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Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16

  • Authors:
    • Weiwei Fan
    • Shuang Chang
    • Xiumei Shan
    • Dejun Gao
    • Steven Qian Zhang
    • Jin Zhang
    • Nan Jiang
    • Duan Ma
    • Zuohua Mao
  • View Affiliations / Copyright

    Affiliations: Department of Parasitology and Microbiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China, Key Laboratory of Molecular Medicine, Ministry of Education, Department of Biochemistry and Molecular Biology, Institutes of Biomedical Sciences, School of Basic Medical Sciences, Fudan University, Shanghai 200032, P.R. China
    Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4099-4108
    |
    Published online on: September 21, 2016
       https://doi.org/10.3892/mmr.2016.5758
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Abstract

Toxoplasma rhoptry protein 16 (ROP16) is crucial in the host-pathogen interaction by acting as a virulent factor during invasion. To improve understanding of the molecular function underlying the effect of ROP16 on host cells, the present study analyzed the transcriptional profile of genes in the ROP16‑transfected SH‑SY5Y human neuroblastoma cell line. The transcriptional profile of the SH‑SY5Y human neuroblastoma cell line overexpressing ROP16 were determined by microarray analysis in order to determine the host neural cell response to the virulent factor. Functional analysis was performed using the Protein Analysis Through Evolutionary Relationships classification system. The ToppGene Suite was used to select candidate genes from the differentially expressed genes. A protein‑protein interaction network was constructed using Cytoscape software according to the interaction associations determined using the Search Tool for the Retrieval of Interacting Genes/Proteins. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis of the selected genes confirmed the results of the microarray. The results showed that 383 genes were differentially expressed in response to ROP16 transfection, of which 138 genes were upregulated and 245 genes were downregulated. Functional analysis indicated that the differentially expressed genes (DEGs) were involved in several biological processes, including developmental process, biological regulation and apoptotic process. A total of 15 candidate genes from the DEGs were screened using the ToppGene Suite. No significant differences in expression were observed between the RT‑qPCR data and the microarray data. Transfection with ROP16 resulted in alterations of several biological processes, including nervous system development, apoptosis and transcriptional regulation. Several genes, including CXCL12, BAI1, ZIC2, RBMX, RASSF6, MAGE‑A6 and HOX, were identified as significant DEGs. Taken together, these results may contribute to understanding the mechanisms underlying the functions of ROP16 and provide scope for further investigation of the pathogenesis of Toxoplasma gondii.
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1 

John B, Ricart B, Wojno ED Tait, Harris TH, Randall LM, Christian DA, Gregg B, De Almeida DM, Weninger W, Hammer DA and Hunter CA: Analysis of behavior and trafficking of dendritic cells within the brain during toxoplasmic encephalitis. PLoS Pathog. 7:e10022462011. View Article : Google Scholar : PubMed/NCBI

2 

Montoya JG and Remington JS: Management of Toxoplasma gondii infection during pregnancy. Clin Infect Dis. 47:554–566. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Tanaka S, Nishimura M, Ihara F, Yamagishi J, Suzuki Y and Nishikawa Y: Transcriptome analysis of mouse brain infected with Toxoplasma gondii. Infect Immun. 81:3609–3619. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Boothroyd JC: Have it your way: How polymorphic, injected kinases and pseudokinases enable Toxoplasma to subvert host defenses. PLoS Pathog. 9:e10032962013. View Article : Google Scholar : PubMed/NCBI

5 

Dubremetz JF: Rhoptries are major players in Toxoplasma gondii invasion and host cell interaction. Cellular Microbiol. 9:841–848. 2007. View Article : Google Scholar

6 

Mueller C, Klages N, Jacot D, Santos JM, Cabrera A, Gilberger TW, Dubremetz JF and Soldati-Favre D: The Toxoplasma protein ARO mediates the apical positioning of rhoptry organelles, a prerequisite for host cell invasion. Cell Host Microbe. 13:289–301. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Taylor S, Barragan A, Su C, Fux B, Fentress SJ, Tang K, Beatty WL, Hajj HE, Jerome M, Behnke MS, et al: A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii. Science. 314:1776–1780. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Boothroyd JC and Dubremetz JF: Kiss and spit: The dual roles of Toxoplasma rhoptries. Nat Rev Microbiol. 6:79–88. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Saeij JP, Coller S, Boyle JP, Jerome ME, White MW and Boothroyd JC: Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue. Nature. 445:324–327. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Jensen KD, Hu K, Whitmarsh RJ, Hassan MA, Julien L, Lu D, Chen L, Hunter CA and Saeij JP: Toxoplasma gondii rhoptry 16 kinase promotes host resistance to oral infection and intestinal inflammation only in the context of the dense granule protein GRA15. Infect Immun. 81:2156–2167. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Mi H, Muruganujan A and Thomas PD: PANTHER in 2013: Modeling the evolution of gene function, and other gene attributes, in the context of phylogenetic trees. Nucleic Acids Res. 41:(Database Issue). D377–D386. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Mi H, Muruganujan A, Casagrande JT and Thomas PD: Large-scale gene function analysis with the PANTHER classification system. Nature Protoc. 8:1551–1566. 2013. View Article : Google Scholar

13 

Chen J, Bardes EE, Aronow BJ and Jegga AG: ToppGene suite for gene list enrichment analysis and candidate gene prioritization. Nucleic Acids Res (Web Server Issue). 37:W305–W311. 2009. View Article : Google Scholar

14 

Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, Huerta-Cepas J, Simonovic M, Roth A, Santos A, Tsafou KP, et al: STRING v10: Protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res. 43:(Database Issue). D447–D452. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B and Ideker T: Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res. 13:2498–2504. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Bader GD and Hogue CW: An automated method for finding molecular complexes in large protein interaction networks. BMC Bioinformatics. 4:22003. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Chen J, Xu H, Aronow BJ and Jegga AG: Improved human disease candidate gene prioritization using mouse phenotype. BMC Bioinformatics. 8:3922007. View Article : Google Scholar : PubMed/NCBI

19 

Dugas JC, Mandemakers W, Rogers M, Ibrahim A, Daneman R and Barres BA: A novel purification method for CNS projection neurons leads to the identification of brain vascular cells as a source of trophic support for corticospinal motor neurons. J Neurosci. 28:8294–8305. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Oh JW, Olman M and Benveniste EN: CXCL12-mediated induction of plasminogen activator inhibitor-1 expression in human CXCR4 positive astroglioma cells. Biol Pharm Bull. 32:573–577. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Carruthers VB and Suzuki Y: Effects of Toxoplasma gondii infection on the brain. Schizophr Bull. 33:745–751. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Mori K, Kanemura Y, Fujikawa H, Nakano A, Ikemoto H, Ozaki I, Matsumoto T, Tamura K, Yokota M and Arita N: Brain-specific angiogenesis inhibitor 1 (BAI1) is expressed in human cerebral neuronal cells. Neurosci Res. 43:69–74. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Zhu D, Li C, Swanson AM, Villalba RM, Guo J, Zhang Z, Matheny S, Murakami T, Stephenson JR, Daniel S, et al: BAI1 regulates spatial learning and synaptic plasticity in the hippocampus. J Clin Invest. 125:14975082015. View Article : Google Scholar

24 

Nagai T, Aruga J, Minowa O, Sugimoto T, Ohno Y, Noda T and Mikoshiba K: Zic2 regulates the kinetics of neurulation. Proc Natl Acad Sci USA. 97:1618–1623. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Tsend-Ayush E, O'Sullivan LA, Grützner FS, Onnebo SM, Lewis RS, Delbridge ML, Graves JA Marshall and Ward AC: RBMX gene is essential for brain development in zebrafish. Dev Dyn. 234:682–688. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Wang T, Zhou J, Gan X, Wang H, Ding X, Chen L, Wang Y, DU J, Shen J and Yu L: Toxoplasma gondii induce apoptosis of neural stem cells via endoplasmic reticulum stress pathway. Parasitology. 141:988–995. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Nyoman AD and Lüder CG: Apoptosis-like cell death pathways in the unicellular parasite Toxoplasma gondii following treatment with apoptosis inducers and chemotherapeutic agents: A proof-of-concept study. Apoptosis. 18:664–680. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Iwasa H, Kudo T, Maimaiti S, Ikeda M, Maruyama J, Nakagawa K and Hata Y: The RASSF6 tumor suppressor protein regulates apoptosis and the cell cycle via MDM2 protein and p53 protein. J Biol Chem. 288:30320–30329. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Marcar L, MacLaine NJ, Hupp TR and Meek DW: Mage-A cancer/testis antigens inhibit p53 function by blocking its interaction with chromatin. Cancer Res. 70:10362–10370. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Cantile M, Procino A, D'armiento M, Cindolo L and Cillo C: HOX gene network is involved in the transcriptional regulation of in vivo human adipogenesis. J Cell Physiol. 194:225–236. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Fan W, Chang S, Shan X, Gao D, Zhang SQ, Zhang J, Jiang N, Ma D and Mao Z: Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16. Mol Med Rep 14: 4099-4108, 2016.
APA
Fan, W., Chang, S., Shan, X., Gao, D., Zhang, S.Q., Zhang, J. ... Mao, Z. (2016). Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16. Molecular Medicine Reports, 14, 4099-4108. https://doi.org/10.3892/mmr.2016.5758
MLA
Fan, W., Chang, S., Shan, X., Gao, D., Zhang, S. Q., Zhang, J., Jiang, N., Ma, D., Mao, Z."Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16". Molecular Medicine Reports 14.5 (2016): 4099-4108.
Chicago
Fan, W., Chang, S., Shan, X., Gao, D., Zhang, S. Q., Zhang, J., Jiang, N., Ma, D., Mao, Z."Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16". Molecular Medicine Reports 14, no. 5 (2016): 4099-4108. https://doi.org/10.3892/mmr.2016.5758
Copy and paste a formatted citation
x
Spandidos Publications style
Fan W, Chang S, Shan X, Gao D, Zhang SQ, Zhang J, Jiang N, Ma D and Mao Z: Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16. Mol Med Rep 14: 4099-4108, 2016.
APA
Fan, W., Chang, S., Shan, X., Gao, D., Zhang, S.Q., Zhang, J. ... Mao, Z. (2016). Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16. Molecular Medicine Reports, 14, 4099-4108. https://doi.org/10.3892/mmr.2016.5758
MLA
Fan, W., Chang, S., Shan, X., Gao, D., Zhang, S. Q., Zhang, J., Jiang, N., Ma, D., Mao, Z."Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16". Molecular Medicine Reports 14.5 (2016): 4099-4108.
Chicago
Fan, W., Chang, S., Shan, X., Gao, D., Zhang, S. Q., Zhang, J., Jiang, N., Ma, D., Mao, Z."Transcriptional profile of SH-SY5Y human neuroblastoma cells transfected by Toxoplasma rhoptry protein 16". Molecular Medicine Reports 14, no. 5 (2016): 4099-4108. https://doi.org/10.3892/mmr.2016.5758
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