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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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August-2016 Volume 14 Issue 2

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International Journal of Molecular Medicine

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Article

Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion

  • Authors:
    • Jian‑Bo Yu
    • Hao Jiang
    • Ren‑Ya Zhan
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
  • Pages: 1263-1268
    |
    Published online on: June 10, 2016
       https://doi.org/10.3892/mmr.2016.5391
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Abstract

Upregulation of the Notch signaling pathway in cancer stem cells and side population (SP) cells has a major role in maintenance, self-renewal and chemoresistance. The present study isolated a cancer stem cell-like SP accounting for 4.1% of a glioblastoma cell population using a Hoechst 33342 dye exclusion assay. In this glioblastoma SP, the expression of of Notch1 signaling proteins Notch1 intracellular domain and Hes‑1 was markedly upregulated. Furthermore, knockdown of Notch1 by RNA interference significantly diminished the neurosphere formation ability, self‑renewal and chemoresistance of the SP cells. In addition, the expression of the stem‑cell surface genes Oct‑4, Sox2 and Nanog in SP cells was significantly reduced and the sensitivity to the SP cells to chemotherapeutics was enhanced following Notch1 knockdown. In conclusion, the results of the present study suggested that upregulation of Notch1 is involved in the chemotherapy resistance and tumor recurrence of glioblastoma. Hence, the development of novel anti‑cancer drugs targeting the Notch1 signaling pathway may be a promising strategy for curing glioblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Yu JB, Jiang H and Zhan RY: Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion. Mol Med Rep 14: 1263-1268, 2016.
APA
Yu, J., Jiang, H., & Zhan, R. (2016). Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion. Molecular Medicine Reports, 14, 1263-1268. https://doi.org/10.3892/mmr.2016.5391
MLA
Yu, J., Jiang, H., Zhan, R."Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion". Molecular Medicine Reports 14.2 (2016): 1263-1268.
Chicago
Yu, J., Jiang, H., Zhan, R."Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion". Molecular Medicine Reports 14, no. 2 (2016): 1263-1268. https://doi.org/10.3892/mmr.2016.5391
Copy and paste a formatted citation
x
Spandidos Publications style
Yu JB, Jiang H and Zhan RY: Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion. Mol Med Rep 14: 1263-1268, 2016.
APA
Yu, J., Jiang, H., & Zhan, R. (2016). Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion. Molecular Medicine Reports, 14, 1263-1268. https://doi.org/10.3892/mmr.2016.5391
MLA
Yu, J., Jiang, H., Zhan, R."Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion". Molecular Medicine Reports 14.2 (2016): 1263-1268.
Chicago
Yu, J., Jiang, H., Zhan, R."Aberrant Notch signaling in glioblastoma stem cells contributes to tumor recurrence and invasion". Molecular Medicine Reports 14, no. 2 (2016): 1263-1268. https://doi.org/10.3892/mmr.2016.5391
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