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Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis

  • Authors:
    • Magdy El‑Salhy
    • Kazuo Umezawa
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology, Department of Medicine, Stord Helse‑Fonna Hospital, 5416 Stord, Norway, Department of Molecular Target Medicine, School of Aichi Medical University, School of Medicine, Nagakute, Aichi 480‑1195, Japan
    Copyright: © El‑Salhy et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1515-1522
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    Published online on: June 27, 2016
       https://doi.org/10.3892/mmr.2016.5444
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Abstract

Interactions between intestinal neuroendocrine peptides/amines and the immune system appear to have an important role in the pathophysiology of inflammatory bowel disease (IBD). The present study investigated the effects of activator protein (AP)‑1 and nuclear factor (NF)‑κB inhibitors on inflammation‑induced alterations in enteroendocrine cells. A total of 48 male Wistar rats were divided into the following four groups (n=12 rats/group): Control, trinitrobenzene sulfonic acid (TNBS)‑induced colitis only (TNBS group), TNBS‑induced colitis with 3‑[(dodecylthiocarbonyl)-methyl]-glutarimide (DTCM‑G) treatment (DTCM‑G group), and TNBS‑induced colitis with dehydroxymethylepoxyquinomicin (DHMEQ) treatment (DHMEQ group). A total of 3 days following administration of TNBS, the rats were treated as follows: The control and TNBS groups received 0.5 ml vehicle (0.5% carboxymethyl cellulose; CMC), respectively; the DTCM‑G group received DTCM‑G (20 mg/kg body weight) in 0.5% CMC; and the DHMEQ group received DHMEQ (15 mg/kg body weight) in 0.5% CMC. All injections were performed intraperitoneally twice daily for 5 days. The rats were sacrificed, and tissue samples obtained from the colon were examined histopathologically and immunohistochemically. Inflammation was evaluated using a scoring system. In addition, the sections were immunostained for chromogranin A (CgA), serotonin, peptide YY (PYY), oxyntomodulin, pancreatic polypeptide (PP) and somatostatin, and immunostaining was quantified using image‑analysis software. The density of cells expressing CgA, PYY and PP was significantly lower in the TNBS group compared with in the control group, whereas the density of cells expressing serotonin, oxyntomodulin and somatostatin was significantly higher in the TNBS group compared with in the control group. None of the endocrine cell types differed significantly between the control group and either the DTCM‑G or DHMEQ groups. All of the colonic endocrine cell types were affected in rats with TNBS‑induced colitis. The expression density of these endocrine cell types was restored to control levels following treatment with AP‑1 or NF‑κB inhibitors. These results indicated that the immune system and enteroendocrine cells interact in IBD.
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Copy and paste a formatted citation
Spandidos Publications style
El‑Salhy M and Umezawa K: Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis. Mol Med Rep 14: 1515-1522, 2016.
APA
El‑Salhy, M., & Umezawa, K. (2016). Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis. Molecular Medicine Reports, 14, 1515-1522. https://doi.org/10.3892/mmr.2016.5444
MLA
El‑Salhy, M., Umezawa, K."Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis". Molecular Medicine Reports 14.2 (2016): 1515-1522.
Chicago
El‑Salhy, M., Umezawa, K."Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis". Molecular Medicine Reports 14, no. 2 (2016): 1515-1522. https://doi.org/10.3892/mmr.2016.5444
Copy and paste a formatted citation
x
Spandidos Publications style
El‑Salhy M and Umezawa K: Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis. Mol Med Rep 14: 1515-1522, 2016.
APA
El‑Salhy, M., & Umezawa, K. (2016). Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis. Molecular Medicine Reports, 14, 1515-1522. https://doi.org/10.3892/mmr.2016.5444
MLA
El‑Salhy, M., Umezawa, K."Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis". Molecular Medicine Reports 14.2 (2016): 1515-1522.
Chicago
El‑Salhy, M., Umezawa, K."Effects of AP‑1 and NF‑κB inhibitors on colonic endocrine cells in rats with TNBS‑induced colitis". Molecular Medicine Reports 14, no. 2 (2016): 1515-1522. https://doi.org/10.3892/mmr.2016.5444
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