AJ: The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain. Rheumatology (Oxford). 48:5–10. 2009. View Article : Google Scholar

4 

Stokes IA and Iatridis JC: Mechanical conditions that accelerate intervertebral disc degeneration: Overload versus immobilization. Spine (Phila Pa 1976). 29:2724–2732. 2004. View Article : Google Scholar

5 

Urban JP, Smith S and Fairbank JC: Nutrition of the intervertebral disc. Spine (Phila Pa 1976). 29:2700–2709. 2004. View Article : Google Scholar

6 

Battié MC and Videman T: Lumbar disc degeneration: Epidemiology and genetics. J Bone Joint Surg Am. 88(Suppl 2): S3–S9. 2006. View Article : Google Scholar

7 

Zhao CQ, Wang LM, Jiang LS and Dai LY: The cell biology of intervertebral disc aging and degeneration. Ageing Res Rev. 6:247–261. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Smith LJ, Nerurkar NL, Choi KS, Harfe BD and Elliott DM: Degeneration and regeneration of the intervertebral disc: Lessons from development. Dis Model Mech. 4:31–41. 2011. View Article : Google Scholar :

9 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Bajada S, Mazakova I, Richardson JB and Ashammakhi N: Updates on stem cells and their applications in regenerative medicine. J Tissue Eng Regen Med. 2:169–183. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Risbud MV, Guttapalli A, Tsai TT, Lee JY, Danielson KG, Vaccaro AR, Albert TJ, Gazit Z, Gazit D and Shapiro IM: Evidence for skeletal progenitor cells in the degenerate human intervertebral disc. Spine (Phila Pa 1976). 32:2537–2544. 2007. View Article : Google Scholar

12 

Sakaguchi Y, Sekiya I, Yagishita K and Muneta T: Comparison of human stem cells derived from various mesenchymal tissues: Superiority of synovium as a cell source. Arthritis Rheum. 52:2521–2529. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Liu LT, Huang B, Li CQ, Zhuang Y, Wang J and Zhou Y: Characteristics of stem cells derived from the degenerated human intervertebral disc cartilage endplate. PLoS One. 6:e262852011. View Article : Google Scholar : PubMed/NCBI

14 

Blanco JF, Graciani IF, Sanchez-Guijo FM, Muntión S, Hernandez-Campo P, Santamaria C, Carrancio S, Barbado MV, Cruz G, Gutierrez-Cosío S, et al: Isolation and characterization of mesenchymal stromal cells from human degenerated nucleus pulposus: Comparison with bone marrow mesenchymal stromal cells from the same subjects. Spine (Phila Pa 1976). 35:2259–2265. 2010. View Article : Google Scholar

15 

Boyd LM and Carter AJ: Injectable biomaterials and vertebral endplate treatment for repair and regeneration of the intervertebral disc. Eur Spine J. 15(Suppl 3): S414–S421. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Rajasekaran S, Venkatadass K, Naresh Babu J, Ganesh K and Shetty AP: Pharmacological enhancement of disc diffusion and differentiation of healthy, ageing and degenerated discs: Results from in-vivo serial post-contrast MRI studies in 365 human lumbar discs. Eur Spine J. 17:626–643. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Peng B, Hou S, Shi Q and Jia L: The relationship between cartilage end-plate calcification and disc degeneration: An experimental study. Chin Med J (Engl). 114:308–312. 2001.

18 

Roberts S, Urban JP, Evans H and Eisenstein SM: Transport properties of the human cartilage endplate in relation to its composition and calcification. Spine (Phila Pa 1976). 21:415–420. 1996. View Article : Google Scholar

19 

Antoniou J, Goudsouzian NM, Heathfield TF, Winterbottom N, Steffen T, Poole AR, Aebi M and Alini M: The human lumbar endplate. Evidence of changes in biosynthesis and denaturation of the extracellular matrix with growth, maturation, aging, and degeneration. Spine (Phila Pa 1976). 21:1153–1161. 1996. View Article : Google Scholar

20 

Benneker LM, Heini PF, Alini M, Anderson SE and Ito K: 2004 Young Investigator Award Winner: Vertebral endplate marrow contact channel occlusions and intervertebral disc degeneration. Spine (Phila Pa 1976). 30:167–173. 2005. View Article : Google Scholar

21 

Black DL: Mechanisms of alternative pre-messenger RNA splicing. Annu Rev Biochem. 72:291–336. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Blencowe BJ: Alternative splicing: New insights from global analyses. Cell. 126:37–47. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Pan Q, Shai O, Lee LJ, Frey BJ and Blencowe BJ: Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing. Nat Genet. 40:1413–1415. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Wang ET, Sandberg R, Luo S, Khrebtukova I, Zhang L, Mayr C, Kingsmore SF, Schroth GP and Burge CB: Alternative isoform regulation in human tissue transcriptomes. Nature. 456:470–476. 2008. View Article : Google Scholar : PubMed/NCBI

25 

David CJ and Manley JL: The search for alternative splicing regulators: New approaches offer a path to a splicing code. Genes Dev. 22:279–285. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Lukong KE, Chang KW, Khandjian EW and Richard S: RNA-binding proteins in human genetic disease. Trends Genet. 24:416–425. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Martinez-Contreras R, Cloutier P, Shkreta L, Fisette JF, Revil T and Chabot B: hnRNP proteins and splicing control. Adv Exp Med Biol. 623:123–147. 2007. View Article : Google Scholar

28 

Ng B, Yang F, Huston DP, Yan Y, Yang Y, Xiong Z, Peterson LE, Wang H and Yang XF: Increased noncanonical splicing of auto-antigen transcripts provides the structural basis for expression of untolerized epitopes. J Allergy Clin Immunol. 114:1463–1470. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Kazantseva J, Kivil A, Tints K, Kazantseva A, Neuman T and Palm K: Alternative splicing targeting the hTAF4-TAFH domain of TAF4 represses proliferation and accelerates chondrogenic differentiation of human mesenchymal stem cells. PLoS One. 8:e747992013. View Article : Google Scholar : PubMed/NCBI

30 

McAlinden A, Shim KH, Wirthlin L, Ravindran S and Hering TM: Quantification of type II procollagen splice forms using alternative transcript-qPCR (AT-qPCR). Matrix Biol. 31:412–420. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Longo A, Librizzi M, Naselli F, Caradonna F, Tobiasch E and Luparello C: PTHrP in differentiating human mesenchymal stem cells: Transcript isoform expression, promoter methylation, and protein accumulation. Biochimie. 95:1888–1896. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Thornemo M, Tallheden T, Sjogren Jansson E, Larsson A, Lovstedt K, Nannmark U, Brittberg M and Lindahl A: Clonal populations of chondrocytes with progenitor properties identified within human articular cartilage. Cells Tissues Organs. 180:141–150. 2005. View Article : Google Scholar : PubMed/NCBI

33 

Purdom E, Simpson KM, Robinson MD, Conboy JG, Lapuk AV and Speed TP: FIRMA: A method for detection of alternative splicing from exon array data. Bioinformatics. 24:1707–1714. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Srinivasan K, Shiue L, Hayes JD, Centers R, Fitzwater S, Loewen R, Edmondson LR, Bryant J, Smith M, Rommelfanger C, et al: Detection and measurement of alternative splicing using splicing-sensitive microarrays. Methods. 37:345–359. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Clark TA, Sugnet CW and Ares M Jr: Genomewide analysis of mRNA processing in yeast using splicing-specific microarrays. Science. 296:907–910. 2002. View Article : Google Scholar : PubMed/NCBI

36 

Ahmed TA and Hincke MT: Strategies for articular cartilage lesion repair and functional restoration. Tissue Eng Part B Rev. 16:305–329. 2010. View Article : Google Scholar

37 

Caldwell KL and Wang J: Cell-based articular cartilage repair: The link between development and regeneration. Osteoarthritis Cartilage. 23:351–362. 2015. View Article : Google Scholar :

38 

Veronesi F, Giavaresi G, Tschon M, Borsari V, Nicoli Aldini N and Fini M: Clinical use of bone marrow, bone marrow concentrate, and expanded bone marrow mesenchymal stem cells in cartilage disease. Stem Cells Dev. 22:181–192. 2013. View Article : Google Scholar

39 

Locke M, Feisst V and Dunbar PR: Concise review: Human adipose-derived stem cells: Separating promise from clinical need. Stem Cells. 29:404–411. 2011. View Article : Google Scholar : PubMed/NCBI

40 

Babadagli ME, Tezcan B, Yilmaz ST and Tufan AC: Matrilin-3 as a putative effector of C-type natriuretic peptide signaling during TGF-β induced chondrogenic differentiation of mesenchymal stem cells. Mol Biol Rep. 41:5549–5555. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Fernandes AM, Herlofsen SR, Karlsen TA, Kuchler AM, Fløisand Y and Brinchmann JE: Similar properties of chondrocytes from osteoarthritis joints and mesenchymal stem cells from healthy donors for tissue engineering of articular cartilage. PLoS One. 8:e629942013. View Article : Google Scholar : PubMed/NCBI

42 

Herlofsen SR, Bryne JC, Høiby T, Wang L, Issner R, Zhang X, Coyne MJ, Boyle P, Gu H, Meza-Zepeda LA, et al: Genome-wide map of quantified epigenetic changes during in vitro chon-drogenic differentiation of primary human mesenchymal stem cells. BMC Genomics. 14:1052013. View Article : Google Scholar

43 

Weber M, Sotoca AM, Kupfer P, Guthke R and van Zoelen EJ: Dynamic modelling of microRNA regulation during mesenchymal stem cell differentiation. BMC Syst Biol. 7:1242013. View Article : Google Scholar : PubMed/NCBI

44 

Kan Z, Rouchka EC, Gish WR and States DJ: Gene structure prediction and alternative splicing analysis using genomically aligned ESTs. Genome Res. 11:889–900. 2001. View Article : Google Scholar : PubMed/NCBI

45 

Petit V and Thiery JP: Focal adhesions: Structure and dynamics. Biol Cell. 92:477–494. 2000. View Article : Google Scholar

46 

Mitra SK, Hanson DA and Schlaepfer DD: Focal adhesion kinase: In command and control of cell motility. Nat Rev Mol Cell Biol. 6:56–68. 2005. View Article : Google Scholar : PubMed/NCBI

47 

Comoglio PM, Boccaccio C and Trusolino L: Interactions between growth factor receptors and adhesion molecules: Breaking the rules. Curr Opin Cell Biol. 15:565–571. 2003. View Article : Google Scholar : PubMed/NCBI

48 

Chen Z, Gibson TB, Robinson F, Silvestro L, Pearson G, Xu B, Wright A, Vanderbilt C and Cobb MH: MAP kinases. Chem Rev. 101:2449–2476. 2001. View Article : Google Scholar : PubMed/NCBI

49 

Yang SH, Sharrocks AD and Whitmarsh AJ: Transcriptional regulation by the MAP kinase signaling cascades. Gene. 320:3–21. 2003. View Article : Google Scholar : PubMed/NCBI

50 

Tanoue T and Nishida E: Docking interactions in the mitogen-activated protein kinase cascades. Pharmacol Ther. 93:193–202. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shang J, Wang H, Fan X, Shangguan L and Liu H: A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells. Mol Med Rep 14: 1389-1396, 2016.
APA
Shang, J., Wang, H., Fan, X., Shangguan, L., & Liu, H. (2016). A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells. Molecular Medicine Reports, 14, 1389-1396. https://doi.org/10.3892/mmr.2016.5359
MLA
Shang, J., Wang, H., Fan, X., Shangguan, L., Liu, H."A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells". Molecular Medicine Reports 14.2 (2016): 1389-1396.
Chicago
Shang, J., Wang, H., Fan, X., Shangguan, L., Liu, H."A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells". Molecular Medicine Reports 14, no. 2 (2016): 1389-1396. https://doi.org/10.3892/mmr.2016.5359
Copy and paste a formatted citation
x
Spandidos Publications style
Shang J, Wang H, Fan X, Shangguan L and Liu H: A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells. Mol Med Rep 14: 1389-1396, 2016.
APA
Shang, J., Wang, H., Fan, X., Shangguan, L., & Liu, H. (2016). A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells. Molecular Medicine Reports, 14, 1389-1396. https://doi.org/10.3892/mmr.2016.5359
MLA
Shang, J., Wang, H., Fan, X., Shangguan, L., Liu, H."A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells". Molecular Medicine Reports 14.2 (2016): 1389-1396.
Chicago
Shang, J., Wang, H., Fan, X., Shangguan, L., Liu, H."A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells". Molecular Medicine Reports 14, no. 2 (2016): 1389-1396. https://doi.org/10.3892/mmr.2016.5359
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team