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Article Open Access

Isolation, culture, purification and ultrastructural investigation of cardiac telocytes

  • Authors:
    • Yan‑Yan Li
    • Song Zhang
    • Yi‑Gang Li
    • Yan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Diseases, Xinhua Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200092, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1194-1200
    |
    Published online on: June 10, 2016
       https://doi.org/10.3892/mmr.2016.5386
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Abstract

Telocytes (TCs), a novel type of stromal cells, are crucial to cardiac renovation and regeneration. To dissect the pathophysiological effects of cardiac TCs in heart disease, it is essential to develop an effective method to isolate, culture, purify and characterize these cells. In the present study, cardiac TCs were isolated from the hearts of rats by enzymatic digestion. Histology and CD34/PDGFRα expression by flow cytometric assay were used to characterize the cultured cardiac TCs, which were purified by flow cytometric sorting and confirmed by immunofluorescence and electron microscopy. Typical TCs were observed in primary culture, with these exhibiting typical fusiform cell bodies with long moniliform telopodes. Based on flow cytometric sorting with antibodies to CD34 and PDGFRα, there was a substantial increase in the purity of cardiac TCs. Furthermore, immunofluorescence demonstrated that almost all the sorted TCs expressed vimentin, a marker of TCs. Moreover, electron micrographs showed typical TCs based on their ultrastructural features. Using this method, we developed a reproducible protocol for the isolation and purification of cardiac TCs from rat hearts, which yielded TCs with typical characteristics.
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1 

Popescu LM and Faussone-Pellegrini MS: TELOCYTES - a case of serendipity: The winding way from Interstitial Cells of Cajal (ICC), via Interstitial Cajal-Like Cells (ICLC) to TELOCYTES. J Cell Mol Med. 14:729–740. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Suciu L, Popescu LM, Gherghiceanu M, Regalia T, Nicolescu MI, Hinescu ME and Faussone-Pellegrini MS: Telocytes in human term placenta: Morphology and phenotype. Cells Tissues Organs. 192:325–339. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Kostin S: Myocardial telocytes: A specific new cellular entity. J Cell Mol Med. 14:1917–1921. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Popescu LM, Manole CG, Gherghiceanu M, Ardelean A, Nicolescu MI, Hinescu ME and Kostin S: Telocytes in human epicardium. J Cell Mol Med. 14:2085–2093. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Gherghiceanu M, Manole CG and Popescu LM: Telocytes in endocardium: Electron microscope evidence. J Cell Mol Med. 14:2330–2334. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Rusu MC, Jianu AM, Mirancea N, Didilescu AC, Mănoiu VS and Păduraru D: Tracheal telocytes. J Cell Mol Med. 16:401–405. 2012. View Article : Google Scholar

7 

Zheng Y, Zhu T, Lin M, Wu D and Wang X: Telocytes in the urinary system. J Tranl Med. 10:1882012. View Article : Google Scholar

8 

Faussone-Pellegrini MS and Bani D: Relationships between telocytes and cardiomyocytes during pre- and post-natal life. J Cell Mol Med. 14:1061–1063. 2010.PubMed/NCBI

9 

Mandache E, Gherghiceanu M, Macarie C, Kostin S and Popescu LM: Telocytes in human isolated atrial amyloidosis: Ultrastructural remodelling. J Cell Mol Med. 14:2739–2747. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Yang Y, Sun W, Wu SM, Xiao J and Kong X: Telocytes in human heart valves. J Cell Mol Med. 18:759–765. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Gherghiceanu M and Popescu LM: Cardiac telocytes-their junctions and functional implications. Cell Tissue Res. 348:265–279. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Barile L, Lionetti V, Cervio E, Matteucci M, Gherghiceanu M, Popescu LM, Torre T, Siclari F, Moccetti T and Vassalli G: Extracellular vesicles from human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction. Cardiovasc Res. 103:530–541. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Gherghiceanu M and Popescu LM: Cardiomyocyte precursors and telocytes in epicardial stem cell niche: Electron microscope images. J Cell Mol Med. 14:871–877. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Zhao B, Chen S, Liu J, Yuan Z, Qi X, Qin J, Zheng X, Shen X, Yu Y, Qnin TJ, et al: Cardiac telocytes were decreased during myocardial infarction and their therapeutic effects for ischaemic heart in rat. J Cell Mol Med. 17:123–133. 2013. View Article : Google Scholar

15 

Zhao B, Liao Z, Chen S, Yuan Z, Yilin C, Lee KK, Qi X, Shen X, Zheng X, Quinn T and Cai D: Intramyocardial transplantation of cardiac telocytes decreases myocardial infarction and improves post-infarcted cardiac function in rats. J Cell Mol Med. 18:780–789. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Cretoiu SM and Popescu LM: Telocytes revisited. Biomo Concepts. 5:353–369. 2014. View Article : Google Scholar

17 

Bei Y, Zhou Q, Fu S, Lv D, Chen P, Chen Y, Wang F and Xiao J: Cardiac telocytes and fibroblasts in primary culture: Different morphologies and immunophenotypes. PLoS One. 10:e01159912015. View Article : Google Scholar : PubMed/NCBI

18 

Vannucchi MG, Traini C, Manetti M, Ibba-Manneschi L and Faussone-Pellegrini MS: Telocytes express PDGFRα in the human gastrointestinal tract. J Cell Mol Med. 17:1099–1108. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Milia AF, Ruffo M, Manetti M, Rosa I, Conte D, Fazi M, Messerini L and Ibba-Manneschi L: Telocytes in Crohn's disease. J Cell Mol Med. 17:1525–1536. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Mou Y, Wang Y, Li J, Lü S, Duan C, Du Z, Yang G, Chen W, Zhao S, Zhou J and Wang C: Immunohistochemical characterization and functional identification of mammary gland telocytes in the self-assembly of reconstituted breast cancer tissue in vitro. J Cell Mol Med. 17:65–75. 2013. View Article : Google Scholar

21 

Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R and Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 15:c305–c313. 2006. View Article : Google Scholar

22 

Kim JH, Choi SC, Park CY, Park JH, Choi JH, Joo HJ, Hong SJ and Lim DS: Transplantation of immortalized CD34+ and CD34− adipose-derived stem cells improve cardiac function and mitigate systemic pro-inflammatory responses. PLoS One. 11:e01478532016. View Article : Google Scholar

23 

Vannucchi MG, Traini C, Guasti D, Del Popolo G and Faussone-Pellegrini MS: Telocytes subtypes in human urinary bladder. J Cell Mol Med. 18:2000–2008. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Cismaşiu VB and Popescu LM: Telocytes transfer extracellular vesicles loaded with microRNAs to stem cells. J Cell Mol Med. 19:351–358. 2015. View Article : Google Scholar

25 

Andrae J, Gallini R and Betsholtz C: Role of platelet-derived growth factors in physiology and medicine. Genes Dev. 22:1276–1312. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Tallquist MD and Soriano P: Cell autonomous requirement for PDGFRalpha in populations of cranial and cardiac neural crest cells. Development. 130:507–518. 2003. View Article : Google Scholar

27 

Heinrich MC, Corless CL, Duensing A, McGreevey L, Chen CJ, Joseph N, Singer S, Griffith DJ, Haley A, Town A, et al: PDGFRA activating mutations in gastrointestinal stromal tumors. Science. 299:708–710. 2003. View Article : Google Scholar : PubMed/NCBI

28 

Zhou Q, Wei L, Zhong C, Fu S, Bei Y, Huică RI, Wang F and Xiao J: Cardiac telocytes are double positive for CD34/PDGFR-α. J Cell Mol Med. 19:2036–2042. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Chen Y, Li P, Huang PH, Xie Y, Mai JD, Wang L, Nguyen NT and Huang TJ: Rare cell isolation and analysis in microfluidics. Lab Chip. 14:626–645. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Spangrude GJ, Heimfeld S and Weissman IL: Purification and characterization of mouse hematopoietic stem cells. Science. 241:58–62. 1988. View Article : Google Scholar : PubMed/NCBI

31 

Tomlinson MJ, Tomlinson S, Yang XB and Kirkham J: Cell separation: Terminology and practical considerations. J Tissue Eng. 4:20417314124726902013. View Article : Google Scholar : PubMed/NCBI

32 

Schmitz B, Radbruch A, Kümmel T, Wickenhauser C, Korb H, Hansmann ML, Thiele J and Fischer R: Magnetic activated cell sorting (MACS)-a new immunomagnetic method for megakaryocytic cell isolation: Comparison of different separation techniques. Eur J Haematol. 52:267–275. 1994. View Article : Google Scholar : PubMed/NCBI

33 

Grützkau A and Radbruch A: Small but mighty: How the MACS-technology based on nanosized superparamagnetic particles has helped to analyze the immune system within the last 20 years. Cytometry A. 77:643–647. 2010. View Article : Google Scholar

34 

Wilkerson MJ: Principles and applications of flow cytometry and cell sorting in companion animal medicine. Vet Clin North Am Small Anim Pract. 42:53–71. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Suciu L, Nicolescu MI and Popescu LM: Cardiac telocytes: Serial dynamic images in cell culture. J Cell Mol Med. 14:2687–2692. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Zhou J, Wang Y, Zhu P, Sun H, Mou Y, Duan C, Yao A, Lv S and Wang C: Distribution and characteristics of telocytes as nurse cells in the architectural organization of engineered heart tissues. Sci China Life Sci. 57:241–247. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Galiger C, Kostin S, Golec A, Ahlbrecht K, Becker S, Gherghiceanu M, Popescu LM, Morty RE, Seeger W and Voswinckel R: Phenotypical and ultrastructural features of Oct4-positive cells in the adult mouse lung. J Cell Mol Med. 18:1321–1333. 2014. View Article : Google Scholar : PubMed/NCBI

38 

Kostin S and Popescu LM: A distinct type of cell in myocardium: Interstitial Cajal-like cells (ICLCs). J Cell Mol Med. 13:295–308. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Cretoiu D, Hummel E, Zimmermann H, Gherghiceanu M and Popescu LM: Human cardiac telocytes: 3D imaging by FIB-SEM tomography. J Cell Mol Med. 18:2157–2164. 2014. View Article : Google Scholar : PubMed/NCBI

40 

Fertig ET, Gherghiceanu M and Popescu LM: Extracellular vesicles release by cardiac telocytes: Electron microscopy and electron tomography. J Cell Mol Med. 18:1938–1943. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li YY, Zhang S, Li YG and Wang Y: Isolation, culture, purification and ultrastructural investigation of cardiac telocytes. Mol Med Rep 14: 1194-1200, 2016.
APA
Li, Y., Zhang, S., Li, Y., & Wang, Y. (2016). Isolation, culture, purification and ultrastructural investigation of cardiac telocytes. Molecular Medicine Reports, 14, 1194-1200. https://doi.org/10.3892/mmr.2016.5386
MLA
Li, Y., Zhang, S., Li, Y., Wang, Y."Isolation, culture, purification and ultrastructural investigation of cardiac telocytes". Molecular Medicine Reports 14.2 (2016): 1194-1200.
Chicago
Li, Y., Zhang, S., Li, Y., Wang, Y."Isolation, culture, purification and ultrastructural investigation of cardiac telocytes". Molecular Medicine Reports 14, no. 2 (2016): 1194-1200. https://doi.org/10.3892/mmr.2016.5386
Copy and paste a formatted citation
x
Spandidos Publications style
Li YY, Zhang S, Li YG and Wang Y: Isolation, culture, purification and ultrastructural investigation of cardiac telocytes. Mol Med Rep 14: 1194-1200, 2016.
APA
Li, Y., Zhang, S., Li, Y., & Wang, Y. (2016). Isolation, culture, purification and ultrastructural investigation of cardiac telocytes. Molecular Medicine Reports, 14, 1194-1200. https://doi.org/10.3892/mmr.2016.5386
MLA
Li, Y., Zhang, S., Li, Y., Wang, Y."Isolation, culture, purification and ultrastructural investigation of cardiac telocytes". Molecular Medicine Reports 14.2 (2016): 1194-1200.
Chicago
Li, Y., Zhang, S., Li, Y., Wang, Y."Isolation, culture, purification and ultrastructural investigation of cardiac telocytes". Molecular Medicine Reports 14, no. 2 (2016): 1194-1200. https://doi.org/10.3892/mmr.2016.5386
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