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August-2016 Volume 14 Issue 2

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CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma

  • Authors:
    • Wei Dai
    • Huilai Miao
    • Shuo Fang
    • Tao Fang
    • Nianping Chen
    • Mingyi Li
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China, Department of Endocrinology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, P.R. China
    Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1509-1514
    |
    Published online on: June 17, 2016
       https://doi.org/10.3892/mmr.2016.5410
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Abstract

Aberrant expression of CDKN3 may be involved in carcinogenesis of liver cancer. The effect of CDKN3 on tumorigenesis and the molecular mechanisms involved have not been fully elucidated. Immunohistochemistry was performed to detect CDKN3 expression levels in tumor tissues. CDKN3 siRNA was used to knockdown CDKN3 in QGY7701 hepatocellular carcinoma (HCC) cells. Colony formation assay was used to measure the clonogenic capacity of the tumor cells. Cell viability was determined by MTT assay. Logistic regression was performed to analyze the association between CDKN3 expression level and the HCC clinical pathology index. The CDKN3 expression level was significantly decreased in HCC tumor tissues compared with normal liver tissue and liver cirrhosis tissue. Additionally, CDKN3 expression was negatively‑associated with the pathological stage of the tumor. Inhibition of CKDN3 promoted the clonogenic capacity and chemotherapeutic tolerance in HCC tissues compared with controls. Knockdown of CDKN3 resulted in downregulation of p53 and p21 protein levels, whereas, AKT serine/threonine kinase 1 expression was upregulated. Thus, CDKN3 expression may reduce the survival of tumor cells and alter the sensitivity to therapeutic agents via the AKT/P53/P21 signaling pathway. Therefore, CDKN3 may be involved in tumor differentiation and self-renewal.
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1 

Shiraha H, Yamamoto K and Namba M: Human hepatocyte carcinogenesis (review). Int J Oncol. 42:1133–1138. 2013.PubMed/NCBI

2 

Zimonjic DB and Popescu NC: Role of DLC1 tumor suppressor gene and MYC oncogene in pathogenesis of human hepatocellular carcinoma: Potential prospects for combined targeted therapeutics (review). Int J Oncol. 41:393–406. 2012.PubMed/NCBI

3 

Kirstein MM and Vogel A: The pathogenesis of hepatocellular carcinoma. Dig Dis. 32:545–553. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Meng X, Franklin DA, Dong J and Zhang Y: MDM2-p53 pathway in hepatocellular carcinoma. Cancer Res. 74:7161–7167. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Kawaguchi M and Kataoka H: Mechanisms of hepatocyte growth factor activation in cancer tissues. Cancers (Basel). 6:1890–1904. 2014. View Article : Google Scholar

6 

Xie B and Dong JH: HGF/c-Met and metastasis of hepatocellular carcinoma. Zhonghua Gan Zang Bing Za Zhi. 13:396–398. 2005.In Chinese. PubMed/NCBI

7 

Espinosa AM, Alfaro A, Roman-Basaure E, Guardado-Estrada M, Palma Í, Serralde C, Medina I, Juárez E, Bermúdez M, Márquez E, et al: Mitosis is a source of potential markers for screening and survival and therapeutic targets in cervical cancer. PLoS One. 8:e559752013. View Article : Google Scholar : PubMed/NCBI

8 

Lai MW, Chen TC, Pang ST and Yeh CT: Overexpression of cyclin-dependent kinase-associated protein phosphatase enhances cell proliferation in renal cancer cells. Urol Oncol. 30:871–878. 2012. View Article : Google Scholar

9 

Li T, Xue H, Guo Y and Guo K: CDKN3 is an independent prognostic factor and promotes ovarian carcinoma cell proliferation in ovarian cancer. Oncol Rep. 31:1825–1831. 2014.PubMed/NCBI

10 

Taylor KJ, Sims AH, Liang L, Faratian D, Muir M, Walker G, Kuske B, Dixon JM, Cameron DA, Harrison DJ and Langdon SP: Dynamic changes in gene expression in vivo predict prognosis of tamoxifen-treated patients with breast cancer. Breast Cancer Res. 12:R392010. View Article : Google Scholar : PubMed/NCBI

11 

Drozdov I, Bornschein J, Wex T, Valeyev NV, Tsoka S and Malfertheiner P: Functional and topological properties in hepa-tocellular carcinoma transcriptome. PLoS One. 7:e355102012. View Article : Google Scholar

12 

Xing C, Xie H, Zhou L, Zhou W, Zhang W, Ding S, Wei B, Yu X, Su R and Zheng S: Cyclin-dependent kinase inhibitor 3 is over-expressed in hepatocellular carcinoma and promotes tumor cell proliferation. Biochem Biophys Res Commun. 420:29–35. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Li H, Jiang X, Yu Y, Huang W, Xing H, Agar NY, Yang HW, Yang B, Carroll RS and Johnson MD: KAP regulates ROCK2 and Cdk2 in an RNA-activated glioblastoma invasion pathway. Oncogene. 34:1432–1441. 2015. View Article : Google Scholar

14 

Yeh CT, Lu SC, Chen TC, Peng CY and Liaw YF: Aberrant transcripts of the cyclin-dependent kinase-associated protein phosphatase in hepatocellular carcinoma. Cancer Res. 60:4697–4700. 2000.PubMed/NCBI

15 

Nalepa G, Barnholtz-Sloan J, Enzor R, Dey D, He Y, Gehlhausen JR, Lehmann AS, Park SJ, Yang Y, Yang X, et al: The tumor suppressor CDKN3 controls mitosis. J Cell Biol. 201:997–1012. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Hou J, Liu Z and Gu F: Epidemiology and prevention of hepatitis B virus infection. Int J Med Sci. 2:50–57. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Yu Y, Jiang X, Schoch BS, Carroll RS, Black PM and Johnson MD: Aberrant splicing of cyclin-dependent kinase-associated protein phosphatase KAP increases proliferation and migration in glioblastoma. Cancer Res. 67:130–138. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Lee SW, Reimer CL, Fang L, Iruela-Arispe ML and Aaronson SA: Overexpression of kinase-associated phosphatase (KAP) in breast and prostate cancer and inhibition of the transformed phenotype by antisense KAP expression. Mol Cell Biol. 20:1723–1732. 2000. View Article : Google Scholar : PubMed/NCBI

19 

McCubrey JA, Steelman LS, Franklin RA, Abrams SL, Chappell WH, Wong EW, Lehmann BD, Terrian DM, Basecke J, Stivala F, et al: Targeting the RAF/MEK/ERK, PI3 K/AKT and p53 pathways in hematopoietic drug resistance. Adv Enzyme Regul. 47:64–103. 2007. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Dai W, Miao H, Fang S, Fang T, Chen N and Li M: CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma. Mol Med Rep 14: 1509-1514, 2016.
APA
Dai, W., Miao, H., Fang, S., Fang, T., Chen, N., & Li, M. (2016). CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma. Molecular Medicine Reports, 14, 1509-1514. https://doi.org/10.3892/mmr.2016.5410
MLA
Dai, W., Miao, H., Fang, S., Fang, T., Chen, N., Li, M."CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma". Molecular Medicine Reports 14.2 (2016): 1509-1514.
Chicago
Dai, W., Miao, H., Fang, S., Fang, T., Chen, N., Li, M."CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma". Molecular Medicine Reports 14, no. 2 (2016): 1509-1514. https://doi.org/10.3892/mmr.2016.5410
Copy and paste a formatted citation
x
Spandidos Publications style
Dai W, Miao H, Fang S, Fang T, Chen N and Li M: CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma. Mol Med Rep 14: 1509-1514, 2016.
APA
Dai, W., Miao, H., Fang, S., Fang, T., Chen, N., & Li, M. (2016). CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma. Molecular Medicine Reports, 14, 1509-1514. https://doi.org/10.3892/mmr.2016.5410
MLA
Dai, W., Miao, H., Fang, S., Fang, T., Chen, N., Li, M."CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma". Molecular Medicine Reports 14.2 (2016): 1509-1514.
Chicago
Dai, W., Miao, H., Fang, S., Fang, T., Chen, N., Li, M."CDKN3 expression is negatively associated with pathological tumor stage and CDKN3 inhibition promotes cell survival in hepatocellular carcinoma". Molecular Medicine Reports 14, no. 2 (2016): 1509-1514. https://doi.org/10.3892/mmr.2016.5410
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